Biomedical papers, 2015 (vol. 159), issue 1


News March 2015

Jitka Ulrichova, Vilim Simanek

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):vi

Review articles

The role of adhesion molecules in acute myeloid leukemia and (hemato)oncology: A systematic review

Tomas Kupsa, Jan M. Horacek, Ladislav Jebavy

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):001-011 | 10.5507/bp.2014.049

Adhesive interactions are vital for the persistence of both normal and malignant cells. Malignant cells trigger positive feedback loops causing adhesion molecule inflammatory overexpression. Disruption of adhesive interactions can overcome the high resistance to treatment and increase the selectivity of malignant cell targeting. Understanding adhesive interactions may underlie the basis of novel therapeutic approaches.

Chemical decellularization: a promising approach for preparation of extracellular matrix

Hana Hrebikova, Daniel Diaz, Jaroslav Mokry

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):012-017 | 10.5507/bp.2013.076

The extracellular matrix (ECM), which can be variously obtained, has several advantages such as providing the proper environment for cell attachment and proliferation. Decellularization techniques are very promising: they remove cell components, preserve tissue ECM and provides an optimal 3D environment for tissue engineering. This review covers chemical agents that are most widely used in decellularization methods.

Prostate Specific Antigen. Current clinical application and future prospects

Katerina Duskova, Stepan Vesely

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):018-026 | 10.5507/bp.2014.046

This review provides current insight into the clinical uses of Prostate Specific Antigen with focus on the controversies and future perspectives of this biomarker and including a summary of the latest research in this field.

Arteriovenous graft for hemodialysis, graft venous anastomosis closure - current state of knowledge. Minireview

Petr Bachleda, Petr Utikal, Martin Kocher, Marie Cerna, Jana Fialova, Lucie Kalinova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):027-030 | 10.5507/bp.2014.027

Venous anastomosis closure of arteriovenous graft (AVG) for hemodialysis leads to complete closure of AVG and loss of funtion. This article summarizes the evolution of the treatment of this complication. Currently preferred are endovascular and hybrid procedures.

Arthrocentesis of the temporomandibular joint: A review

Peter Tvrdy, Petr Heinz, Richard Pink

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):031-034 | 10.5507/bp.2013.026

Arthrocentesis of the temporomandibular joint is a minimally invasive method for treatment thatborders the conservative and the surgical. The main objectives of arthrocentesis are to wash out inflammatory mediators, release the disc, break adhesions, eliminate pain and improve joint mobility. It has become widespread and very popular in the treatment of internal disorders of the temporomandibular joint.

Therapeutic monitoring of psychoactive drugs - antidepressants: A review

Milan Grundmann, Ivana Kacirova, Romana Urinovska

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):035-043 | 10.5507/bp.2013.020

Therapeutic drug monitoring is a specific method of clinical pharmacology for monitoring drugs, using the measurement of drug serum concentrations followed by interpretation and good cooperation with clinician. It facilitates personalized medicine as dosing as rational therapy, minimising side effects, reducing negative consequences and cost of health care. Phenotyping and genotyping can improve monitoring of antidepressant drugs.

Alcohol withdrawal delirium - diagnosis, course and treatment

Barbora Mainerova, Jan Prasko, Klara Latalova, Karel Axmann, Monika Cerna, Rostislav Horacek, Romana Bradacova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):044-052 | 10.5507/bp.2013.089

This is a review on delirium tremens, the most severe complication of alcohol withdarawal and focused on pathogenesis, clinical symptoms, complications and proper, current management.

Original contributions

The anti-atherosclerotic effects of puerarin on induced-atherosclerosis in rabbits

Lidao Bao, Ying Zhang, Guomin Wei, Yi Wang, Ruilian Ma, Rui Cheng, Xianhua Ren, Agula B

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):053-059 | 10.5507/bp.2013.096

This research article explores the impact of puerarin in the pathogenesis of atherosclerosis in experimental atherosclerotic rabbits. The findings were that puerarin significantly attenuated the initiation and development of atherosclerotic lesions. Puerarin had anti-atherosclerotic effects through decreased expression of proliferating cell nuclear antigen (PCNA) and platelet-derived growth factor A (PDGF-A). In this way, puerarin attenuated the migration and proliferation of vascular smooth muscle cells (VMSCs).

