Biomedical papers, 2021 (vol. 165), issue 4
Reviews
Cardiac sarcoidosis: from diagnosis to treatment
Kristyna Kysperska, Petr Kuchynka, Tomas Palecek
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):347-359 | DOI: 10.5507/bp.2021.057 
Cardiac sarcoidosis is increasingly recognised as an important cause of life-threatening arrhythmias and heart failure. The aim of this review is to provide readers with current views on the etiopathogenesis, diagnostic and therapeutic options for this condition.
Methods for neovagina creation in women with Mayer-Rokitansky-Küster-Hauser syndrome for subsequent uterus transplantation
Roman Chmel Jr, Zlatko Pastor, Marta Novackova, Nikoleta Chubanovova, Roman Chmel
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):360-366 | DOI: 10.5507/bp.2021.049
This review article focuses on methods of neovagina creation in women with agenesis of the uterus and vagina (Mayer-Rokitansky-Küster-Hauser syndrome) and their suitability for uterus transplantation in the treatment of absolute uterine factor infertility. Because of its noninvasive nature, Frank's or Ingram's dilation methods are preferred first line techniques of neovagina creation and minimally invasive surgical techniques such as Vecchietti's vaginoplasty and skin-graft Abbe-McIndoe's method can be reserved for cases when dilation fails. These methods are viable for the creation of a neovagina in potential candidates for uterus transplantation while intestinal vaginoplasty, Davydov's method and Williams's vaginoplasty do not appear to be so.
Original papers
Alpha-defensins determination in different types of synovial fluid and parallel collected serum samples by ELISA
Pavlina Kusnierova, Iveta Bystronova, Pavel Walder, Rudolf Hlubek, Frantisek Vsiansky, David Stejskal
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):367-374 | DOI: 10.5507/bp.2021.020
Validation of the ELISA diagnostic kit for the determination of alpha-defensin, estimation of physiological limits and correlation with different diagnostic groups suggest the suitability of the biomarker for distinguishing inflammatory from non-inflammatory joint diseases, but unsuitable for rapid decisions in the laboratory.
Diagnostic and prognostic value of placental growth factor serum concentration in clear cell renal cell carcinoma
Marcela Cechova, Matus Chocholaty, Marek Babjuk, Tomas Zima, Klara Havlova, Marketa Koldova, Marek Schmidt, Marta Kalousova
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):375-379 | DOI: 10.5507/bp.2021.003
Placental Growth Factor (PlGF) is crucial in angiogenesis and a potential biomarker in various tumours. The authors evaluated the significance of PlGF serum concentration in clear cell renal cell carcinoma (ccRCC) and based on our finding it seems to be a useful biomarker in diagnostics and prediction of prognosis in patients with ccRCC.
Routine SARS-CoV-2 RT-PCR testing before digestive endoscopy during the peak of the pandemic - a single tertiary center experience
Karolina Novakova, Premysl Falt, Vit Navratil, Matej Halek, Marek Vetesnik, Peter Slodicka, Pavel Sauer, Milan Kolar, Roman Havlik, Jana Zapletalova, Ondrej Urban
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):380-385 | DOI: 10.5507/bp.2021.064
The impact of the COVID-19 pandemic led to suspension of endoscopic care. To maintain the operation of our tertiary unit, we introduced routine RT-PCR testing before endoscopy. During the peak of the pandemic, 1,356 patients were tested at our RT-PCR center and 624 patients were referred with a negative RT-PCR test. There was only a 9% reduction in the number of all procedures compared to the same period before the pandemic outbreak. 44% of procedures revealed new relevant findings and 4.2% newly diagnosed malignancy. Our retrospective analysis shows that routine RT-PCR testing is feasible and can help maintain the productivity of a tertiary endoscopy unit.
