Biomedical papers, 2016 (vol. 160), issue 2

Review articles

Proteomic approaches to the study of renal mitochondria

Zdenek Tuma, Jitka Kuncova, Jan Mares, Martina Grundmanova, Martin Matejovic

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):173-182 | 10.5507/bp.2016.012

Dysfunction of renal mitochondria can be observed in the pathogenesis of a number of renal disorders. Proteomic methods are used for analysis of mitochondrial proteins and protein complexes in animal kidney models and humans in the course of various pathological states. Proteomics of renal mitochondria are useful for understanding pathology and discovering potential targets for therapy.

Renal cell carcinoma: Review of etiology, pathophysiology and risk factors

Nadezda Petejova, Arnost Martinek

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):183-194 | 10.5507/bp.2015.050

The global incidence of renal cell cancer is increasing . Knowledge of renal carcinogenesis is one way of improving of treatment options and cancer prevention.

New-onset diabetes mellitus after renal transplantation

Dominika Goldmannova, David Karasek, Ondrej Krystynik, Josef Zadrazil

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):195-200 | 10.5507/bp.2016.005

In the severe stages of dry eye disease, tear production is insufficient to maintain the health of the front surface of the eye and to enable clear vision. Therapeutic options on suppressing of apoptosis and inflammation are l limited and require further research. This study focuses on the effect of autologous serum treatment by assessing the level of ocular surface apoptosis and treatment outcome.

Synapse alterations in autism: Review of animal model findings

Martina Zatkova, Jan Bakos, Julius Hodosy, Daniela Ostatnikova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):201-210 | 10.5507/bp.2015.066

A number of genes associated with the development and function of synapses play significant roles in autism. This review focuses on deficits in synaptic proteins associated with autism and animal genetic models of autism with respect to alterations in selected synaptic proteins such as neuroligins, neurexins and shank proteins.

Original contributions

Chronic obstructive pulmonary disease prognostic score: A new index

Nobuyuki Horita, Vladimir Koblizek, Marek Plutinsky, Barbora Novotna, Karel Hejduk, Takeshi Kaneko

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):211-218 | 10.5507/bp.2016.030

The authors report on the development and preliminary validation of a new prognostic scale (the COPD Prognostic Score - CPS) using a retrospective analysis of the National Emphysema Treatment Trial cohort. The CPS included: the age mMRC dyspnea scale, post-bronchodilator FEV1 (% predicted), hemoglobin level, respiratory emergency admission rate and daily activity. The CPS ranged from 0 to 16, with 16 indicating the poorest prognosis. A one-point increase in the CPS was associated with an increase,in all-cause death (with a HR of 1.28). The preliminary validation suggests that the CPS (new prognostic scale) had a slightly better prognostic ability than the BODE index and the ADO index. Future validation in a prospective cohort is anticipated.

Omentin-1 levels in patients with premature coronary artery disease, metabolic syndrome and healthy controls. Short communication

David Stejskal, Jan Vaclavik, Ales Smekal, Gabriela Svobodova, Radmila Richterova, Marek Svestak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):219-221 | 10.5507/bp.2016.019

This study showed that patients with premature manifestation of coronary artery disease had significantly lower Omentin-1 serum levels than patients with metabolic syndrome and healthy subjects. To the best of their knowledge, the authors report that this is the first study to assess omentin-1 levels in younger patients with premature manifestations of atherosclerosis. Serum omentin-1 appears to be a useful biomarker of coronary artery disease across the whole age spectrum.

Biodegradable system for drug delivery of hydrolytically labile azanucleoside drugs

Martin Hruby, Khushboo Agrawal, Olivia Policianova, Jiri Brus, Jan Skopal, Pavel Svec, Miroslav Otmar, Petr Dzubak, Petr Stepanek, Marian Hajduch

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):222-230 | 10.5507/bp.2016.013

This study describes a conceptually new system of drug delivery which may increase the plasma circulation time of the hydrolytically labile azanucleoside drugs that are subject to irreversible chemical decomposition in aqueous solution.