Selected 68Ga-siderophores versus 68Ga-colloid and 68Ga-citrate: biodistribution and small animal imaging in mice

Milos Petrik, Adela Vlckova, Zbynek Novy, Lubor Urbanek, Hubertus Haas, Clemens Decristoforo

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):060-066 | 10.5507/bp.2014.052

This study elucidates the preclinical in vivo behaviour of selected 68Ga labelled siderophores versus radiochemical impurities, which can develop during 68Ga labelling and 68Ga-citrate as a representative of clinically used infection imaging agents. 68Ga-siderophores show great promise as highly selective, highly sensitive tracers for Aspergillus infection imaging. For translation into clinical practice more data on the pharmacokinetics and biodistribution of these promising new tracers are warranted.

Biological properties of a novel coladerm-beta glucan membrane.In vitro assessment using human fibroblasts

Sona Jantova, Dusan Bakos, Lucia Birosova, Patrik Matejov

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):067-076 | 10.5507/bp.2012.115

Samples of coladerm-beta glucan membrane (CBGM) were prepared using atelocollagen Hypro-Sorb®. Its biocompatibility, cytotoxicity, antimicrobial activity, genotoxicity and mutagenicity were then evaluated in vitro. The results show that the CBGM has good biocompatibility, antibacterial activity, no cytotoxicity/ genotoxicity/ mutagenicity and can be included as a potential scaffold for tissue engineering.

Lycopene improves the distorted ratio between AA/DHA in the seminal plasma of infertile males and increases the likelihood of successful pregnancy

Radka Filipcikova, Ivana Oborna, Jana Brezinova, Jiri Novotny, Gabriella Wojewodka, Juan Bautista De Sanctis, Lenka Radova, Marian Hajduch, Danuta Radzioch

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):077-082 | 10.5507/bp.2013.007

The potent antioxidant - carotenoid lycopene had positive effect at the level of oxidative stress in seminal plasma of males incases of infertile couples. There was an improved ratio of two polyunsaturated fatty acids - arachidonic acid and docosahexaenoic acid. Although there were no significant changes in semen parameters, there wasincreased chance for achieving spontaneous conception as well as likelihood of pregnancy after IVF treatment.

Breast cancer detection using combined curvelet based enhancement and a novel segmentation methods

Balasubramaniam Senthilkumar, Govindaswamy Umamaheswari

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):083-086 | 10.5507/bp.2013.097

Curvelet based noise removal and a new segmentation method not tried before were tested for the detection of breast cancer. The results were better than existing methods.

Detection of nano- and micro-sized particles in routine biopsy material - pilot study

Jana Dvorackova, Hana Bielnikova, Jana Kukutschova, Pavlina Peikertova, Peter Filip, Karol Zelenik, Pavel Kominek, Magdalena Uvirova, Jana Pradna, Zuzana Cermakova, Igor Dvoracek

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):087-092 | 10.5507/bp.2012.104

The presence of metallic particles in pathologically altered tissues may signal an alternative causation of some diseases. The ethiopathogenic explanation of the diseases associated with the presence of nano-sized particles in the organism is emerging as a new field of pathology, nanopathology.

Evaluation of biochemical markers and bone mineral density in patients with chronic kidney disease stage 5D at the start of hemodialysis treatment

Ivo Valkovsky, Renata Olsanska, Josef Tvrdik, Arnost Martinek, Zdenek Svagera, Marketa Pernicova, Jarmila Dedochova, Zuzana Cermakova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):093-099 | 10.5507/bp.2013.087

Patients with chronic kidney disease have significant disorders in bone and mineral metabolism. This study evaluated the bone mineral density (BMD) of patients at the start of hemodialysis treatment and the relationship between BMD and possible risk factors or biochemical markers.