Correlation between ADR of screening and all colonoscopies
Ivana Mikoviny Kajzrlikova, Petr Vitek, Josef Chalupa, Jan Kuchar, Jiri Platos, Pavel Reha, Pavel Klvana jr.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):386-389 | DOI: 10.5507/bp.2020.059
Quality of the colonoscopic examination is now considered to be one of the most important part of all colorectal cancer screening programs. For possible gaming with adenoma detection rate for screening colonoscopies, monitorig of adenoma detection rate for all colonoscopies is discussed. The calculation of adenoma detection rate for all colonoscopies was possible in our endoscopic department, and there was a positive correlation with adenoma detection rate for screening colonoscopies. It is a good tool for calculating real numbers from large sample sizes without gaming.
Biopsy of lung lesions under CT control
Miloslav Marel, Radek Padr, Libor Fila, Dmitry Rakita, Fernando Casas Mendez, Linda Capkova, Vaclav Capek, Radim Pavlik
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):390-394 | DOI: 10.5507/bp.2020.040
Thanks to the improvement of diagnostic methods (CT, PET-CT, etc.), an increasing number of patients with radiologically proven pathological foci in the lungs are referred to clinical specialists. The aim of this work was to provide up-to-date information concerning real results of invasive diagnostics and the number of complications to both indicating pneumologists as well as performing radiologists. The authors wanted to determine both the diagnostic benefit of an individual biopsy of lung lesion under CT control (targeted for a radiologist) and the benefit of these procedures for patients (targeted for a pulmonologist). On a model set of 49 "screening like" lung foci authors tried to estimate what can be expected in the near future, after the onset of lung cancer screening with low-dose CT.
Levels of endothelial substances in patients with newly identified hypertension compared with healthy controls
Vladislav Biel, Jan Novak, Veronika Rimalova, Marie Tomandlova, Josef Tomandl, Jiri Spac, Miroslav Soucek
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):395-401 | DOI: 10.5507/bp.2020.062
In this study, the authors sought to find an endothelial substance that has significantly different plasmatic concentration in persons with incipient essential hypertension compared to healthy individuals. This substance has been shown to be nitric oxide (NO), the major endothelial vasodilator. The authors further showed that the plasmatic concentration of NO does not change even after the initiation of intensive antihypertensive treatment and also that endothelial dysfunction cannot be assessed by determining the level of one or more endothelial substances due to very complex interactions of individual substances and the large number of individual modifying factors.
Beta-blockers utilization in heart failure patients: Sub-analysis of a nation-wide population-based study in the Czech Republic
Milos Taborsky, Renata Aiglova, Marie Lazarova, Ludek Pavlu, Josef Danek, Jan Precek, Alexander Schee, Vit Gloger, Marek Vicha, Tomas Skala
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):402-407 | DOI: 10.5507/bp.2020.057
Analysis of all beta-blockers utilization in all patients treated for heart failure in the whole country. The analysis indicates a good translation of current standard of care into common clinical practice based on comparison of drug prescription with specific heart failure registries. Metoprolol remains the mostly prescribed drug. The prescription of bisoprolol and nebivolol increases at the expense of carvedilol.
Post-infarction left ventricular free wall rupture: 12-years experience from the Cardiac Centre of the Institute of Clinical and Experimental Medicine in Prague, Czech Republic
Petr Kacer, Vera Adamkova, Jaroslav Alois Hubacek, Tereza Cervinkova, Vaclav Adamek, Ivana Kralova Lesna, Vera Lanska, Jan Pirk
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):408-415 | DOI: 10.5507/bp.2020.022
A rare diagnosis with high natural and surgical mortality. All currently known surgical techniques have been used for treatment. Mid-term results are presented. The series is one of the largest in the literature.
The number of lymphocytes increases in the periprosthetic tissues with increasing time of implant service in non-metal-on-metal total joint arthroplasties: A role of metallic particles?
Martin Hobza, David Milde, Zuzana Slobodova, Jiri Gallo
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):416-427 | DOI: 10.5507/bp.2020.019
This paper tried to examine an interrelation between low dose metallic content and a lymphocyte-driven response of periprosthetic tissues. Growing concentrations of prosthetic particles in time is associated with non-specific and complex periprosthetic tissue response including areas of lymphocytic infiltrations. However, specific metal-induced changes have not been observed.
