Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):161-169 | 10.5507/bp.2013.085
This is a review of publications on the mechanisms of the anticancer effects of histone deacetylase (HDAC) inhibitors and the results of the most important clinical studies. HDACs are involved in different cellular pathways and functions. Their inhibitors are promising anti-cancer drugs to be used either alone or in combination with other anti-cancer drugs. Two HDAC inhibitors (vorinostat, romidepsin) have been approved for cutaneous T-cell lymphoma and romidepsin for peripheral T-cell lymphoma. Many others are in clinical trials for the treatment of malignancies. Even though the many biological and clinical effects of HDAC inhibitors have been described, explanations and their role in complex anticancer therapy remain unclear.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):170-174 | 10.5507/bp.2012.048
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):175-180 | 10.5507/bp.2014.021
This review summarizes the latest information on the role of HIF-2α in the development of neuroendocrine tumors, especially pheochromocytoma and paraganglioma.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):181-188 | 10.5507/bp.2014.016
In recent years, there has been an increasing interest in the clinical features and pathophysiological mechanisms of xanthomas. Most types of xanthomas develop as cholesterol-rich lesions within the skin, tendons, fasciae or periosteum. They are characterized by local accumulation of foam cells and resemble early stages of atherosclerotic plaques. The presence of xanthomas can signalise serious dyslipidemias, both common and rare, connected with an increased risk for cardiovascular, metabolic and tumorous diseases. This article provides an overview of key research findings on xanthomas, mainly from the clinical point of view.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):189-193 | 10.5507/bp.2013.019
Minimal residual disease (MRD) in lung cancer is defined as isolated or circulating tumor cells in a patient who has hada curative primary lung tumor removal and in the same period, there are no clinical signs of cancer. Progress in the diagnostics of lung cancer is greatly limited by the lack of diagnostic markers. MRD detection is important in the determination of disease progression and for the prognosis of lung cancer. In 2001, a promising new lung-specific tumor marker LunX was identified, but we still need to verify it using the marker in the clinical treatmentof patients.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):194-200 | 10.5507/bp.2014.018
Our experiments revealed that cisplatin can inhibit Na+/K+-ATPase (NKA), suggesting that NKA/cisplatin interaction is an important factor in cisplatin adverse effects. Using chronopotentiometry and mass spectrometry, we found that cisplatin is bound to the large cytoplasmic segment connecting transmembrane helices TM4 and TM5 (C45).
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):201-207 | 10.5507/bp.2012.057
This article describes the use of photodynamic therapy applied to cell lines and bacterial strains. Due to continuous increase in bacterial resistance to antibiotics, the target of the experimental work was to find other options for microbial disease treatment.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):208-211 | 10.5507/bp.2012.083
This study provides new information on the antimicrobial effects of selected natural substances against clinical isolates of Mycoplasma hominis about which very little is known. To date, only the inhibitory effects of three substances - oleuropein, hydroxythyrosol and tea tree oil against Mycoplasma hominis strains have been published. The results of this study indicate that for example cinnamon bark oil, carvacrol and eugenol might be considered as promising antimicrobial agents for the therapeutic treatment of mycoplasmal infection.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):212-220 | 10.5507/bp.2013.024
The role of zinc-finger transcription factor Crz1 in hypoxic adaptation and cell wall integrity is described in the human pathogenic strictly aerobic basidiomycetous yeast Cryptococcus neoformans. Whereas the role of various Crz1 homologues in cell wall integrity is well- known in other fungal species, this is the first time that its role in hypoxic adaptation has been documented, indicating a tight link between cell wall integrity, hypoxia and cell cycle slowdown/arrest in G2.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):221-226 | 10.5507/bp.2012.076
This article summarises pilot animal experiments with our novel micro-catheter technology for endovascular magnetically guided interventions in the central nervous system. By using the external magnetic field generated by the NIOBE Stereotaxis technology, the micro-catheter can be safely and easily navigated into targeted areas in the brain vasculature, including areas with extremely high tortuosity of arterial or venous systems. Further, the micro-catheter technology is capable of endovascular sensing of EEG signal in the targeted area. To the best of our knowledge, this is the first micro-catheter technology developed so far for magnetically guided interventions in the central nervous system, opening new opportunities for interventional treatment of stroke, epilepsy and several others CNS disorders.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):227-232 | 10.5507/bp.2012.073
