Biomedical papers, 2015 (vol. 159), issue 2

Editorial

The juice of your paper

Jan Bouchal

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):

Review articles

Hypoxia-induced chemoresistance in cancer cells: The role of not only HIF-1

Helena Doktorova, Jan Hrabeta, Mohamed Ashraf Khalil, Tomas Eckschlager

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):166-177 | 10.5507/bp.2015.025

Adaptation to hypoxia requires many genetic and biochemical responses that mutually regulate one another. Hypoxia-induced resistance is consequently very complex. The most studied factor responsible is hypoxia-inducible factor-1 (HIF-1) that significantly contributes to the aggressiveness and chemoresistance of various tumours. Also described are HIF-1 independent mechanisms of drug resistance in hypoxic tumours. Since hypoxia is closely associated with chemo- and radio resistance, different approaches have been developed to circumvent hypoxia-induced resistance.

An overview of the risk factors associated with multiple oral premalignant lesions with a case report of extensive field cancerization in a female patient

Gadiputi Sreedhar, Masineni Narayanappa Sumalatha, Deepika Shukla

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):178-183 | 10.5507/bp.2013.092

A review of the the literature has revealed the presence of a field with genetically altered cells that appear to be induced by tobacco (smoking/smokeless form). A large number of premalignant cells in the fields, may increase cancer risk.

The miR-29 family in hematological malignancies

Barbora Fiserova, Lenka Kubiczkova, Lenka Sedlarikova, Roman Hajek, Sabina Sevcikova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):184-191 | 10.5507/bp.2014.037

Two sides of the miRNA29 family. Are they suppressors or promoters in hematooncology?

The role of tissue factor in normal pregnancy and in the development of preeclampsia: A review

Jana Prochazkova, Ludek Slavik, Jana Ulehlova, Martin Prochazka

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):192-196 | 10.5507/bp.2014.061

This paper summarises recent evidence on of the structure and functions of tissue factor. It covers the role of tissue factor and its inhibitor in blood coagulation, the inflammatory response, angiogenesis and cell migration. The paper is specifically aimed at the role of tissue factor in the uteroplacental vessel network during physiological pregnancy and pregnancy complicated by preeclampsia

Pathophysiology of anemia in chronic kidney diseases: A review

Josef Zadrazil, Pavel Horak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):197-202 | 10.5507/bp.2013.093

This paper focuses on current knowledge of the pathophysiology of anemia in CKD. It provides details on the physiology and pathophysiology of erythropoietin and iron metabolism in CKD and briefly discusses other known factors which may be important for the development of renal anemia. Understanding the pathophysiology of anemia in CKD is crucial for optimal management of anemia. Changes in treating anemia in patients with CKD which have created new recomendations (KDIGO, 2012) to the American (KDOQI, 2006) and European (ERBP, 2004) are summarized.

Current treatment methods for long occlusions of the femoropopliteal segment in patients with intermittent claudication: Minireview

Jana Fialova, Petr Utikal, Petr Bachleda, Martin Kocher, Marie Cerna, Katherine Vomackova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):203-207 | 10.5507/bp.2013.018

Intermittent claudication in patients with long occlusion of the femoropopliteal segment is mainly treated conservatively but if this fails, a form of revascularization is indicated. The revascularization is currently achieved using two basic methods : surgical bypass or mini invasively by endovascular procedures. The authors compare the two approaches in order to to answer the question whether surgical bypass is still the best method of choice in an era of endovascular techniques.

The common pathophysiology underlying the metabolic syndrome, schizophrenia and depression. A review

Jana Kucerova, Zuzana Babinska, Katerina Horska, Hana Kotolova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):208-214 | 10.5507/bp.2014.060

There is large body of evidence of metabolic syndrome development as a consequence of antipsychotic treatment in schizophrenic patients. However, recently the schizophrenic phenotype per se was proven to contribute to the metabolic distortions. Similarly, this connection has been reported in depressed patients. This review maps inflammatory pathology as a common underlying cause of metabolic syndrome and psychiatric comorbidities. The paper also assesses possible gender differences.