Incidence of fecal Enterobacteriaceae producing broad-spectrum beta-lactamases in patients with hematological malignancies

Milan Kolar, Miroslava Htoutou Sedlakova, Vendula Pudova, Magdalena Roderova, Jiri Novosad, Marketa Senkyrikova, Romana Szotkowska, Karel Indrak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):100-103 | 10.5507/bp.2014.042

The ESBL- and AmpC-positive Enterobacteriaceae in the normal gastrointestinal tract (GIT) microflora are potential etiological agents whose high resistance to antibiotic treatment can lead to a failure of initial antibiotic therapy. Some authors do not consider monitoring of these strains as useful. However, our study identified two patients diagnosed with urinary tract and bloodstream infections caused by ESBL- or AmpC-positive Enterobacteriaceae from the GIT microflora. This is evidence that these strains can act as agents causing bacterial complications and in patients with hematological malignancies, it is advisable to regularly monitor the prevalence of both pathogenic multiresistant strains and potentially pathogenic strains found in GIT.

Oligoclonal free light chains in cerebrospinal fluid as markers of intrathecal inflammation. Comparison with oligoclonal IgG

David Zeman, Pavel Hradilek, Pavlina Kusnierova, Radim Piza, Katarina Reguliova, Ivana Woznicova, Olga Zapletalova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):104-113 | 10.5507/bp.2014.058

The sensitivity and specificity of CSF-restricted oligoclonal IgG and oligoclonal free light chains are compared for the ability to diagnose multiple sclerosis and other inflammatory neurological diseases. Optimal cut-off values for the number of CSF-restricted oligoclonal IgG and free light chain bands were analyzed; for oligoclonal IgG and free light chains, these cut-offs were found to be higher (≥4 IgG bands and ≥6 free κ bands) than the cut-off ≥2 bands usually reported so far. The general conclusion is that reporting borderline values as positive, compromises the specificity of these tests. The oligoclonal free light chain test may be valuable especially when the oligoclonal IgG test is negative or borderline.

Variability of post-exercise pulmonary capillary wedge pressure recovery. Implications for noninvasive echocardiographic diagnostics

Jaroslav Meluzin, Petr Hude, Jan Krejci, Lenka Spinarova, Pavel Leinveber, Radka Stepanova, Petr Nemec

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):114-119 | 10.5507/bp.2013.016

Seventy-one patients at risk of heart failure with normal left ventricular ejection fraction underwent simultaneous exercise echocardiography and right heart catheterization. Fifty-one patients had exercise-induced pulmonary capillary wedge pressure (PCWP) elevation ≥ 8 mmHg and reached peak exercise PCWP ≥ 20 mmHg. Post-exercise recovery of PCWP at 1, 2, 3, 4, and ≥ 5 min was achieved in 7 (13.7%), 11 (21.6%), 10 (19.6%), 6 (11.8%), and 17 (33.3%) patients, resp. In summary, the course of post-exercise PCWP recovery is highly variable, and a significant proportion of patients have only a brief period (≤ 2 min) of exercise-induced PCWP elevation. This fact should be taken into account when assessing exercise-induced PCWP noninvasively.

Treatment of central serous chorioretinopathy with beta-blocker metipranolol

Oldrich Chrapek, Barbora Jirkova, Vít Kandrnal, Jiri Rehak, Martin Sin

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):120-123 | 10.5507/bp.2013.015

In the treatment of central serous chorioretinopathy, we used the beta-blocker metipranolol. Metipranolol use is associated with possible adverse effects such as: indigestion, fatigue, weakness, malaise, headache, dizziness, insomnia, depression, itching, skin rash, visual disturbances, shortness of breath, slow heart rate and other heart rhythm disorders, low blood pressure , dryness of the conjunctiva. This study showed no benefit of metipranolol for the treatment of central serous chorioretinopathy. We believe that metipranolol is not appropriate for the treatment of patients with central serous chorioretinopathy, exposing them to the risk of the adverse events associated withmetipranolol.

Self care in patients with chronic heart failure. Pilot study - self care includes problems

Renata Halmo, Jan Galuszka, Katerina Langova, Dana Galuszkova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):124-130 | 10.5507/bp.2013.044

Patients with chronic heart failure (HF) need self-care training to integrate their health problem into everday life. The article maps the level of patients' problems and level of self-care actions performed by patients with HF.

Impact of retransfusion of blood processed in cell-saver on coagulation versus cardiopulmonary bypass: A prospective observational study using thromboelastography

Tomas Vymazal, Martin Filaun, Michal Horacek

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):131-134 | 10.5507/bp.2013.012

This paper compares the impact of retransfusing patient's blood processed in cell-saver and cardiopulmonary bypass on hemocoagulation. The authors analyzed thromboelastographic (TEG) samples from 170 patients who had undergone cardiac surgery with and without cardiopulmonary bypass. The results show similar effects to hemocoagulation in cardiac patients.