Use of remifentanil in labour is not very widespread even though it is a safe and effective alternative to epidural analgesia. Remifentanil administration in Parturient Controlled Analgesia (PCA) mode is relatively well-tolerated and increases the satisfaction of the parturient with pain management. The aim of the presented study was to compare the analgesic efficacy of PCA intravenous remifentanil administration with epidural analgesia during the first stage of labour with regard to maternal and early neonatal side-effects. We also aimed to confirm a clinically very simple and unique method (rigid dosage regimen of remifentanil) of PCA remifentanil administration. We concluded that remifentanil is an effective analgesia with high parturient satisfaction scores and clinically good neonatal outcomes.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):233-237 | 10.5507/bp.2012.064
The study aimed to assess the safety and efficacy of bilateral sonothrombolysis of acute occlusion of the middle cerebral artery using 2 diagnostic transcranial probes. Twelve acute stroke patients with middle cerebral artery occlusion contraindicated for thrombolysis underwent bilateral sonothrombolysis and 37 patients underwent standard sonothrombolysis. There were no differences in complete recanalization of the artery after 60 minutes (30.0% vs. 32.4% of patients), independence after 90 days (58.3% vs. 59.5% of patients) and symptomatic intracerebral hemorrhage (0% vs. 2.7% of patients). Sonothrombolysis using 2 probes seems to be safe but not more effective than standard sonothrombolysis.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):238-241 | 10.5507/bp.2012.070
The carriers of thrombophylic mutation, if pregnant, are thought to be at higher risk of developing serious late pregnancy complications such as preeclampsia, HELLP syndrome, intrauterine growth restriction and even intrauterine death of the fetus. The heterozygous configuration we found in the great majority of this pregnant patient group. The aim of the study was to show whether there was any relationship between the carriage of thrombophylic mutation in heterozygous configuration and the higher incidence of any of the monitored serious late pregnancy complications. The results might help to decide whether prophylaxis with LMWH should be used to improve the pregnancy outcome.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):242-258 | 10.5507/bp.2013.070
The aim of the study was to evaluate, whether the combination of skin graft (DESG), autologous thrombin and autologous platelet concentrate (APC) in deep burn trauma treatment leads to the development of stable, non-hypertrophic scarring. The results showed that patients treated with DESG/APC had superior healing without any evidence of scar hypertrophy or contractures. One of the most important findings of this study was the cost effectiveness of the therapy: the treatment was associated with earlier discharge from hospital and significant cost savings.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):259-264 | 10.5507/bp.2013.059
The technique of iterative CT image reconstruction is capable of a substantial reduction in both breast radiation dose and effective dose in pulmonary CT angiography. When compared to the standard filtered back projection, the lower radiation dose achieved with iterative image reconstruction was also associated with a significantly better objective and subjective image quality.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):265-272 | 10.5507/bp.2013.071
Iterative reconstruction of cerebral CT examinations enables reduction of both effective and organ eye lens dose by one third compared to the standard filtered back projection, without signficant loss of image quality.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):273-276 | 10.5507/bp.2012.024
Globally more than 1,000,000 patients undergoing Total Hip Arthroplasty (THA) each year are at risk for Prosthetic Joint Infection (PJI). Identification of risk factors associated with PJI could facilitate preventive measures. Osteoprotegerin (OPG; official gene symbol: TNFRSF11B) is considered a negative regulator of bone resorption. A genetic variation in the OPG gene is hypthesised to contribute to the risk of PJI. However, this study did not show any association between the OPG -163 T/C SNP and PJI in a Czech population, casting doubt on the use of OPG -163 T/C determination as a risk factor for PJI.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):277-281 | 10.5507/bp.2012.060