Original contributions

In vitro testing of immunosupressive effects of mesenchymal stromal cells on lymphocytes stimulated with alloantigens

Daniel Lysak, Tomas Vlas, Monika Holubova, Michaela Miklikova, Pavel Jindra

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):215-219 | 10.5507/bp.2013.072

Mesenchymal stromal cells (MSC) exhibit an immunomodulatory potential in patients with graft versus host disease following allogeneic hematopoietic stem cell transplantation. The immunosuppressive function of MSC was examined by coculturing them with stimulated HLA incompatible allogeneic lymphocytes in a mixed lymphocyte culture test. The presence of MSC caused significant decrease in the metabolic and proliferative activity of lymphocytes. The absorbance levels determined with MTT test decreased by 62% (P<0.01), 26% (P<0.01) and 6% (P=0.0437) in comparison to positive controls depending on the MSC/lymphocyte ratio (1:5, 1:50, 1:500). The experiments confirmed the dose-dependent inhibitory effect of MSC on lymphocyte proliferation triggered by stimulation with alloantigens.

Anti-edema effect of melatonin on spinal cord injury in rats

Xinwei Liu, Yu Wang, Jishun Yang, Yunen Liu, Dapeng Zhou, Mingxiao Hou, Liangbi Xiang

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):220-226 | 10.5507/bp.2015.012

Melatonin can reduce the expressions of AQP4 and GFAP in rat spinal cord tissue and inhibit edema after spinal cord injury. Melatonin provides fairly good protection and treatment for spinal cord injury in rats.

Biocompatibility of quantum dots (CdSe/ZnS ) in human amniotic membrane-derived mesenchymal stem cells in vitro

Gongping Wang, Guangwei Zeng, Caie Wang, Huasheng Wang, Bo Yang, Fangxia Guan, Dongpeng Li, Xiaoshan Feng

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):227-233 | 10.5507/bp.2014.045

This paper describes research based on quantum dots between human amniotic membrane mesenchymal stem cells. The observed compatibility of the two and the changes in cell growth, provide a new approach to cell markers

Predictive value of systemic and local inflammation parameters in talc pleurodesis assessment

Petr Habal, Nedal Omran, Karolina Jankovicova, Jan Krejsek, Jiri Mandak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):234-241 | 10.5507/bp.2014.038

This paper describes a palliative treatment of recurrent malignant pleural effusion by powdered talc using thoracoscopy. The talc- induced pleurodesis is a result of an inflammatory reaction in both pleural membranes. The paper presents the original results of selected systemic and local manifestations of the talc-induced inflammatory reaction (serum and pleural effusion CRP levels, local expression of membrane receptors TLR-2 and CD-64 on granulocyte and monocyte cell populations in the pleural effusion).

The effect of Helicobacter pylori eradication on insulin resistance and HbA1c level in people with normal glucose levels: a prospective study

Zeynal Dogan, Murat Sarikaya, Bilal Ergul, Levent Filik

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):242-245 | 10.5507/bp.2014.036

Helicobacter pylori (H. pylori) infection is a common health problem worlwide. Eradication of H. pylori results in lower rates of complications related to peptic ulcer disease. In this study, eradication of H. pylori was found to have favorable effects on insulin resistance and HbA1c levels in people with normal glucose levels.

Effect of renal denervation on glucose metabolism after a 12 month follow-up

Daniel Matous, Otakar Jiravsky, Igor Nykl, Marian Branny,

Editorial Commentary, p. 338

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):246-250 | 10.5507/bp.2015.030

In this study, the authors provide their own data of the currently controversial renal denervation procedure: recent results from Symplicity HTN-3 showed no statistically significant difference between renal denervation and sham procedure in blood pressure lowering. The data do not confirm any other other positive effect of renal denervation for treating insulin resistance/diabetes mellitus 2. type. To date, there is no recommendation for the use of renal denervation in the treatment of resistant hypertension in routine clinical practice.

Unstable angina pectoris prior to ST elevation myocardial infarction in patients treated with primary percutaneous coronary intervention has no influence on prognosis

Krystyna Kluz, Jiri Parenica, Lenka Kubkova, Simona Littnerova, Josef Tomandl, Martin Poloczek, Ondrej Toman, Martin Tesak, Zdenka Cermakova, Jana Gottwaldova, Jan Manousek, Monika Pavkova Goldbergova, Jindrich Spinar, Jiri Jarkovsky

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):251-258 | 10.5507/bp.2014.003

Pre-infarction unstable angina pectoris (UAP) can be considered ischemic preconditioning. The aim of this study was to compare short and long term outcomes in patients (N=593) with (N=205) or without pre-infarction UAP (N=388) and ST elevation myocardial infarction (STEMI) treated with primary percutaneous coronary intervention (PCI). In patients with STEMI treated with primary PCI, pre-infarction UAP has no beneficial clinical effect during hospitalization or during long-term follow-up.

