Biomedical papers, 2017 (vol. 161), issue 2

Amyloid cardiomyopathy

Lucie Karafiatova, Tomas Pika

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):117-127 | 10.5507/bp.2017.001

Amyloidosis is a heterogeneous group of diseases characterized by the deposition of amyloid. It is caused by extracellular deposition of insoluble fibrils with beta-pleated sheet configuration. The protein misfolding abnormalities result in amyloid fibrils and may manifest as primary, secondary, or familial amyloidosis. Amyloid deposition can occur in multiple organs (eg, heart, liver, kidney, skin, eyes, lungs, nervous system) resulting in a variety of clinical manifestations. Cardiac involvement is a progressive disorder resulting in early death due to congestive heart failure and arrhythmias. It also can occur as part of a systemic disease or as a localized phenomenon. This article provides an overview of amyloidosis, giving information about diagnosis, treatment and prognosis mainly of amyloid cardiomyopathy.

Use of near-infrared spectroscopy (NIRS) in cerebral tissue oxygenation monitoring in neonates

Rene Gumulak, Lucia Casnocha Lucanova, Mirko Zibolen

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):128-133 | 10.5507/bp.2017.012

This is a review of the mode of function and variability of NIRS-based devices used in neonatology, clinical applications in cerebral monitoring, limitations and disadvantages of the current technology. NIRS is a fascinating and dynamically evolving technology with great potential in NICU, worldwide.

Therapeutic monitoring of amiodarone: pharmacokinetics and evaluation of the relationship between effect and dose/concentration

Erika Hrudikova Vyskocilova, Milan Grundmann, Jana Duricova, Ivana Kacirova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):134-143 | 10.5507/bp.2017.016

This is a review of the pharmacokinetics and monitoring of amiodorone, an important antiarrhythmic drug.

Effects of obesity on liver cytochromes P450 in various animal models

Veronika Tomankova, Pavel Anzenbacher, Eva Anzenbacherova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):144-151 | 10.5507/bp.2017.026

The aim of the paper is to overview the results of animal and/or human studies of the effects of pathophysiological factors, particularly obesity on the properties of individual cytochrome P450 forms.

Polymorphisms at 1q32, 8q24, and 17q22 loci are associated with nonsyndromic cleft lip with or without cleft palate risk in the Slovak population

Jan Salagovic, Lucia Klimcakova, Marianna Zabavnikova, Jana Behunova, Terezia Hudakova, Jozef Fedeles, Agata Molnarova, Ludmila Podracka

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):152-157 | 10.5507/bp.2017.009

Genome-wide association (GWAS) and candidate gene studies have identified several genetic risk loci associated with nonsyndromic cleft lip with or without cleft palate (nsCL/P) but only a few have shown consistent replication. Further studies are needed to replicate GWAS results to identify population-specific nsCL/P loci. In this study, the authors conducted an association analysis of the four well established polymorphisms for nsCL/P in a Slovak population. Three of the four loci revealed significant associations using different genetic models.

Screening for mutations in two exons of FANCG gene in Pakistani population

Ujala Aymun, Saima Iram, Iram Aftab, Saba Khaliq, Nadir Ali, Nisar Ahmed, Shahida Mohsin

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):158-163 | 10.5507/bp.2017.030

This work was done to study the phenotypic spectrum of Fanconi anemia along with the gene mutations in two of the exons of FANCG gene in Pakistan. Fanconi anemia is a rare genetic disorder, but a leading cause of inherited aplastic anemia. It has a vast genotypic and phenotypic spectrum. This study is a small contribution to the existing pool of information about this disease in Pakistan.

Inflammatory, anti-inflammatory and regulatory cytokines in relatively healthy lung tissue as an essential part of the local immune system

Zane Vitenberga, Mara Pilmane

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):164-173 | 10.5507/bp.2017.029

Apart from immune cells, non-immune cells that penetrate deep structures in lung bronchi possess immunogenic properties defined by the specificity of cytokine contents. If not affected by any biological, physiological or chemical triggers, lung tissue maintains a mostly anti-inflammatory environment.

