Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2014, 158(1):073-079 | DOI: 10.5507/bp.2012.010

Effect of progesterone-carbachol derivative on perfusion pressure and coronary resistance in isolated rat heart: via activation of the M2 muscarinic receptor

Lauro Figueroa-Valverdea, Francisco Diaz-Cedillob, Elodia Garcia-Cerveraa, Eduardo Pool Gomeza, Maria Lopez-Ramosa
a Laboratory of Pharmaco-Chemistry at the Faculty of Chemical Biological Sciences of the Universidad Autonoma de Campeche, Av. Agustin Melgar s/n, Col Buenavista C.P.24039 Campeche Cam., Mexico
b Escuela Nacional de Ciencias Biologicas del Instituto Politecnico Nacional. Prol. Carpio y Plan de Ayala s/n Col. Santo Tomas, Mexico, D.F. C.P. 11340

Aim: The present study was designed to investigate the effects of progesterone-carbachol derivative on perfusion pressure and coronary resistance in rats. An additional aim was to identify the molecular mechanisms involved.

Methods: The Langendorff model was used to measure perfusion pressure and coronary resistance changes in isolated rat heart after progesterone-carbachol derivative alone and after the following compounds; mifepristone (progesterone receptor blocker), yohimbine (α2 adreno-receptor antagonist), ICI 118,551 (selective β2 receptor blocker), atropine (non-selective muscarinic receptor antagonist), methoctramine (antagonist of M2 receptor) and L-NAME (inhibitor of nitric oxide synthase).

Results: The results show that progesterone-carbachol derivative [10-9 mM] significantly decreased perfusion pressure (P=0.005) and coronary resistance (P=0.006) in isolated rat heart. Additionally, the effect of progesterone-carbachol on perfusion pressure [10-9 to 10-4 mM] was only blocked in the presence of methoctramine and L-NAME.

Conclusions: These data suggest that progesterone derivative exert its effect on perfusion pressure via activation of the M2 muscarinic. In addition, this phenomenon involves stimulation of nitric oxide synthase (NOS).

Keywords: progesterone-carbachol, langendorff, perfusion pressure, coronary resistance

Received: July 22, 2011; Accepted: January 18, 2012; Prepublished online: January 31, 2012; Published: April 1, 2014  Show citation

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Figueroa-Valverde, L., Diaz-Cedillo, F., Garcia-Cervera, E., Gomez, E.P., & Lopez-Ramos, M. (2014). Effect of progesterone-carbachol derivative on perfusion pressure and coronary resistance in isolated rat heart: via activation of the M2 muscarinic receptor. Biomedical papers, 158(1), 073-079. doi: 10.5507/bp.2012.010
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