Characterization of iron metabolism and erythropoiesis in erythrocyte membrane defects and thalassemia traits

Lucie Sulovska, Dusan Holub, Zuzana Zidova, Martina Divoka, Marian Hajduch, Vladimir Mihal, Jana Vrbkova, Monika Horvathova, Dagmar Pospisilova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):231-237 | 10.5507/bp.2015.054

Pediatric patients with erythrocyte membrane defect and thalassemia trait show disturbed balance between erythropoiesis and iron metabolism. The (hepcidin/ferritin)/sTfR ratio distinguishes patients with erythrocyte membrane defect and the thalassemia trait from healthy populations. These patients should be followed up for possible alterations in erythropoietic activity and potential risk of excessive iron accumulation.

Expression profiles of somatostatin, dopamine, and estrogen receptors in pituitary adenomas determined by means of synthetic multilocus calibrators

Monika Drastikova, Martin Beranek, Filip Gabalec, David Netuka, Vaclav Masopust, Tomas Cesak, Josef Marek, Vladimir Palicka, Jan Cap

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):238-243 | 10.5507/bp.2015.058

This is an investigation on the development of a multilocus calibration material for analysis of somatostatin (SSTR1-5), estrogen (ER1), and dopamine (D2R) receptors in pituitary adenomas. Using this calibrator the authors were able to align all calibration functions, including assays for the transcripts whose expression in the pituitary was very low (SSTR4 and SSTR5). As a result the authors investigated a large group of 206 pituitary tumors with various histo-pathological classification, and defined individual expression profile for postoperative pharmacological treatment with somatostatin analogues and dopamine agonists. The numbers of D2R, ER1, and SSTR1 transcripts were highest in patients with Cushing disease and prolactinomas. SSTR2 expression was significantly higher in acromegaly and thyrotroph adenomas. Significantly more SSTR3 transcripts were found in clinically non-functioning adenomas and acromegaly, and higher levels of SSTR5 were found in patients with acromegaly and prolactinomas.

Serum drug levels to diagnose non-adherence in acute decompensated heart failure

Miroslav Solar, Radek Pelouch, Viktor Vorisek, Vera Furmanova, Jiri Ceral

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):244-247 | 10.5507/bp.2016.031

The estimation of serum drug levels enables diagnosis of non-adherence to recommended therapy. The data suggest that non-adherence is a common problem among patients presenting with acute decompensation of chronic heart failure.

Factors underlying elevated troponin I levels following pacemaker primo-implantation

Tomas Hnatek, Milos Taborsky, Martin Maly, Libor Kamenik, Simona Littnerova, Pavel Sedlon, Jana Luxova, Marta Fiserova, Lenka Pospisilova, Sarka Hamouzova, Josef Danek, Miroslav Zavoral

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):248-256 | 10.5507/bp.2015.067

Pacemaker implantation damages the small area of the myocardium that comes into contact with the electrode tip. This situation leads to slight increases in troponin I levels, resulting in the loss of troponin I's diagnostic role in patients presenting with chest pain shortly after the procedure. Because ECGs can also be influenced during pacing, a diagnostic coronarography is often necessary.

Ten-year survival and prognostic markers in one thousand patients with advanced heart failure. A single-centre analysis

Peter Lesny, Milan Luknar, Martin Matejka, Ivan Varga, Peter Solik, Sona Wimmerova, Eva Goncalvesova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):257-262 | 10.5507/bp.2015.049

The authors describe long-term follow-up of a large, selected and homogenous cohort of patients from a single centre reflecting the course of heart failure with severe left ventricular systolic dysfunction barely modified by comorbidities. Short-term prognosis of heart failure patients could be estimated based on simple parameters available on out-patient examination. Patients presenting with these parameters should be referred to a tertiary centre to be considered for advanced heart failure therapy.

Off-pump versus on-pump coronary artery bypass grafting surgery in high-risk patients: PRAGUE-6 trial at 30 days and 1 year

Jan Hlavicka, Zbynek Straka, Stepan Jelinek, Petr Budera, Tomas Vanek, Marek Maly, Petr Widimsky

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):263-270 | 10.5507/bp.2015.059

In this prospective randomized clinical study PRAGUE-6, the authors collated data from a rarely observed population of high-risk cand idates for direct myocardial revascularisation, an expected early mortality of about 10%. They confirmed that revascuralisation without the use of extracorporal circulation was associated with lower incidence of early major postoperative complications in this subgroup of patients.

Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease

Ivana Rybickova, Viera Vesela, Ivan Fales, Pavlina Skalicka, Katerina Jirsova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):271-275 | 10.5507/bp.2016.001

Ivacaftor in cystic fibrosis adults: Czech experience with six years of follow-up

Libor Fila, Lucie Valentova Bartakova, Alzbeta Grandcourtova, Miloslav Marel, Radovan Drnek, Alena Bilkova, Milan Macek Jr., Pavel Drevinek

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):276-279 | 10.5507/bp.2016.029

The results of this study showed the sustained efficiency of ivacaftor on pulmonary function for treatment periods up to six years, with marked reduction in antibiotic use and hospital stays.

Long-term survival after resections for pancreatic ductal adenocarcinoma. Single centre study

Martin Lovecek, Pavel Skalicky, Dusan Klos, Linda Bebarova, Cestmir Neoral, Jiri Ehrmann Jr., Jana Zapletalova, Hana Svebisova, Radek Vrba, Martin Stasek, Tharani Yogeswara, Roman Havlik

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):280-286 | 10.5507/bp.2016.011

A unique study within the Czech Republic on the long-term survival of patients with resectable pancreatic cancer in a tertiary centre. It demonstrates the effect of proven histopathological vascular invasion on long-term survival in resectable tumors; a factor that is rarely taken into consideration and compares it to other similar international studies. Postoperative complications were another independent prognostic factor after resections.

Patient survival, predictive factors and disease course of severe sepsis in Czech intensive care units: A multicentre, retrospective, observational study

Radovan Uvizl, Milan Adamus, Vladimir Cerny, Ladislav Dusek, Jiri Jarkovsky, Vladimir Sramek, Martin Matejovic, Petr Stourac, Roman Kula, Jan Malaska, Pavel Sevcik

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):287-297 | 10.5507/bp.2015.052

The article presents original epidemiological data on severe sepsis patients in the Czech Republic. It assesses data collected as part of the EPOSS project - the largest database of patients with severe sepsis in Central Europe. It contains information gathered over a period of almost three years. Based on retrospective data analysis, the paper identifies the combination of diagnostic/therapeutic interventions during the initial 6 hours of severe sepsis most closely related to in-hospital mortality. It identifies interventions that may be, based on statistical assessment, considered as risky or protective in relation to in-hospital mortality. In addition, the paper provides data on about the in-hospital morality of patients with severe sepsis with respect to the care procedure and course. It demonstrates the therapy/diagnosis intervention achievement level according to the Sepsis Surviving Campaign.

Clinical manifestations of infective endocarditis in relation to infectious agents: An 8-year retrospective study

Michal Pazdernik, Josef Kautzner, Jan Sochman, Jiri Kettner, Jan Vojacek, Radek Pelouch

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):298-304 | 10.5507/bp.2015.062

This study provides a unique view on IE-related complications in Central Europe. The authors found IE episodes caused by S. aureus were associated with increased short-term and long-term mortality. Of all the studied parameters, only systemic embolism and severe sepsis/septic shock predicted in-hospital mortality. No differences in local structural and/or functional complications (cardiac abscess formation, impaired integrity of the valvular mechanism, conduction disturbances or incidence of heart failure) were observed for S. aureus IE and episodes of IE caused by other aetiological agents.

Prospective comparison of conventional radiography, low-dose computed tomography and magnetic resonance imaging in monoclonal gammopathies

Jiri Minarik, Petra Krhovska, Jan Hrbek, Tomas Pika, Jaroslav Bacovsky, Miroslav Herman, Vlastimil Scudla

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):305-309 | 10.5507/bp.2015.064

This paper confirms the superiority of currently used imaging techniques, low-dose computed tomography (LD-CT) and whole-body magnetic resonance imaging (WB-MRI) over conventional radiography (CR) in assessment of myeloma bone disease (MBD) in a prospectively designed study. The authors conclude that either WB-MRI or LD-CT should be used as preferred imaging techniques in the place of CR in order to detect correctly skeletal involvement in multiple myeloma (MM). Relying solely on CR may underestimate the extent of MBD or even fail to diagnose active MM requiring treatment in a substantial number of patients.

Case reports

A patient with Charlie M Syndrome: Differential diagnosis of Oromandibular Limb Hypogenesis Syndromes

Ole Jung, Ralf Smeets, Henning Hanken, Reinhard E. Friedrich, Max Heiland, Amir Tagniha, Brian Labow

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):310-315 | 10.5507/bp.2016.020

This case report and review gives a brief and critical overview of Oromandibular Limb Hypogenesis Syndromes (OLHS) by analyzing the current state of knowledge. A new, simpler classification of OLHS is introduced.