Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2021, 165(4):445-451 | DOI: 10.5507/bp.2020.061

Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation

Ming Yan, Ola A. Nada, Ralf Smeets, Martin Gosau, Reinhard E. Friedrich, Lan Kluwe
Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg 20246, Germany

Background and Aims: Teeth extracted are usually disposed as bio-waste whereas they could serve as an autologous tissue for culturing multipotent dental pulp cells which have application potential in regenerative medicine. This study aimed to examine the feasibility of cryopreserving dental pulp tissue at teeth extraction for later culturing of cells.

Methods: The pulp tissue from each of a total of 10 teeth was cut into small fragments which were then divided into two portions. One portion was directly used for culturing pulp cells using the explant method. The other portion was cryopreserved with 10% DMSO in liquid nitrogen for at least one month and then thawed for culturing pulp cells.

Results: Vital cells were obtained from all the 10 pulp fragment suspensions which went through cryopreservation. The cell outgrowth from the explants of cryopreserved pulp fragments was two days later than that of corresponding fresh pulp tissue. Otherwise, no difference was observed in proliferation, expression of stem cell markers and differentiation into adipose cells and osteoblasts between the two groups of cells cultured from the fresh or the cryopreserved pulp fragments.

Conclusions: Cryopreserving fragmented dental pulp tissue provides a feasible option for saving pulp tissues as autologous cell sources for possible later application.

Keywords: dental pulp cells, differentiation, dental pulp tissue, cell culture, tissue cryopreservation, osteogenic differentiation, adipogenic differentiation

Received: May 6, 2020; Revised: September 25, 2020; Accepted: November 27, 2020; Prepublished online: December 15, 2020; Published: November 23, 2021  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Yan, M., Nada, O.A., Smeets, R., Gosau, M., Friedrich, R.E., & Kluwe, L. (2021). Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation. Biomedical papers, 165(4), 445-451. doi: 10.5507/bp.2020.061
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A 2000;97(25):13625-30. Go to original source... Go to PubMed...
    2. Aydin S, Sahin F. Stem Cells Derived from Dental Tissues. Adv Exp Med Biol 2019;1144:123-32. Go to original source... Go to PubMed...
    3. Dehghani Nazhvani A, Ahzan S, Hosseini SM, Attar A, Monabati A, Tavangar MS. Purification of Stem Cells from Oral Pyogenic Granuloma Tissue. Open Dent J 2018;12:560-66. Go to original source... Go to PubMed...
    4. Wang Z, Pan J, Wright JT, Bencharit S, Zhang S, Everett ET, Teixeira FB, Preisser JS. Putative stem cells in human dental pulp with irreversible pulpitis: an exploratory study. J Endod 2017;36(5):820-25. Go to original source... Go to PubMed...
    5. Chalisserry EP, Nam SY, Park SH, Anil S. Therapeutic potential of dental stem cells. J Tissue Eng 2017;8:2041731417702531. Go to original source... Go to PubMed...
    6. Young F, Sloan A, Song B. Dental pulp stem cells and their potential roles in central nervous system regeneration and repair. J Neurosci Res 2013;91(11):1383-93. Go to original source... Go to PubMed...
    7. Fabricant L, Kiraly L, Wiles C, Differding J, Underwood S, Deloughery T, Schreiber M. Cryopreserved deglycerolized blood is safe and achieves superior tissue oxygenation compared with refrigerated red blood cells: a prospective randomized pilot study. J Trauma Acute Care Surg 2013;74(2):371-76; discussion 376-377. Go to original source... Go to PubMed...
    8. Xu X, Cowley S, Flaim CJ, James W, Seymour LW, Cui Z. Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog 2010;26(3):781-88. Go to original source... Go to PubMed...
    9. Zanata F, Bowles A, Frazier T, Curley J, Bunnell BA, Wu X, Wade J, Devireddy R, Gimble JM, Ferreira LM. Effect of Cryopreservation on Human Adipose Tissue and Isolated Stromal Vascular Fraction Cells: In Vitro and In Vivo Analyses. Plast Reconstr Surg 2018;141(2):232e-243e. Go to original source... Go to PubMed...
    10. Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A, DenBesten P, Gehron R, Shi S. Stem cell properties of human dental pulp stem cells. J Dent Res 2002;81(8):531-35. Go to original source... Go to PubMed...
    11. Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, Kortesidis A, Simmons PJ. Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J Cell Sci 2003;116(Pt 9):1827-35. Go to original source... Go to PubMed...
    12. Gu K, Smoke RH, Rutherford RB. Expression of genes for bone morphogenetic proteins and receptors in human dental pulp. Arch Oral Biol 1996;41(10):919-23. Go to original source... Go to PubMed...
    13. Kerkis I, Kerkis A, Dozortsev D, Stukart-Parsons GC, Massironi SM, Pereira LV, Caplan AI, Cerruti HF. Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. Cells Tissues Organs 2006;184(3-4):105-16. Go to original source... Go to PubMed...
    14. Miura M, Gronthos S, Zhao M. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U S A 2003;100(10):5807-12. Go to original source... Go to PubMed...
    15. Alongi DJ, Yamaza T, Song Y, Fouad AF, Romberg EE, Shi S, Tuan RS, Huang GT. Stem/progenitor cells from inflamed human dental pulp retain tissue regeneration potential. Regen Med 2010;5(4):617-31. Go to original source... Go to PubMed...
    16. Thirumala S, Goebel WS, Woods EJ. Clinical grade adult stem cell banking. Organogenesis 2009;5(3):143-54. Go to original source... Go to PubMed...
    17. Munevar JC, Gutierrez N, Jimenez NT, Lafaurie GI. Evaluation of two human dental pulp stem cell cryopreservation methods. Acta Odontol Latinoam 2015;28(2):114-21. Go to PubMed...
    18. Takebe Y, Tatehara S, Fukushima T, Tokuyama-Toda R, Yasuhara R, Mishima K, Satomura K. Cryopreservation Method for the Effective Collection of Dental Pulp Stem Cells. Tissue Eng Part C Methods 2017;23(5):251-61. Go to original source... Go to PubMed...
    19. Huynh NC, Le SH, Doan VN, Ngo LTQ, Tran HLB. Simplified conditions for storing and cryopreservation of dental pulp stem cells. Arch Oral Biol 2017;84:74-81. Go to original source... Go to PubMed...
    20. Alsulaimani RS, Ajlan SA, Aldahmash AM, Alnabaheen MS, Ashri NY. Isolation of dental pulp stem cells from a single donor and characterization of their ability to differentiate after 2 years of cryopreservation. Saudi Med J 2016;37(5):551-60. Go to original source... Go to PubMed...
    21. Perry BC, Zhou D, Wu X, Yang F, Byers MA, Chu TG, Hockema JJ, Woods EJ, Goebel WS. Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use. Tissue Eng Part C Methods 2008;14(2):149-56. Go to original source... Go to PubMed...
    22. Collart Dutilleul PY, Thonat C, Jacquemart P, Cuisinier F, Levallois B, Chaubron F. Dental pulp stem cells: characteristics, cryopreservation and therapeutic potentialities. Orthod Fr 2012;83(3):209-16. Go to original source... Go to PubMed...
    23. Balci D, Can A. The assessment of cryopreservation conditions for human umbilical cord stroma-derived mesenchymal stem cells towards a potential use for stem cell banking. Curr Stem Cell Res Ther 2013;8(1):60-72. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content