Biomedical papers, 2017 (vol. 161), issue 4

Review articles

Are we any closer to screening for colorectal cancer using microbial markers?A critical review

Lubomir Stary, Kristyna Mezerova, Pavel Skalicky, Pavel Zboril, Vladislav Raclavsky

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):333-338 | 10.5507/bp.2017.051

Published studies on bacterial species and toxins associated with sporadic colorectal cancer (CRC) are reviewed critically. Bacterial markers supported by conclusive data from independent sources are recommended for further study, including precautions essential to avoid pitfalls and limitations of available methodologies. The need to shift from "overselling of the microbiome" to a more balanced approach that would include conventional culture is emphasized. Regardless of the plethora of open questions, new knowledge that can work in favour of patients already nowadays is summarised as well.

Mechanisms of plasminogen activator inhibitor 1 action in stromal remodeling and related diseases

Jelena Milenkovic, Maja Milojkovic, Tatjana Jevtovic Stoimenov, Boris Djindjic, Edita Miljkovic

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):339-347 | 10.5507/bp.2017.046

Plasminogen activator inhibitor 1 (PAI-1) activity is not only restricted to the inhibition of intravascular fibrinolysis but is also important in a vast number of tissue remodeling processes that involve cell motility and interactions with extracellular matrix. The study summarizes current knowledge and describes in details possible relations of PAI-1 with local humoral and cellular determinants of stromal remodeling during physiological as well as pathological processes (atherosclerosis, tissue fibrosis, pregnancy related complications, cancer metastasis).

Out-of-hospital cardiac arrest

Martin Porzer, Eva Mrazkova, Miroslav Homza, Vladimir Janout

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):348-353 | 10.5507/bp.2017.054

The text presents comprehensive information concerning the issue of out-of-hospital cardiac arrest. The text is summarizing current knowledge of epidemiology of the OHCA and describing the mechanisms of loss of effective circulation. The attention is paid to the circumstances of circulation arrest as well as to relationship between first recorded rhythm and clinical patient outcome. The paper is based particularly on synthesis of large registers' results, dealing with patients resuscitated outside of the hospital environment.

Original contributions

Cloning of intronic sequence within DsRed2 increased the number of cells expressing red fluorescent protein

Rishikaysh V. Pisal, Hana Hrebikova, Jana Chvatalova, Tomas Soukup, Stanislav Filip, Jaroslav Mokry

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):354-359 | 10.5507/bp.2017.033

An artificial intron was cloned within the open reading frame of the DsRed2 gene using cyclic ligation assembly. The successful cloning increased the number of cells expressing red fluorescence by approximately four percent compared to native DsRed2 protein.

Derivation and basic characterization of colorectal carcinoma primary cell lines

Lukas Krbal, Jiri Soukup, Stanislav John, Veronika Hanusova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):360-368 | 10.5507/bp.2017.040

A unique model of primary cancer cell lines isolated from primary colon cancer and respective lymph node metastasis was prepared and tested for basic phenotype properties. Histological and genetic examination of tumour tissue was carried out. The rates of isolation success with histopathological features of the tumour were compared.

Central venous catheter-related thrombosis in intensive care patients - incidence and risk factors: A prospective observational study

Ondrej Hrdy, Eva Strazevska, Petr Suk, Robert Vach, Radim Karlik, Jiri Jarkovsky, Igor Sas, Roman Gal

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):369-373 | 10.5507/bp.2017.034

Insertion of central venous catheter is an integral part of the management of critically ill patients. This study was motivated by the lack of research on CR-DVT. As such, it provides important insights into the factors leading to its development.

The effect of subglottic secretion drainage on the incidence of ventilator associated pneumonia

Marta Walaszek, Agnieszka Gniadek, Malgorzata Kolpa, Zdzislaw Wolak, Alicja Kosiarska

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):374-380 | 10.5507/bp.2017.041

The method of secretion drainage in mechanically ventilated patients may have an influence on the incidence of VAP infections. The risk may be decreased by the use of endotracheal tubes with subglottic secretion drainage. However, regardless of the method of secretion drainage, factors such as reintubation, tracheostomy and bronchoscopy may cause complications including VAP. The most frequent etiological factors responsible for VAP infections were Acinetobacter baumannii.

Analysis of the seasonal incidence of acute respiratory infections including influenza (ARI) in the Czech Republic - possible contribution of the functional data boxplot in epidemiology

Ondrej Vencalek, Jan Kyncl

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):381-388 | 10.5507/bp.2017.042

We propose a new approach for the determination of the baseline incidence level in epidemiology using the latest statistical methods.

Freezing of gait is associated with cortical thinning in mesial frontal cortex

Miroslav Vastik, Pavel Hok, Jan Valosek, Petr Hlustik, Katerina Mensikova, Petr Kanovsky

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):389-396 | 10.5507/bp.2017.035

FreeSurfer detected atrophy of grey matter in Parkinson´s disease with freezing of gait (FOG) in the supplementary motor area, middle/anterior cingulate cortex, temporal pole and right frontal operculum. Development of FOG in patients with PD is therefore associated with local structural cortical changes.

Left hippocampus sparing whole brain radiotherapy (WBRT): A planning study

Tomas Kazda, Miroslav Vrzal, Tomas Prochazka, Petr Dvoracek, Petr Burkon, Petr Pospisil, Adam Dziacky, Tomas Nikl, Radim Jancalek, Pavel Slampa, Radek Lakomy

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):397-402 | 10.5507/bp.2017.031

Only one-sided hippocampal sparing during whole brain radiotherapy translates to further decrease in hippocampal dose in spared (dominant) hemisphere. The clinical significance in terms of better neurocognitive function preservation remains to be tested.

Indications for repositioning of blow-out fractures of the orbital floor based on new objective criteria - tissue protrusion volumometry

Daniel Kovar, Zdenek Voldrich, Pavel Voska, Jan Lestak, Jaromir Astl

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):403-406 | 10.5507/bp.2017.036

The aim of our work was to create a mathematical model to calculate the volume of prolapsed soft tissue of the orbit in blow-out fractures, as a factor in deciding the necessity for surgery.

Endometriosis with an aberrant immunophenotype: Challenging differential diagnosis of glandular lesions in the pelvic lymph nodes

Dita Vlckova, Jiri Lenz, Radek Chvatal, Jan Tihon, Miroslav Kavka, Lucie Uncapher

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):407-412 | 10.5507/bp.2017.032

The finding of pelvic lymph node endometriosis with loss of hormonal receptor expression of the endometrial gland plays a significant role from the pathology standpoint and, perhaps even more importantly, from the clinical standpoint - correct diagnosis of endometriosis saves the patient from treatment for a false positive diagnosis of metastatic adenocarcinoma.

Book reviews

Nadezda Petejova and Arnost Martinek: The Basic Principles of Critical Care Nephrology

Josef Zadrazil

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):v

Milan Halenka and Zdenek Frysak: Atlas of Thyroid Ultrasonography

Ivica Lazurova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(4):vi