Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):173-182 | 10.5507/bp.2016.012
Dysfunction of renal mitochondria can be observed in the pathogenesis of a number of renal disorders. Proteomic methods are used for analysis of mitochondrial proteins and protein complexes in animal kidney models and humans in the course of various pathological states. Proteomics of renal mitochondria are useful for understanding pathology and discovering potential targets for therapy.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):183-194 | 10.5507/bp.2015.050
The global incidence of renal cell cancer is increasing . Knowledge of renal carcinogenesis is one way of improving of treatment options and cancer prevention.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):195-200 | 10.5507/bp.2016.005
In the severe stages of dry eye disease, tear production is insufficient to maintain the health of the front surface of the eye and to enable clear vision. Therapeutic options on suppressing of apoptosis and inflammation are l limited and require further research. This study focuses on the effect of autologous serum treatment by assessing the level of ocular surface apoptosis and treatment outcome.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):201-210 | 10.5507/bp.2015.066
A number of genes associated with the development and function of synapses play significant roles in autism. This review focuses on deficits in synaptic proteins associated with autism and animal genetic models of autism with respect to alterations in selected synaptic proteins such as neuroligins, neurexins and shank proteins.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):211-218 | 10.5507/bp.2016.030
The authors report on the development and preliminary validation of a new prognostic scale (the COPD Prognostic Score - CPS) using a retrospective analysis of the National Emphysema Treatment Trial cohort. The CPS included: the age mMRC dyspnea scale, post-bronchodilator FEV1 (% predicted), hemoglobin level, respiratory emergency admission rate and daily activity. The CPS ranged from 0 to 16, with 16 indicating the poorest prognosis. A one-point increase in the CPS was associated with an increase,in all-cause death (with a HR of 1.28). The preliminary validation suggests that the CPS (new prognostic scale) had a slightly better prognostic ability than the BODE index and the ADO index. Future validation in a prospective cohort is anticipated.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):219-221 | 10.5507/bp.2016.019
This study showed that patients with premature manifestation of coronary artery disease had significantly lower Omentin-1 serum levels than patients with metabolic syndrome and healthy subjects. To the best of their knowledge, the authors report that this is the first study to assess omentin-1 levels in younger patients with premature manifestations of atherosclerosis. Serum omentin-1 appears to be a useful biomarker of coronary artery disease across the whole age spectrum.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):222-230 | 10.5507/bp.2016.013
This study describes a conceptually new system of drug delivery which may increase the plasma circulation time of the hydrolytically labile azanucleoside drugs that are subject to irreversible chemical decomposition in aqueous solution.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):231-237 | 10.5507/bp.2015.054
Pediatric patients with erythrocyte membrane defect and thalassemia trait show disturbed balance between erythropoiesis and iron metabolism. The (hepcidin/ferritin)/sTfR ratio distinguishes patients with erythrocyte membrane defect and the thalassemia trait from healthy populations. These patients should be followed up for possible alterations in erythropoietic activity and potential risk of excessive iron accumulation.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):238-243 | 10.5507/bp.2015.058
This is an investigation on the development of a multilocus calibration material for analysis of somatostatin (SSTR1-5), estrogen (ER1), and dopamine (D2R) receptors in pituitary adenomas. Using this calibrator the authors were able to align all calibration functions, including assays for the transcripts whose expression in the pituitary was very low (SSTR4 and SSTR5). As a result the authors investigated a large group of 206 pituitary tumors with various histo-pathological classification, and defined individual expression profile for postoperative pharmacological treatment with somatostatin analogues and dopamine agonists. The numbers of D2R, ER1, and SSTR1 transcripts were highest in patients with Cushing disease and prolactinomas. SSTR2 expression was significantly higher in acromegaly and thyrotroph adenomas. Significantly more SSTR3 transcripts were found in clinically non-functioning adenomas and acromegaly, and higher levels of SSTR5 were found in patients with acromegaly and prolactinomas.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):244-247 | 10.5507/bp.2016.031
The estimation of serum drug levels enables diagnosis of non-adherence to recommended therapy. The data suggest that non-adherence is a common problem among patients presenting with acute decompensation of chronic heart failure.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):248-256 | 10.5507/bp.2015.067
