Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):327-338 | 10.5507/bp.2014.025
The aim of the review was to assess current uses of heart rate variability (HRV) in cardiac autonomic neuropathy (CAN) diagnosis with emphasis on proven methodological approaches and current views on the physiological interpretation and clinical uses of HRV methods. In clarifying controversial viewpoints on HRV, the paper supports its use in the routine diagnosis of neuropathic diseases.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):339-345 | 10.5507/bp.2013.084
This is an overview of new molecular techniques (array-based analysis, Multiplex Ligation-depend Probe Amplification (MLPA) and next generation sequencing), their clinical applications in patients with MDS (myelodysplastic syndromes). Somatic mutations may influence the clinical phenotype but are not included in current prognostic scoring systems. Advantages, limitations and future perspectives of these new methods are discussed.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):346-355 | 10.5507/bp.2014.034
This article covers key information on the use of amino acids including how much in the context of nutritional support for ICU patients. It is a summary of the vital role of nutritional support. The research shows that inappropriate composition of amino acids and/or insufficient amounts contributes to malnutrition which may cause increased risk of morbidity and mortality in ICU settings.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):356-360 | 10.5507/bp.2013.051
This paper reviews the characteristics of MSCs derived from fetal appendages as a possible good alternative to bone marrow sources. MSCs of different origin are described as having comparable characteristics in terms of molecular and cellular biology. As the percentage of MSCs in bone marrow is very low and retrieval techniques are risky, it is important to find alternative sources of MSCs, such as extra-embryonic tissues which represent a promising tool in regenerative medicine
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):361-364 | 10.5507/bp.2012.098
This is a review of the latest information on the role of neuroactive steroids in the development of mental disorders, especially in post-partum "blues" and post-partum depression.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):365-372 | 10.5507/bp.2012.112
This review covers the current data on a group of products with intrinsic fluorescent properties, called lipofuscin-like pigments. Lipofuscin-like pigments are formed by reaction of lipid peroxidation intermediates and biological amine-containing compounds. Since free radical-mediated lipid peroxidation is implicated in the pathophysiology of a number of diseases, specific lipofuscin-like pigments are potential biomarkers for the pre-clinical stages and/or as risk factors for these diseases.disease. Chemical characterization using advanced analytical tools holds promise for the future use of lipofuscin-like pigments in biomedicine.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):373-377 | 10.5507/bp.2013.078
Dental pulp stem cells are a promising source of cells for numerous regenerative medicine applications both in dentistry and in neurological regenerative medicine. Comparison of the expression of pluripotent stem cell genes at the protein level shows that dental pulp stem cells differ from bone marrow, adipose tissue and umbilical cord mesenchymal stem cells significantly. Differences in the gene expression of dental pulp mesenchymal stem cells fully reflect their developmental origin in the neural crest.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):378-383 | 10.5507/bp.2012.090
Pythium insidiosum, a fungus-like organism, can cause an endemic, life-threatening disease restricted mostly to Thailand. Currently, there are neither effective antimicrobials nor vaccines against this infection available. In this study, environmental bacterial strains were screened for their ability to inhibit the growth of this oomycete. Anti-P. insidiosum activity was found in 16 isolates. In one strain, Pseudomonas stutzeri ST1302, a subfraction labelled PYK7, assigned to the diketopiperazines, exhibited exceptionally strong activity. Search for genes encoding compounds with known anti-P. insidiosum activity was also performed within the genome of this P. stutzeri strain; one gene region, corresponded to pyrrolnitrin, was found. To the best of the knowledge of the authors, this is the first report on the inhibitory effect of P. stutzeri against P. insidiosum
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):384-390 | 10.5507/bp.2012.105
The efficacy of spironolactone in different age and sex subgroups, the authors analysed the ASPIRANT trial. They found that spironolactone in a daily dose of 25 mg lead to a larger reduction in systolic BP in older patients >62 years compared to younger patients, and was equally effective in men and women.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):391-396 | 10.5507/bp.2013.003
Early recanalisation of the occluded cerebral artery in acute ischemic stroke patients.plays a major role in improvement Treatment with intravenous thrombolysis is associated with high probability of poor outcome with low recanalization rates. Intraarterial thrombolysis may be more effective but treatment can be delayed by catheterization. The combination of IV and IA thrombolysis allows faster start of thrombolysis using the IV route and higher recanalization rate using the intraarterial approach. This article reports the safety and efficacy of combined IV + IA thrombolysis compared to IV thrombolysis alone in acute ischemic stroke patients with occluded middle cerebral artery.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):397-403 | 10.5507/bp.2013.050
Amniotic fluid embolism (AFE) is a rare, but very severe obstetric complication. The clinical diagnosis is one of exclusion. This paper presents six fatal cases of AFE in Slovakia from 2005-2010 and compares the incidence, possible risk factors, neonatal outcomes and the clinical course of cases in Slovakia with fatal cases of AFE in the UK during the same period.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):404-411 | 10.5507/bp.2013.054
Accelerated atherosclerosis in systemic lupus erythematosus (SLE) is a serious cause of morbidity and mortality. This paper provides a comprehensive overview of the possible etiology of atherosclerosis in patients with SLE. Traditional risks factors (lifestyle, lipid spectrum, hypertension, diabetes), immunology profile, markers of endothelial dysfunction, intima media thickness, medication, disease activity and damage are covered. Subgroups with and without lupus nephritis are compared with a healthy population.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):412-421 | 10.5507/bp.2013.053
The MEDINA (MEtabolic parameters, DIabetes mellitus and NephropAthy) study compares angiotensin converting enzyme inhibitors (ACE-I) with angiotensin receptor blockers(ARB) in monotherapy and combined therapy with diuretic and/or a calcium-channel blocker (CCB) in 439 hypertensive patients with metabolic syndrome and/or diabetes mellitus. The blood pressure decrease difference between ACE-I and ARB was insignificant. Adding either hydrochlorothiazide or amlodipin was equally effective. There were no significant differences in metabolic parameters. The MEDINA trial has confirmed the equivalence of ACE-I and ARB in hypertension treatment and a combination with diuretics or CCB has an additive effect. Adding statins can further reduce the cardiovascular risk.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):422-427 | 10.5507/bp.2012.059
This in vitro study shows the high inter-individual variability of leukemia cell response to prednisolone and dexamethasone. Hence, they compared the in vitro activity of prednisolone and dexamethasone in tandem. The combination of glucocorticoids, particularly the low and safe dosage of dexamethasone exhibited much greater in vitro potency than prednisolone alone (P<0.0006), which suggests the potential benefit of glucocorticoid combination in the treatment of childhood ALL.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):428-432 | 10.5507/bp.2013.022
This study reports no impact of primary chemotherapy delay resulting in reduced relative dose intensity of administered cytostatics on the outcome of patients with Hodgkin's lymphoma if the dose density of these drugs had remained the same according to appropriate protocol. Thus, time intensification of some therapeutic protocols appears to have no significant impact on the prognosis of patients with Hodgkin's lymphoma..