Assessment of the severity of acute pulmonary embolism using CT pulmonary angiography parameters

Vladimir Kincl, Vera Feitova, Roman Panovsky, Radka Stepanova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):259-265 | 10.5507/bp.2013.082

The aim of this study was to assess the clinical benefit of several parameters measured from CT pulmonary angiography. Their relation to clinical outcome - administration of thrombolytic therapy and to other currently used methods (biomarkers,echocardiography) were studied. The statistical analysis, showed the RV/LV diameter ratio measured from CT images was the most valid parameter.

Eosinophil count is related with coronary thrombus in non ST-elevated acute coronary syndrome

Erhan Tenekecioglu, Mustafa Yilmaz, Adem Bekler, Serafettin Demir

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):266-271 | 10.5507/bp.2014.039

This article describes the role of eosinophils in predicting the formation of thrombus in non ST-segment elevated myocardial infarction (NSTEMI). Thrombus formation has frequently been reported as the most crucial contributing factor in ST-segment elevated MI. In this study the authors show that this crucial factor should not be disregarded in NSTEMI patients either.

Predictive value of serum collagen biomakers on the outcome of acute myocardial infarction treated with percutaneous coronary intervention

Hang Gao, Xue-Song Zhang, Qiang Zhao, Long-Le Ma, Le-Xin Wang

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):272-276 | 10.5507/bp.2013.091

This prospective clinical study showed that in patients who were treated with percutaneous coronary intervention following ST elevation myocardial infarction, serum levels of procollagen type I carboxyterminal peptide (PICP) and procollagen type III peptide (PIIINP) are predictive of outcome within 12 months of treatment.

The effect of continuous positive airway pressure therapy on the prevalence of masked hypertension in obstructive sleep apnea patients

Milan Sova, Eliska Sovova, Milada Hobzova, Jana Zapletalova, Monika Kamasova, Vitezslav Kolek

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):277-282 | 10.5507/bp.2014.063

This was a study of the effect of continuous positive airway pressure (CPAP) on the prevalence of masked hypertension in Obstructive Sleep Apnea patients. Masked hypertension is very prevalent in these patients and CPAP could be effective in blood pressure lowering. However this study nopositive effect of CPAP on the prevalence of masked hypertension was found

Paroxysmal atrial fibrillation in young cryptogenic ischemic stroke: A 3-week ECG Holter monitoring study

Daniel Sanak, Martin Hutyra, Michal Kral, Andrea Bartkova, Jana Zapletalova, Marian Fedorco, Tomas Veverka, David Vindis, Tomas Dornak, Tomas Skala, David Skoloudik, Milos Taborsky, Petr Kanovsky

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):283-287 | 10.5507/bp.2015.019

The cause of cryptogenic ischemic stroke in younger patients often remains obscure- -. In elderly patients with cryptogenic stroke, paroxysmal atrial fibrillation (AF) is a frequent cause of stroke. Thus, undetected paroxysmal AF is considered a possible cause in young patients as well. However insufficient relevant data on the incidence of paroxysmal AF are available in this stroke population. This article reports the rate of detected paroxysmal AF in young cryptogenic ischemic stroke patients ≤ 50 years using long-term ECG Holter monitoring.

Extramedullary relapse of multiple myeloma defined as the highest risk group based on deregulated gene expression data

Sabina Sevcikova, Helena Paszekova, Lenka Besse, Lenka Sedlarikova, Veronika Kubaczkova, Martina Almasi, Ludek Pour, Roman Hajek

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):288-293 | 10.5507/bp.2015.014

Extramedullary relapse of multiple myeloma is slowly becoming a new challenge in the treatment of multiple myeloma patients. It appears that extramedullary relapse may be characterized by a clone of plasma cells that accumulate more genomic abnormalities allowing these cells to survive outside the bone marrow.

Spontaneous splenic rupture in a patient with acute promyelocytic leukaemia during induction chemotherapy

Adam Kuba, Tomas Szotkowski, Peter Rohon, Edgar Faber, Peter Turcsanyi, Jaromir Hubacek, Milena Holzerova, Vojtech Prasil, Marie Jarosova, Karel Indrak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):294-298 | 10.5507/bp.2013.086

Acute promyelocytic leukemia is the most curable subtype of adult acute leukemias. Fatal bleeding complications occur at diagnosis or during the induction part of treatment and remain a major cause of death. The authors report a case of a very rare spontaneous splenic rupture as a complication of initial antileukemic treatment in a patient with the highest risk of fatal bleeding, according to prognostic factors.