Analysis of thymidine kinase serum levels by novel method DiviTum™ in multiple myeloma and monoclonal gammopathy of undetermined significance - comparison with imaging methods 99mTc-MIBI scintigraphy and 18F-FDG PET/CT

Jaroslav Bacovsky, Miroslav Myslivecek, Jiri Minarik, Vlastimil Scudla, Tomas Pika, Jana Zapletalova, Pavla Petrova, Margita Bartkova, Tomas Adam, Simon J Gronowitz

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):135-138 | 10.5507/bp.2014.008

Proliferation is an independent prognostic factor in multiple myeloma. Increased activity of thymidine kinase in serum is associated with higher proliferation rate in multiple myeloma. Imaging methods such as 99mTc-MIBI scintigraphy and 18F-FDG PET/CT imagine active myeloma lesions and a positive result is a negative prognostic factor. Increased levels of serum thymidine kinase were observed in MIBI or PET/CT positive patients.Combination of increased levels of serum thymidine kinase and positive MIBI or PET/CT results are useful prognostic factors.

Hepatic arterial infusion in hepatocellular carcinoma: a single center experience

Bohuslav Melichar, Josef Dvorak, Alexander Ferko, Katerina Kamaradova, Antonin Krajina

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):139-144 | 10.5507/bp.2014.054

This retrospective analysis summarizes single-center experience with hepatic arterial infusion (HAI) in hepatocellular carcinoma (HCC). Published experience with HAI in HCC in Western countries is sparse, and the present data demonstrate limited efficacy of this therapeutic approach.

Association between rs267196 and rs267201 of BMP6 gene and osteonecrosis among Sickle Cell Aneamia patients

Leila Chaouch, Miniar Kalai, Manel Ben Jbara, Arij Ben Chaabene, Imen Darragi, Dorra Chaouachi, Fethi Mallouli, Raouf Hafsia, Abderraouf Ghanem, Salem Abbes

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):145-149 | 10.5507/bp.2013.080

To the best of our knowledge, this is the first report in the study of the impact of modifier gene in the occurrence of osteonecrosis in sickle cell anemia in Tunisian patients. Significant associations with osteonecrosis were found with 2 SNPs namely: rs267196 and rs267201 of BMP6 gene.

Resection versus biopsy of glioblastomas in eloquent brain areas

Lumir Hrabalek, Ondrej Kalita, Miroslav Vaverka, Miloslava Zlevorova, Jiri Ehrmann Jr, Eva Cechakova, Milan Adamus, Vlastimil Novak, Katerina Langova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):150-155 | 10.5507/bp.2013.052

This is the first prospective study that compares the results (neurological deterioration, overall survival time) of resection and biopsy of glioblastoma (GBM) in eloquent brain areas (EBA). For patients in good clinical condition and for tumors in or near EBA, resection of the GBM (as radical as possible) is recommended.

Knowledge, attitudes and decision-making in Czech women with atypical results of prenatal screening tests for the most common chromosomal and morphological congenital defects in the fetus: Selected questionnaire results

Vladana Skutilova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):156-162 | 10.5507/bp.2013.046

The paper describes the variables that can complicate compliance and decision-making in pregnant women coming for genetic counselling based on atypical screening of one of the screening tests, or an issue in personal or family history. This is the first study done in in the Czech environment. Among other things, lack of awareness and widespread confusion about screening and invasive tests were discovered, creating the distress that pregnant women here often unnecessarily experience.

Case reports

Secondary hypertension and hirsutism as a clinical manifestation of tumor duplicity

Zdenek Frysak, David Karasek, Igor Hartmann, Ladislava Kucerova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(1):163-165 | 10.5507/bp.2013.056

Primary aldosteronism is a relatively common cause of secondary hypertension. Hyperandrogenic conditions tend to have their origin in the adrenal cortex. The authors report a very rare combination of adrenocortical adenoma as a source of overproduction of aldosterone and benign tumor of the ovary, as the source of testosterone production. The patient was completely healed by one laparoscopic procedure.