Biomedical papers, 2020 (vol. 164), issue 3

Reviews

Multiple sclerosis and COVID-19

Jan Mares, Hans-Peter Hartung

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):217-225 | DOI: 10.5507/bp.2020.033  

The article provides an up-to-date overview of the current awareness of a new type of coronavirus. It is introduced with current information on the course of COVID-19 infections. The authors further offer recommendations on how to proceed with possible modification of immunomodulatory therapy in multiple sclerosis in the light of COVID-19 infection, since it is more difficult for physicians to recommend appropriate therapy if a patient develops COVID-19, especially if the patient is also suffering from another disease that may be exacerbated by a new type of coronavirus.

Coagulation disorders in SARS-CoV-2 infection

Romeo Gabriel Mihaila, Marius Dragos Mihaila

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):226-235 | DOI: 10.5507/bp.2020.037  

Hypercoagulability in SARS-CoV-2 infection is part of the complex pathogenesis of the disease. Thrombotic risk quantification and personalized anticoagulant therapy are necessary to avoid unfavorable evolution.

Metabolomic analysis in ophthalmology

Neshe Nazifova-Tasinova, Mladena Radeva, Bistra Galunska, Christina Grupcheva

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):236-246 | DOI: 10.5507/bp.2020.028  

Study of the tear and ocular tissue metabolome may help us understand the metabolism of normal ocular tissues and mechanisms in disease, to discover inter-individual variations within the ocular environment and, thus improve diagnosis and prognosis. Metabolomic profiling of tear, aqueous humor and vitreous samples is useful for identifying biomarkers for more precise diagnosis, prognosis, and therapy.

Original papers

Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations

Katerina Zachova, Petr Kosztyu, Josef Zadrazil, Karel Matousovic, Karel Vondrak, Petr Hubacek, Klara Kostovcikova, Helena Tlaskalova Hogenova, Jiri Mestecky, Milan Raska

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):247-254 | DOI: 10.5507/bp.2019.052  

This investigation characterizes the features of a healthy subject's peripheral blood B lymphocytes infected with Epstein-Barr virus (EBV) using the multi-parametric flow cytometry technique. EBV-infected B cells are predominantly immunoglobulin M (IgM)+ memory or IgA+ plasmablasts. In comparison to uninfected counterparts, the population of EBV-infected B cells is significantly reduced for upper respiratory tract homing α4β1 integrin and enriched for peripheral lymph node targeting CCR7 positive cells, indicating significant deviation in their migratory and maturation profile.

Diagnostic criteria for the determination of clinically significant internal carotid artery stenosis using duplex ultrasound

Karel Gorican, Miroslav Chochola, Miroslav Kocik, Ales Zak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):255-260 | DOI: 10.5507/bp.2019.029  

Diagnostic criteria allow for reliable detection of internal carotid artery stenosis and thus select appropriate therapeutic options.

Atrial fibrillation inducibility after pulmonary vein isolation under general anaesthesia

Tomas Skala, Zbynek Tudos, Ondrej Moravec, Martin Hutyra, Jan Precek, Jitka Skalova, Olga Klementova, Jana Zapletalova, Milos Taborsky

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):261-266 | DOI: 10.5507/bp.2019.004  

Neither AF inducibility or the presence or absence of its early spontaneous termination after pulmonary vein isolation under general anaesthesia in paroxysmal AF patients are useful as predictors of arrhythmia recurrence during 12 months after ablation. The clinical usefulness of rapid atrial stimulation / AF induction in paroxysmal AF patients after pulmonary veins isolation remains questionable.

Associations between CYP2J2 (-76G>T) rs890293 polymorphism and age-related macular degeneration

Rasa Liutkeviciene, Alvita Vilkeviciute, Roman Botov, Olga Botova, Dovile Buteikiene, Loresa Kriauciuniene

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):267-272 | DOI: 10.5507/bp.2019.019  

Age-related macular degeneration (AMD) is the leading cause of blindness in elderly individuals in developed countries. The etiology of AMD is thought to be multifactorial, including environmental and genetic factors. Macular degenerative changes occur due to the formation of drusen, about 40% of which is lipids. As the CYP2J2 gene is involved in the metabolism of lipids, it was selected for investigation in this study. The results revealed that the CYP2J2 (-76G>T) TT genotype may be associated with reduced manifestation of early stage AMD. A larger sample is required for further analysis.

Functional magnetic resonance imaging following epimacular and internal limiting membrane peeling - ipsilateral and contralateral findings

Jan Lestak, Bohdana Kalvodova, Ivan Karel, Jaroslav Tintera

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):273-276 | DOI: 10.5507/bp.2019.044  

Available studies on peeling of the internal limiting membranr (ILM) follow mainly short-term visual outcomes. Our study is unique in following patients for a period of 3-4 years. The results show that peeling of the ILM is damaging to the visual centres of the brain, not only by the surgical intervention but also contralaterally. No similar study has yet been published to the best of our knowledge.