Ultrasound cardiac output monitoring in mechanically ventilated children
Jiri Fremuth, Jiri Kobr, Lumir Sasek, Katerina Pizingerova, Jana Zamboryova, Josef Sykora
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):428-434 | DOI: 10.5507/bp.2020.048
Non-invasive ultrasonographic monitoring of cardiac output and hemodynamics for a total of 56 children in the initial phase of mechanical ventilation. Data from patients with pulmonary and extrapulmonary causes of respiratory failure are compared in a clinical prospective observational study. No significant differences were found between the hemodynamic profiles of the two groups of patients.
Estimating the radiation dose to the fetus during prophylactic internal iliac occlusion in patients with abnormal placentation
Marie Cerna, Karel Huml, Martin Kocher, Lenka Jonasova, Tomas Vavra, Vojtech Prasil
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):435-440 | DOI: 10.5507/bp.2020.046
In this manuscript, the authors show that a prophylactic temporary occlusion of internal iliac arteries in patients with abnormal placenta during Caesarean section is a simple and effective method with very low estimated fetal dose.
Optical coherence tomography angiography and the visual field in hypertensive and normotensive glaucoma
Marketa Zakova, Jan Lestak, Martin Fus, Klara Maresova
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):441-444 | DOI: 10.5507/bp.2020.044
The results of this research focused on vessel density, retinal nerve fibre layer and visual field in normotensive and hypertensive glaucoma takes a new view on both diseases. The therapy of normotensive glaucoma should be not based on intraocular pressure lowering, but on better blood circulation in back pole of eye and optic nerve.
Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation
Ming Yan, Ola A. Nada, Ralf Smeets, Martin Gosau, Reinhard E. Friedrich, Lan Kluwe
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):445-451 | DOI: 10.5507/bp.2020.061
To optimize the expansion of human dental pulp cells in vitro while exploring several cryopreservation methodologies. 1 The research is characterized by exploring a new method of cryopreservation dental pulp tissues to establish a bank for the extraction of pulp stem cells. 2 From a practical perspective of clinical stem cell transplantation therapy, tissue cryopreservation rather than cell cryopreservation is put forward as a new concept for pulp stem cells. 3 To identify a suitable long term store of pulp cells for clinical use. To obtain single cells, the corresponding resources and skills are demanding and only available at a limited number of hospitals and clinics. Though many people will likely have some of their teeth extracted during their life, teeth are disposed of as waste whereas they could provide a valuable autologous source for these patients for possible future application.
Case reports
Radial artery pseudoaneurysm as a rare very late complication of transradial cardiac catheterization
Radomir Nykl, Jan Precek, Milos Spacek, Martin Sluka, Stepan Hudec, Petr Heinc, Milos Taborsky
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):452-453 | DOI: 10.5507/bp.2020.027
The authors bring to light the possibility of a rare, very late complication of transradial catheterization. Radial artery pseudoaneurysm formation from this procedure is extremely rare (around 0.05% according to the authors). Such an extremely late development of pseudoaneurysm has not been described in the literature so far.
Novel mutations in TRPM6 gene associated with primary hypomagnesemia with secondary hypocalcemia. Case report
Jan Papez, Jiri Starha, Katerina Slaba, Jaroslav A Hubacek, Jakub Pecl, Stefania Aulicka, Milan Urik, Serdar Ceylaner, Petra Vesela, Ondrej Slaby, Petr Jabandziev
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):454-457 | DOI: 10.5507/bp.2021.027
Primary hypomagnesemia with secondary hypocalcemia is a rare genetic disorder. Dysfunctional transient receptor potential melastatin 6 (TRPM6) causes impaired intestinal absorption of magnesium, leading to its low serum level accompanied by hypocalcemia. Typical signs at initial manifestation are generalized seizures, tetany, and/or muscle spasms. In this paper, we present a clinical report of a girl with primary hypomagnesemia and secondary hypocalcemia due to novel mutations within the TRPM6 gene.