Serum cytokine and adhesion molecule profile differs in newly diagnosed acute myeloid and lymphoblastic leukemia

Jan M. Horacek, Tomas Kupsa, Martina Vasatova, Ladislav Jebavy, Pavel Zak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):299-301 | 10.5507/bp.2014.051

This paper compares serum levels of 17 cytokines and 5 adhesion molecules in patients with newly diagnosed AML and ALL using biochip array technology. The authors found significant increase in AML in serum IL-4, IL-2, IL-3, and significant decrease in serum VEGF and VCAM-1. The results show that the serum profile of cytokines and adhesion molecules differs in newly diagnosed AML and ALL patients.

The prevalence of non organ specific and thyroid autoimmunity in patients with polycystic ovary syndrome

Jana Petrikova, Ivica Lazurova, Ingrid Dravecka, Jana Vrbikova, Darina Kozakova, Jana Figurova, Zuzana Vaczy, Jan Rosocha

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):302-306 | 10.5507/bp.2014.062

It is the largest study on autoimmunity in PCOS patients with the widest spectrum of non organ specific autoantibodies to be examined to date. The authors found that the prevalence of non organ-specific autoantibodies in PCOS women was very low and similar or even lower than in healthy controls. They also found a slightly higher prevalence of thyroid autoimmuinity in PCOS women but this finding may have been related to the metabolic syndrome rather than to PCOS alone.

Correlation between magnetic resonance imaging and ultrasound measurements of eye muscle thickness in thyroid-associated orbitopathy

Marta Karhanova, Radim Kovar, Zdenek Frysak, Martin Sin, Jana Zapletalova, Jiri Rehak, Miroslav Herman

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):307-312 | 10.5507/bp.2014.001

This study on a large cohort of patients showed that the B-scan ultrasonographic measurement of horizontal eye muscles thickness in patients with thyroid-associated orbitopathy moderately correlates with values obtained using magnetic resonance imaging. The study confirmed that ultrasound is a very useful diagnostic tool for routine clinical practice in evaluation patients with thyroid-associated orbitopathy. The accuracy of ultrasonographic measurements significantly increases with the experience of the investigator.

Experience with the treatment of bisphosphonate-related osteonecrosis of the jaw

Zuzana Janovska, Radovan Mottl, Radovan Slezak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):313-317 | 10.5507/bp.2013.069

Bisphosphonate-related osteonecrosis of the jaw is a rare but severe side-effect of bisphosphonate use. This article summarizes the author's experience with the diagnosis and treatment of bisphosphonate-related osteonecrosis of the jaw during the years 2006-2012.

Risk of sympathectomy after anterior and lateral lumbar interbody fusion procedures

Lumir Hrabalek, Jan Sternbersky, Milan Adamus

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):318-326 | 10.5507/bp.2013.083

This is the first study, to the knowledge, of the authors to examine post-sympathectomy dysfunction objectively, using thermography after anterior or lateral interbody fusion of the lumbar spine (ALIF, XLIF) procedures, and the first study to evaluate clinically the severity of the post-sympathectomy syndrome!

Cutaneous silent periods in multiple system atrophy

Ivana Stetkarova, Markus Kofler, Veronika Majerova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):327-332 | 10.5507/bp.2013.081

The cutaneous silent period (CSP) is a spinal inhibitory reflex primarily mediated by A-delta fibers. In this study, the observed CSP prolongation concurred with previous findings in idiopathic Parkinson's disease (IPD). The lack of effect of levodopa on CSP abnormalities may aid in the differential diagnosis of multiple system atrophy versus IPD.

Oculocutaneous albinism in a patient with 17p13.2-pter duplication - a review on the molecular syndromology of 17p13 duplication

Marzena Kucharczyk, Aleksandra Jezela-Stanek, Dorota Gieruszczak-Bialek, Monika Kugaudo, Agata Cieslikowska, Magdalena Pelc, Malgorzata Krajewska-Walasek

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):333-337 | 10.5507/bp.2015.003

This paper describe a patient with a rare chromosomal aberration - a pure de novo subtelomeric 17p13.2-pter duplication. Despite known facial features, the proband presented with oculocutaneous albinism which has not been reported previously. The authors discuss in detail the genotype-phenotype correlations of the aberration as well as the possible molecular etiology of albinism apropos the mode of inheritance.

Renal denervation and glucose metabolism: another burst bubble?

Jan Vaclavik,

See Related Article, pp. 246-250.

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):338-340