Biomedical papers, 2019 (vol. 163), issue 1
Reviews
Telomere dynamics in adult hematological malignancies
Helena Olbertova, Karla Plevova, Kamila Stranska, Sarka Pospisilova
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):1-7 | DOI: 10.5507/bp.2018.084 
This is a comprehensive overview of our current knowledge on telomeres and their maintenance in the most common adult hematological malignancies.
Mesenchymal stem cells as the near future of cardiology medicine - truth or wish?
Michaela Brychtova, Jana-Aletta Thiele, Daniel Lysak, Monika Holubova, Milena Kralickova, Lucie Vistejnova
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):8-18 | DOI: 10.5507/bp.2018.071
Mesenchymal Stem Cells are an attractive therapeutic approach in regenerative medicine. This review summarizes the achievements and failures of current in vitro, in vivo and clinical studies that seek to provide evidence for MSC efficacy in cardiology.
Visceral fat and insulin resistance - what we know?
Karolina Janochova, Michal Haluzik, Marek Buzga
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):19-27 | DOI: 10.5507/bp.2018.062
The subject, visceral fat and insulin resistance in obese patients is topical and much published. This review covers current knowledge of the subject complemented by an original table and two pictures that have not been published anywhere. The paragraph "Conclusions and future directions" is also unique because these solutions have not been proposed to date.
Molecular mechanisms of polymyxin resistance and detection of mcr genes
Patrik Mlynarcik, Milan Kolar
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):28-38 | DOI: 10.5507/bp.2018.070
This review provides insight into polymyxin resistance in bacteria with regard to changes in the outer membrane, efflux and other resistance determinants. Better knowledge of the genes involved in polymyxin resistance should pave the way for the development of new and more effective antimicrobial agents. The authors also report novel in silico tested primers for PCR assay that may distinguish colistin-resistant isolates carrying the plasmid-encoded mcr genes.
Original papers
Comparison of a new immunoassay and PCR-based method for quantification of microRNAs in whole blood. A pilot methodical study
David Stejskal, Martina Hlozankova, Radka Sigutova, Katerina Andelova, Zdenek Svagera, Marek Svestak
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):39-44 | DOI: 10.5507/bp.2018.080
MicroRNA determination in blood using the new immunoassay technique (miREIA) produces results that are in very good agreement with those of the RT qPCR kit. Further, the miREIA method is simple and available in most clinical chemistry laboratories.
Increased glutamate and deep brain atrophy can predict the severity of multiple sclerosis
Hubert Polacek, Ema Kantorova, Petra Hnilicova, Marian Grendar, Kamil Zelenak, Egon Kurca
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):45-53 | DOI: 10.5507/bp.2018.036
In multiple sclerosis, deep grey matter (DGM) atrophy is recognised as a crucial component of the disease that presents early and is associated with disability. The authors found that metabolomics of hypothalamus using 1H-Magnetic Resonance spectroscopy (1H-MRS) together with simple planimetry of the third ventricular width at the hypothalamic level (3VW) in T1 weighted MRI pictures reliably detect neurodegeneration of the DGM. The ratio of Glutamate to N-acetylaspartate and 3VW were the only relevant predictors of severity of MS. Glutamate-related 1H-MRS parameters seem to be superior to other metabolites in determining disease burden, independently of otherwise powerful 3VW planimetry.
Comparison of two modes of long-term ECG monitoring to assess the efficacy of catheter ablation for paroxysmal atrial fibrillation
Jan Chovancik, Veronika Bulkova, Dan Wichterle, Ondrej Toman, Lukas Rybka, Jaroslav Januska, Jindrich Spinar, Martin Fiala
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):54-60 | DOI: 10.5507/bp.2018.031
Rationalized cost-effective ECG monitoring that properly assesses the outcome of catheter ablation for paroxysmal atrial fibrillation remains challenging. By comparing two modes of episodic ECG monitoring during 12-month post-ablation follow-up, daily discontinuous ECG recording was found to be superior in detecting recurrent atrial fibrillation compared to continuous episodic ECG monitoring applied twice for around 1 week. Entirely asymptomatic patients with recurrent arrhythmias at risk of misinterpretation of ablation outcome and false decision on further anticoagulation management were rare.
Safety of early discharge in low risk patients after acute ST-segment elevation myocardial infarction, treated with primary percutaneous coronary intervention. Open label, randomized trial
Kamil Novobilsky, Roman Stipal, Petr Cerny, Ivo Horak, Vladimir Kaucak, Jan Mrozek, Jan Vaclavik, Radim Kryza
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):61-66 | DOI: 10.5507/bp.2018.041
The authors present a study comparing the strategy of early and standard discharge in selected patients after myocardial infarction with ST-segment elevation (STEMI). The results show the possibility and safety of a length of hospital stay of 48-72 h and further support the recently released new version of ESC guidelines for STEMI in terms of the level of evidence for the strategy with the shorter recommended length of hospital stay.
Associations between congenital heart defects and genetic and morphological anomalies. The importance of prenatal screening
Jan Pavlicek, Tomas Gruszka, Sabina Kapralova, Martin Prochazka, Eva Silhanova, Romana Kaniova, Slavka Polanska, Renata Cernickova, Eva Klaskova
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):67-74 | DOI: 10.5507/bp.2018.049
Congenital heart defects represent the most frequent congenital defects in the human population, while one third of these cases are associated with further genetic or extra-cardiac morphological anomalies. This association must be carefully taken into consideration in the prenatal counselling.
Intraoperative intraocular pressure fluctuation during standard phacoemulsification in real human patients
Libor Hejsek, Jana Kadlecova, Martin Sin, Vera Velika, Nada Jiraskova
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):75-79 | DOI: 10.5507/bp.2018.065
The aim of this paper was to evaluate the intraocular pressure during the basic phases of standard, real cataract surgery using phacoemulsification. As far as the authors have been able to determine, no study evaluating the fluctuation of the intraocular pressure during standard cataract surgery has yet been published. High values of intraoperative IOL may endanger intraocular perfusion.
25-gauge vitrectomy and gas for the management of rhegmatogenous retinal detachment
Miroslav Veith, Zbynek Stranak, Martin Pencak, Jana Vranova, Pavel Studeny
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):80-84 | DOI: 10.5507/bp.2018.034
Uncomplicated cases of rhegmatogenous retinal detachment can be effectively treated with 25-gauge pars plana vitrectomy.
Case reports
Extraction of a dislocated leadless pacemaker in a patient with infective endocarditis and repeated endocardial and epicardial pacing system infections
Milos Taborsky, Tomas Skala, Martin Kocher, Marian Fedorco
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):85-89 | DOI: 10.5507/bp.2018.020
This a case-report describing a unique complication of a novel invasive method with severe clinical consequences. Retrieval of a dislocated Micra leadless pacemaker is possible thus improving the overall safety of leadless pacemakers.
Therapeutic plasma exchange in secondary prevention of acute pancreatitis in pregnant patient with familial hyperchylomicronemia
Renata Michalova Jr., Anna Mankova, Matej Vnucak, Stanislava Mikulova, Frantisek Nehaj, Katarina Raslova, Ivana Dedinska, Alena Jezikova, Pavel Makovicky, Peter Galajda, Marian Mokan
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(1):90-94 | DOI: 10.5507/bp.2018.044
Therapeutic plasma exchange was successfully used in preventing acute pancreatitis in a 27-year-old pregnant woman with familial hyperchylomicronemia and a history of recurrent hypertriglyceridemia-induced acute pancreatitis associated with pregnancy termination due to death of the fetus, and healthy baby was born.