Possitive correlation between adipocyte fatty acid-binding protein and epicardial fat in patients with a family history of cardiovascular disesase

Eliska Sovova, Milan Sova, Jana Zapletalova, David Stejskal, Marketa Sovova, Marketa Kaletova, Gabriela Svobodova, Irena Kuca, Michal Janak, Milan Kaminek

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):174-178 | 10.5507/bp.2017.018

This study focused on positive correlations between serum A-FABP level and epicardial fat. The positive relation found highlights the possible use of this important biomarker of cardiovascular disease.

Avidity of antineurocytoskeletal antibodies in Alzheimer's disease patients

Libuse Noskova, Lenka Fialova, Ales Bartos, Tomas Zima

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):179-186 | 10.5507/bp.2017.017

An optimised ELISA method for the determination of anti-neurofilament heavy subunit antibody avidity is suitable for research on anti-NfH antibody avidity in patients with neurological diseases associated with neurocytoskeletal defects. However, the results showed no difference in levels or avidity of IgG anti-NfH antibodies in Alzheimer´s disease patients and cognitively normal persons.

Incidence and risk factors for delirium development in ICU patients - a prospective observational study

Marcela Kanova, Peter Sklienka, Roman Kula, Michal Burda, Jana Janoutova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):187-196 | 10.5507/bp.2017.004

Delirium and brain dysfunction, is frequent complication in critically ill patients . The primary aim of this study was to recognize and explore risk factors of the development delirium to allow prevention. The main risk factors are age, APACHE II score, admission type, alcohol abuse in anamnesis, length of ICU stay, duration of artificial ventilation, need for vasopressors, and the use of drug-induced sedation.

Clonality testing of lymphoproliferative disorders in a large cohort of primary and consultant biopsies

Michaela Svachova, Martin Tichy, Patrik Flodr, Jana Steigerova, Zdenek Kolar, Jan Bouchal

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):197-205 | 10.5507/bp.2017.006

A large cohort of nearly 1,000 formalin-fixed paraffin embedded tissues was tested by molecular methods for the presence of clonality of IgH and TCR in lymphoid neoplasms. The importance of the PCR analysis of IgH and TCR gene rearrangements is further illustrated by ten selected case reports.

Scharioth Macula Lens: A new intraocular implant for low-vision patients with stabilized maculopathy- first experience

Jana Nekolova, Pavel Rozsival, Martin Sin, Nada Jiraskova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):206-209 | 10.5507/bp.2017.014

This article is about the Scharioth Macula Lens implant which acts as a magnifier in the eye and improves near vision in low vision patients with stabilized maculopathy. The first experience with this intraocular lens is presented.In the experience of the authors, the prospects of reading rehabilitation after SML implantation are promising; despite very severe findings in the macula, the SM and enabled the patients involved to read again and with a better quality of life.

Methylation status as a predictor of intravesical Bacillus Calmette-Guérin (BCG) immunotherapy response of high grade non-muscle invasive bladder tumor

Petr Husek, Jaroslav Pacovsky, Marcela Chmelarova, Miroslav Podhola, Milos Brodak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):210-216 | 10.5507/bp.2017.008

This paper offers an inovative view on epigenetic analysis in clinical practice. Methylation analysis is unique for parafin fixed samples. The results have promising clinical impact.

Locally advanced breast cancer in elderly patients

Jana Hornova, Zbynek Bortlicek, Petra Majkova, Jitka Abrahamova, Drahomira Kordikova, Zuzana Donatova, Martin Kupec, Ludmila Boublikova, Josef Dvorak, Radek Zapletal, Tomas Buchler

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017, 161(2):217-222 | 10.5507/bp.2017.011

Locally advanced breast cancer (LABC) is relatively common in the elderly population. The authors studied the clinical characteristics and survival of patients aged 70 years or more with LABC. Age and severe comorbidities measured using the Charlson score were significantly associated with adverse overall survival in these patients.