Nondisplaced and minimaly displaced fractures of the scaphoid waist can be surgically treated using volar or palmar miniinvasive approaches. This prospective randomised study compared both methods for union rate, complications, functional results and DASH score in 76 patients. Although we found some differences during the follow-up examinations at eight and twelve weeks, no differences in results or complications were statistically signifiant up to one year follow up.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):282-287 | 10.5507/bp.2013.002
By combining both the enumeration of AgNORs and their size, good distinction can be made between normal, dysplastic and infiltrating squamous cell carcinomas. This could help in the early diagnosis and prognosis of dysplastic mucosal lesions and their malignant transformation.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):288-294 | 10.5507/bp.2013.062
This study aimed at identifying risk factors and early stages of selected diseases/comorbidities in patients with psoriasis. The study was focused on Czech psoriatic patients because international research results are highly variable and the conclusions are ambiguous. No similar study has been performed to date in Czech psoriatic patients. The results show significant differences between cases and controls in several parameters. For this reason, regular screening of patients with the chronic stationary form of psoriasis should become a standard for dermatologists in cooperation with general practitioners.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):295-302 | 10.5507/bp.2013.095
This is the first study to describe the high prevalence of isolated exercise-only induced heart failure with normal ejection fraction (HFNEF) reaching 58% in a cohort of 81 post-heart transplant patients with normal left ventricular ejection fraction. This finding explains their low exercise tolerance due to exertional dyspnea and/or fatigue. Of the noninvasive parameters obtained at rest, multivariate regression analysis identified left ventricular mass index adjusted for allograft age to be an independent predictor of exercise-induced HFNEF.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):303-308 | 10.5507/bp.2013.010
Diabetes self-management is the cornerstone of effective diabetes care. A better understanding of variables pertinent to self-care behaviour in people with diabetes may provide valuable insight for diabetes educators regarding the multifaceted impact of this complex disease. The study investigated differences in self-care behaviours, treatment satisfaction, and quality of life in people on insulin treatment according to the structured educational programme in spe.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):309-312 | 10.5507/bp.2012.071
Donor granulocytes transfusions are used as a therapeutical modality in immunocompromised patients unresponsive to antibiotic/antifungal therapy. At the Blood Centre, Faculty Hospital Ostrava, granulocytes are obtained from unrelated donors after their mobilization with methyl prednisolone. Currently used protocols show that the collection process is standardized and independent of haematocrit levels in granulocyte unit (hct/1TU GA).
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):313-314 | 10.5507/bp.2012.058
In medical practice, it is of the utmost importance to follow strictly, definitions, formulas, and units defining exact parameters, pathological situations, and diseases. The purpose of this short communication was to show some inconsistencies and errors even in very reputable cardiological journals and to issue a caveat. We demonstrate examples of confusing definitions and formulas concerning two frequently used echocardiographic parameters, left ventricular mass and relative wall thickness, which may lead to different results and conclusions. The paper concludes that it is desirable to follow exactly the definitions of complex parameters and to unify ways of presenting those with several definitions.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):315-320 | 10.5507/bp.2012.067
This work describes the effect of two positive mutagens in the development of ciprofloxacin resistance in Salmonella Typhimurium. The mechanism of resistence was also studied in isolated resistant strains. Low concentrations of 2-nitrofluorene (2NF) and 3-(5-nitro-2-furyl) acrylic acid (5NFAA) significantly increased mutation frequency, leading to ciprloxacin resistance. 2NF was the best inducer of mutations in the gyrA gene and in regulating genes affecting synthesis inhibition of outer membrane porins. 5NFAA effected overproduction of efflux pumps.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(2):321-325 | 10.5507/bp.2013.077
Trisomy 21 - Down syndrome (DS) - is a well- known chromosomal aberration with a typical clinical picture. The partial trisomies/tetrasomies of chromosome 21 are rare findings. In our original article we report genotype-phenotype correlation in two cases of partial trisomy and tetrasomy of chromosome 21. Our observation can help to narrow the region responsible for DS and to map the loci accountable for minor features of DS. The description of the infrequent origin of these aberrations is included.