Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2025, 169(2):123-131 | DOI: 10.5507/bp.2024.002

Oxidative stress, microparticles, and E-selectin do not depend on HIV suppression

Katerina Havlickova1, Svatava Snopkova1, Miroslav Pohanka2, Radek Svacinka1, David Vydrar1, Petr Husa Jr.1, Jirina Zavrelova3, Filip Zlamal4, Lenka Fabianova5, Miroslav Penka3, Petr Husa1
1 Department of Infectious Diseases, Faculty Hospital Brno and Faculty of Medicine, Masaryk University, Brno, Czech Republic
2 Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
3 Department of Hematology, Faculty Hospital Brno and Faculty of Medicine, Masaryk University, Brno, Czech Republic
4 Research Centre for Toxic Compounds in the Environment, Masaryk University, Brno, Czech Republic
5 Department of Infectious Diseases, Masaryk Hospital in Usti nad Labem, Czech Republic 

Background: Oxidative stress and inflammation are considered predictors of diseases associated with aging. Markers of oxidative stress, inflammation, and endothelial activation were investigated in people with HIV on antiretroviral treatment to determine whether they had an immunosenescent phenotype that might predispose to the development of premature age-related diseases.

Patients and Methods: This study was conducted on 213 subjects with HIV. The control groups consisted of healthy HIV-negative adults. The level of oxidative stress was measured by assessing the production of malondialdehyde levels, which were detected by thiobarbituric acid reactive substance (TBARS) assay. The level of microparticles indicated the presence of inflammation and endothelial activation was measured by E-selectin levels. Significant differences were determined by appropriate statistical tests, depending on the distribution of variables. Relationships between continuous variables were quantified using Spearman's rank correlation coefficient.

Results: TBARS, and microparticle and E-selectin levels were significantly higher in untreated and treated subjects with HIV compared with HIV-negative controls (P<0.001). The levels of the investigated markers were not significantly different between untreated and treated patients and no significant correlation of these markers was found with CD4+ count, CD4+/CD8+ ratio, and the number of HIV-1 RNA copies.

Conclusions: Elevated markers of oxidative stress, inflammatory and endothelial activation were independent of the virologic and immunologic status of people with HIV. These results support the hypothesis that residual viremia in cellular reservoirs of various tissues is a key factor related to the premature aging of the immune system and predisposition to the premature development of diseases associated with aging.

Keywords: oxidative stress, microparticles, E-selectin, HIV suppression

Received: December 4, 2023; Revised: January 21, 2024; Accepted: January 26, 2024; Prepublished online: February 23, 2024; Published: June 1, 2025  Show citation

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Havlickova, K., Snopkova, S., Pohanka, M., Svacinka, R., Vydrar, D., Husa, P., ... Husa, P. (2025). Oxidative stress, microparticles, and E-selectin do not depend on HIV suppression. Biomedical papers, 169(2), 123-131. doi: 10.5507/bp.2024.002
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