Biomedical papers, 2016 (vol. 160), issue 2

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(2):271-275 | 10.5507/bp.2016.001

Apoptosis of conjunctival epithelial cells before and after the application of autologous serum eye drops in severe dry eye disease

Ivana Rybickovaa,b, Viera Veselaa, Ivan Falesc, Pavlina Skalickab, Katerina Jirsovaa
a Laboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, General University Hospital and First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
b Department of Ophthalmology, General University Hospital and First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
c Department of Cell Therapy and Cord Blood Bank Czech Republic, Institute of Haematology and Blood Transfusion, Prague, Czech Republic

Aims: To assess the impact of autologous serum eye drops on the level of ocular surface apoptosis in patients with bilateral severe dry eye disease.

Methods: This prospective study was conducted on 10 patients with severe dry eye due to graft versus host disease (group 1) and 6 patients with severe dry eye due to primary Sjögren's syndrome (group 2). Impression cytology specimens from the bulbar conjunctiva were obtained before and after a three-month treatment with 20% autologous serum eye drops applied a maximum of 12 times a day together with regular therapy with artificial tears. The percentage of apoptotic epithelial cells was evaluated immunochemically using anti-active caspase 3 antibody.

Results: In group 1, the mean percentage of apoptotic cells was 3.6% before the treatment. The three-month treatment led to a significant decrease to a mean percentage of 1.8% (P = 0.028). The mean percentage of apoptotic conjunctival cells decreased from 5.4% before the treatment to 3.8% in group 2; however, these results did not reach the level of significance.

Conclusion: Three-month autologous serum treatment led to the improvement of ocular surface apoptosis, especially in the group of patients with severe dry eye due to graft versus host disease. This result supports the very positive effect of autologous serum on the ocular surface in patients suffering from severe dry eye.

Keywords: apoptosis, autologous serum, dry eye disease, caspase 3, conjunctival epithelium

Received: June 15, 2015; Accepted: January 20, 2016; Prepublished online: February 29, 2016; Published: June 24, 2016


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