Salivary gland function in thyroid cancer patients with radioiodine administration history

Eva Krcalova, Jiri Horacek, Filip Gabalec, Pavel Zak, Jiri Dolezal

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):277-283 | DOI: 10.5507/bp.2019.023  

In contrast to the parotid glands, submandibular salivary gland uptake and excretion of radioiodine are not decreased even after RAI treatment using the range from 11.10 GBq to 27.75 GBq in comparison to their RAI-naive age- and sex-matched counterparts.

Complications of the surgical treatment of esophageal cancer and microbiological analysis of the respiratory tract

Radek Vrba, Cestmir Neoral, Katherine Vomackova, David Vrana, Bohuslav Melichar, Lucie Lubuska, Yvona Loveckova, Rene Aujesky

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):284-291 | DOI: 10.5507/bp.2019.040  

This is a report on the outcome of surgical treatment of esophageal cancer. Compared are the outcome of the surgery with and without preoperative microbiological sputum examination. In the case of positive sputum findings, the surgery was postponed and antibiotic treatment of the infection was indicated or prophylactic antibiotic therapy was used. In such patients there was a significant decline in severe respiratory tract complications (ARDS, respiratory failure) and the overall mortality was also reduced.

HPV, protein p16 and squamous cell carcinoma of the oral cavity

Jiri Blahak, Jiri Zelinka, Jaromir Gumulec, Ctirad Machacek, Zdenek Danek, Oliver Bulik

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):292-299 | DOI: 10.5507/bp.2019.026  

Carcinoma of the oral cavity is connected with the human papillomavirus. A number of surveys have found that the origin of oropharyngeal cancers is etiologically associated with this high-risk HPV, but in OSCC the presence of HPV is uncommon. Most studies have shown high prevalence of HPV in OPCS (73%), but patients with OSCC have a low infection rate of HPV (4%). Our clinical experience is that there is a marked difference in the prevalence of HPV positivity in OPCS and OSCC patients.

Evaluation and management of toxicity of cytoreductive surgery/hyperthermic intraperitoneal chemotherapy: the initial experience of a single centre study

Radmila Lemstrova, Dominika Flasarova, Martina Spisarova, Bohuslav Melichar, Martin Lovecek, Roman Havlik, Cestmir Neoral, Beatrice Mohelnikova-Duchonova, Dusan Klos

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):300-306 | DOI: 10.5507/bp.2019.035  

This initial experience and preliminary results confirm the safety of the combined treatment modality of CRS/HIPEC although the results are limited by the size and heterogeneity of the cohort. More complications were seen in HIPEC with oxaliplatin in the presented cohort.

In silico study of pseudoprogression in glioblastoma: collaboration of radiologists and radiation oncologists in the estimation of extent of high dose RT region

Renata Belanova, Andrea Sprlakova-Pukova, Michal Standara, Eva Janu, Renata Koukalova, Jan Kristek, Petr Burkon, Ivana Kolouskova, Tomas Prochazka, Petr Pospisil, Arnab Chakravarti, Pavel Slampa, Ondrej Slaby, Tomas Kazda

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):307-313 | DOI: 10.5507/bp.2019.039  

Glioblastoma pseudoprogession is dependent, by definition, on the location within the RT high dose field, however, there is lack of routine reporting of RT isodoses to radiologists during glioma follow up. Using blinded contouring and a number of different indices, we found poor awareness among routine clinical radiologists of per-protocol constructed target volumes for adjuvant glioblastoma RT, thus, automatic tools for exporting of RT target volumes would be very useful for routine clinical practice.

Cancer detection rates and inter-examiner variability of MRI/TRUS fusion targeted biopsy and systematic transrectal biopsy

Miroslav Zalesky, Jiri Stejskal, Ivo Minarik, Vanda Adamcova, Marek Babjuk, Roman Zachoval

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):314-319 | DOI: 10.5507/bp.2019.050  

MRI/TRUS fusion targeted biopsy in combination with systematic biopsy had significantly higher detection rates than systematic biopsy in the rebiopsy group only. Differences in detection rates between examiners are dependent on the level of previous experience with TRUS guided biopsy.

Case report

Pseudoactinomycotic radiate granules in the maxillary sinus. A case report

Ivo Starek, Jaroslav Horacek, Richard Salzman

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(3):320-323 | DOI: 10.5507/bp.2019.012  

Pseudoactinomycotic radiate granules (PAMRAGs) are eosinophilic formations simulating true actinomycotic sulphur granules commonly present in the female genital tract. The PAMRAGs must be taken in the histopathologic differential diagnosis of oesinophilic radiate granules in the maxillary sinus. The presence of surrounding immunoglobulin precipitates the supporting immune mechanism involved in their formation. Their clinical relevance resides in the risk of unnecessary long-term antibiotic treatment if misdiagnosed for actinomycosis.