Pacemaker implantation damages the small area of the myocardium that comes into contact with the electrode tip. This situation leads to slight increases in troponin I levels, resulting in the loss of troponin I's diagnostic role in patients presenting with chest pain shortly after the procedure. Because ECGs can also be influenced during pacing, a diagnostic coronarography is often necessary.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):257-262 | 10.5507/bp.2015.049
The authors describe long-term follow-up of a large, selected and homogenous cohort of patients from a single centre reflecting the course of heart failure with severe left ventricular systolic dysfunction barely modified by comorbidities. Short-term prognosis of heart failure patients could be estimated based on simple parameters available on out-patient examination. Patients presenting with these parameters should be referred to a tertiary centre to be considered for advanced heart failure therapy.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):263-270 | 10.5507/bp.2015.059
In this prospective randomized clinical study PRAGUE-6, the authors collated data from a rarely observed population of high-risk cand idates for direct myocardial revascularisation, an expected early mortality of about 10%. They confirmed that revascuralisation without the use of extracorporal circulation was associated with lower incidence of early major postoperative complications in this subgroup of patients.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):271-275 | 10.5507/bp.2016.001
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):276-279 | 10.5507/bp.2016.029
The results of this study showed the sustained efficiency of ivacaftor on pulmonary function for treatment periods up to six years, with marked reduction in antibiotic use and hospital stays.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):280-286 | 10.5507/bp.2016.011
A unique study within the Czech Republic on the long-term survival of patients with resectable pancreatic cancer in a tertiary centre. It demonstrates the effect of proven histopathological vascular invasion on long-term survival in resectable tumors; a factor that is rarely taken into consideration and compares it to other similar international studies. Postoperative complications were another independent prognostic factor after resections.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):287-297 | 10.5507/bp.2015.052
The article presents original epidemiological data on severe sepsis patients in the Czech Republic. It assesses data collected as part of the EPOSS project - the largest database of patients with severe sepsis in Central Europe. It contains information gathered over a period of almost three years. Based on retrospective data analysis, the paper identifies the combination of diagnostic/therapeutic interventions during the initial 6 hours of severe sepsis most closely related to in-hospital mortality. It identifies interventions that may be, based on statistical assessment, considered as risky or protective in relation to in-hospital mortality. In addition, the paper provides data on about the in-hospital morality of patients with severe sepsis with respect to the care procedure and course. It demonstrates the therapy/diagnosis intervention achievement level according to the Sepsis Surviving Campaign.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):298-304 | 10.5507/bp.2015.062
This study provides a unique view on IE-related complications in Central Europe. The authors found IE episodes caused by S. aureus were associated with increased short-term and long-term mortality. Of all the studied parameters, only systemic embolism and severe sepsis/septic shock predicted in-hospital mortality. No differences in local structural and/or functional complications (cardiac abscess formation, impaired integrity of the valvular mechanism, conduction disturbances or incidence of heart failure) were observed for S. aureus IE and episodes of IE caused by other aetiological agents.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):305-309 | 10.5507/bp.2015.064
This paper confirms the superiority of currently used imaging techniques, low-dose computed tomography (LD-CT) and whole-body magnetic resonance imaging (WB-MRI) over conventional radiography (CR) in assessment of myeloma bone disease (MBD) in a prospectively designed study. The authors conclude that either WB-MRI or LD-CT should be used as preferred imaging techniques in the place of CR in order to detect correctly skeletal involvement in multiple myeloma (MM). Relying solely on CR may underestimate the extent of MBD or even fail to diagnose active MM requiring treatment in a substantial number of patients.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):310-315 | 10.5507/bp.2016.020
This case report and review gives a brief and critical overview of Oromandibular Limb Hypogenesis Syndromes (OLHS) by analyzing the current state of knowledge. A new, simpler classification of OLHS is introduced.