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):433-437 | 10.5507/bp.2012.091
The authors discuss the possible role of the tumor-stroma microenvironment in the local recurrence of rectal carcinoma. On analysis of two case reports, they demonstrate that changes in microenvironment around the anastomoses, mainly in association with the surgical procedure may create favourable conditions for recruitment, settling and subsequent proliferation of circulating tumor cells from minimal residual disease.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):438-446 | 10.5507/bp.2012.081
The Czech Republic was found to have the fifth highest recorded incidence of malignant cutaneous melanoma in Europe in 2008 in men and ninth in women. In this article, the causes of the rising trends and explanation of differences in development of cutaneous melanoma between men and women, according to age and disease stage are reported.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):447-450 | 10.5507/bp.2012.082
This article provides preliminary results of resection procedures for locally advanced low rectal cancer combined with intraoperative radiotherapy (IORT). The authors describe the technical feasibility of this method and stress its importance in the multimodal treatment of colorectal carcinoma. Although IORT is associated with higher risk of peri- and postoperative complications, it improves local control in patients with advanced tumours of the lower rectum.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):451-454 | 10.5507/bp.2012.077
Radiofrequency ablation is a method of choice in the treatment of cancer of the lung. The advantage of this treatment method is the simplicity and posibility of combination with other procedures.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):455-460 | 10.5507/bp.2013.045
Gastric neuroendocrine neoplasms are to heterogeneous group of gastro-entero-pancreatic tumors characterized by lower malignant potential, rising incidence, and idiosyncratic biological tumor behavior. There are three types of gastric neuroendocrine neoplasms that distinctly differ in biological and clinicopathological characteristics, as well as in different management. The article provides an overview of neuroendocrine tumor classification, their clinical characteristics, histological findings, laboratory measurement, prognosis, and therapeutic strategy. The authors present the clinical features and treatment of twenty-five patients with gastric neuroendocrine neoplasms that were diagnosed and treated by them.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):461-464 | 10.5507/bp.2013.032
The authors summarize the facts and their clinical experience with omitting adjuvant radiotherapy in the early stages of breast cancer in elderly patients. Adjuvant radiotherapy is standard treatment after breast conserving surgery but in elderly patients the benefit of radiotherapy is unclear. The experience of the authors is that adjuvant radiotherapy may be safely omitted in older patients with low risk breast cancer.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):465-469 | 10.5507/bp.2012.074
This paper assesses the use of color Doppler ultrasound in the pre-histological determination of the biological features of salivary gland tumors. Color Doppler parameters (peak systolic velocity, resistive index, pulsatility index) were used to differentiate between patients withbenign, low-grade and high-grade salivary gland tumors. The ultrasonographic results are compared with final histological results.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):470-473 | 10.5507/bp.2013.011
X-linked agammaglobulinemia (XLA) is a rare inherited primary immunodeficiency. The authors report the clinical case of a boy with XLA with delayed diagnosis despite typical clinical features of severe antibody immunodeficiency. The disease was caused by a new, previously undescribed mutation in the BTK gene. This case report summarizes the crucial and typical clinical symptoms of XLA. Recognising the warning signs of primary immunodeficiencies could substantially help to improve the prognosis of these patients.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):474-478 | 10.5507/bp.2013.040
Complete surgical excision is the only option for prolonging survival in the case of this rare entity. A 27-month disease-free survival after surgical resection alone in the case presented, underscores the importance of resection even in a patient with a giant tumour. Potential space constraints in large tumours in this localisation may require en bloc resection with neighbouring organs.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):479-485 | 10.5507/bp.2013.058
Hereditary spherocytosis refers to a group of heterogeneous inherited anemias. The two most often affected organs are the spleen and gallbladder. Splenectomy is the only effective therapy for this disorder and often it is performed in combination with cholecystectomy. The authors have experienced positive results from performing laparoscopic splenectomy and cholecystectomy at one time for treating hereditary spherocytosis that was combined with cholelithiasis. Their experience shows that single incision laparoscopic surgery splenectomy and cholecystectomy is feasible even in young children and despite the small number of cases in the world, they consider the combined laparoscopic approach, safe and effective for the treatment of hereditary spherocytosis
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(3):486-487 | 10.5507/bp.2014.047