Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(4):335-340 | DOI: 10.5507/bp.2018.069

Various surgical techniques for the repair of injured vas deferens in rat experiments

Radek Stichhauera,b, Jaroslav Koudelkaa,b, Ales Ryskac, Helena Zivnad, Milan Kaskab
a Department of Paediatric Surgery, University Hospital Hradec Kralove, Czech Republic
b Academic Department of Surgery, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic
c Fingerland Department of Pathology, Faculty of Medicine in Hradec Kralove, Charles University and University Hospital Hradec Kralove, Czech Republic
d Radioisotope Laboratories and Vivarium, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic

Objective: The aim of this study was to evaluate whether the different vasovasostomy techniques can be performed using only the operating loupe in a rat model. The secondary aims were to evaluate the patency rate and inflammation of the vas deferens (VD) after contusion and the different vasovasostomy repair techniques.

Methods: A total of 40 male rats were divided into 4 groups based on the type of surgery: 1. contusion of the VD; 2. cutting of the VD and vasovasostomy with absorbable sutures; 3. cutting and joining of the VD using absorbable sutures with an intraluminally situated lead fibre; and 4. cutting and joining of the VD using non-absorbable sutures with an intraluminally situated lead fibre. Ninety days after the surgery the VD was resected, patency and histopathological signs of inflammation in the VD were evaluated. Results: All vasovasostomy techniques were successfully performed in all animals using only the operating loupe. The patency rate was 100% in the subgroup with contusion. Differences in the patency rates were found among the subgroups with vasovasostomy (P=0.007). The patency rate was higher in the subgroup that underwent group 3. Compared with vasovasostomies, contusion was associated with lower rates of inflammation (P=0.02) and severe inflammation (P=0.003). No differences were found among the subgroups of vasovasostomy techniques.

Conclusion: Contusion of the VD was not related to impairment in terms of patency. Vasovasostomy with an intraluminally situated lead fibre resulted in the highest patency rate among the standard vasovasostomy techniques.

Keywords: vas deferens injury, inguinal herniotomy complication, operating loupe, vasovasostomy

Received: July 20, 2018; Accepted: October 24, 2018; Prepublished online: November 15, 2018; Published: December 11, 2019  Show citation

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Stichhauer, R., Koudelka, J., Ryska, A., Zivna, H., & Kaska, M. (2019). Various surgical techniques for the repair of injured vas deferens in rat experiments. Biomedical papers, 163(4), 335-340. doi: 10.5507/bp.2018.069
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