Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(2):141-146 | DOI: 10.5507/bp.2018.037

Identification of novel OXA-134-like β-lactamases in Acinetobacter lwoffii and Acinetobacter schindleri isolated from chicken litter

Patrik Mlynarcika, Jan Bardona,c, Miroslava Htoutou Sedlakovaa, Petra Prochazkovaa, Milan Kolara,b
a Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
b Institute of Molecular and Translation Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
c State Veterinary Institute Olomouc, Czech Republic

Background: Various food-producing animals have been recognized in recent years as a potential reservoir for the spread of antibiotic resistant bacteria that may pose a risk to human health and therefore their dissemination in the food production chain needs to be assessed.

Methods: In this study, 450 boot swabs from chicken farms were analyzed for the presence of antimicrobial resistance with a focus on β-lactams resistance in Acinetobacter species.

Results: Two β-lactamase-encoding genes were first time identified in Acinetobacter lwoffii and Acinetobacter schindleri isolates. The deduced amino acid sequence of OXA-496 shared 93.8% identity with OXA-363. The second OXA-134-like enzyme, OXA-537, had the highest sequence identity (97.8%) with OXA-235 and OXA-237.

Conclusions: The results of this study illustrate the occurrence of new OXA-134-like β-lactamases, called OXA-496 and OXA-537, carrying strains of Acinetobacter lwoffii and Acinetobacter schindleri in chicken farm litter, and highlight the possible role of Acinetobacter as a reservoir of resistance genes.

Keywords: antibiotics, chicken, β-lactamase, identification, resistance

Received: March 21, 2018; Accepted: June 22, 2018; Prepublished online: July 18, 2018; Published: June 25, 2019  Show citation

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Mlynarcik, P., Bardon, J., Htoutou Sedlakova, M., Prochazkova, P., & Kolar, M. (2019). Identification of novel OXA-134-like β-lactamases in Acinetobacter lwoffii and Acinetobacter schindleri isolated from chicken litter. Biomedical papers, 163(2), 141-146. doi: 10.5507/bp.2018.037
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