Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(1):056-064 | DOI: 10.5507/bp.2012.031

Array-based karyotyping in chronic lymphocytic leukemia (CLL) detects new unbalanced abnormalities that escape conventional cytogenetics and CLL FISH panel

Helena Urbankova, Tomas Papajik, Radek Plachy, Milena Holzerova, Jana Balcarkova, Martina Divoka, Vit Prochazka, Zuzana Pikalova, Karel Indrak, Marie Jarosova
Department of Hemato-oncology, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic

Aims: Chronic lymphocytic leukemia (CLL) is the most common adult leukemia with a very heterogeneous course. Progress in molecular genetic characterization of CLL has confirmed the prognostic role of unbalanced chromosomal abnormalities currently defined by molecular cytogenetic methods: conventional karyotyping and FISH. However, a significant percentage of genomic abnormalities escapes routine investigation due to the limitations of these methods. It is presently clear that some of these aberrations have impact on prognosis and disease progression.

Methods: We examined copy number changes in the tumor genomes of 50 CLL patients using bacterial artificial chromosome (BAC) and/or oligonucleotide array platforms.

We compared the results of arrayCGH with those obtained by FISH and conventional cytogenetics and evaluated their clinical importance.

Results: A total of 111 copy number changes were detected in 43 patients (86%) with clonal abnormalities present in at least 23% of the cells. Moreover, 14 patients (28%) were found to have 39 genomic changes that had not been detected by standard cytogenetic and/or FISH analyses. These included possibly prognostically important recurrent 2p and 8q24 gains. The most frequent unbalanced changes involved chromosomes 18, 7, 3, 9 and 17. We also determined the minimal deleted region on chromosome 6q in 7 cases by chromosome 6/7 specific array.

Conclusions: The results showed that a subset of potentially significant genomic aberrations in CLL is being missed by the current routine techniques. Further, we clearly demonstrated the robustness, high sensitivity and specificity of the arrayCGH analysis as well as its potential for use in routine screening of CLL.

Keywords: chronic lymphocytic leukemia, cancer, arrayCGH, copy number changes, chromosomal abnormalities, del(6)(q)

Received: November 8, 2011; Accepted: February 27, 2012; Prepublished online: June 1, 2012; Published: April 1, 2014  Show citation

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Urbankova, H., Papajik, T., Plachy, R., Holzerova, M., Balcarkova, J., Divoka, M., ... Jarosova, M. (2014). Array-based karyotyping in chronic lymphocytic leukemia (CLL) detects new unbalanced abnormalities that escape conventional cytogenetics and CLL FISH panel. Biomedical papers, 158(1), 056-064. doi: 10.5507/bp.2012.031
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