Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(2):227-233 | DOI: 10.5507/bp.2014.045

Biocompatibility of quantum dots (CdSe/ZnS ) in human amniotic membrane-derived mesenchymal stem cells in vitro

Gongping Wanga#, Guangwei Zengb#, Caie Wangc, Huasheng Wangd, Bo Yange, Fangxia Guanf, Dongpeng Lig, Xiaoshan Fengh
a Department of Oncological Surgery, the First Affiliated Hospital of Henan University of Science and Technology, LuoYang 471003 , Henan Province, PR China
b Department of Plastic, Reconstructive and Burns Surgery, the First Affiliated Hospital of Henan University of Science and Technology, LuoYang 471003 , Henan Province, PR China
c Department of Pharmacy, the First Affiliated Hospital of Henan University of Science and Technology, LuoYang 471003 , Henan Province, PR China
d Department of Colorectal Surgery, People Hospital of Zhengzhou, Zhengzhou 450000 , Henan Province, PR China
e Department of Neurosurgery, the First Affiliated Hospital of ZhengZhou University, ZhengZho 450001, Henan Province, PR China
f Henan Academy of Medical Sciences, ZhengZhou 450001, Henan Province, PR China
g Department of Emergency, the First Affiliated Hospital of Henan University of Science and Technology, LuoYang 471003 , Henan Province, PR China
h Department of Oncological Surgery, the First Affiliated Hospital of Henan University of Science and Technology, LuoYang 471003 , Henan Province, PR China

Background and Aim. Amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) are a potential source of mesenchymal stem cells which could be used to repair skin damage. The use of mesenchymal stem cells to repair skin damage requires safe, effective and biocompatible agents to evaluate the effectiveness of the result. Quantum dots (QDs) composed of CdSe/ZnS are semiconductor nanocrystals with broad excitation and narrow emission spectra, which have been considered as a new chemical and fluorescent substance for non-invasively labeling different cells in vitro and in vivo. This study investigated the cytotoxic effects of QDs on hAM-dMSCs at different times following labeling.

Methods: Using 0.75, 1.5 and 3.0 μL between quantum dots, labeled human amniotic mesenchymal stem cells were collected on days 1, 2 and 4 and observed morphological changes, performed an MTT cell growth assay and flow cytometry for mesenchymal stem cells molecular markers.

Results: Quantum dot concentration 0.75 μg/mL labeled under a fluorescence microscope, cell morphology was observed, The MTT assay showed cells in the proliferative phase. Flow cytometry expression CD29, CD31, CD34, CD44, CD90, CD105 and CD106.

Conclusions: Within a certain range of concentrations between quantum dots labeled human amniotic mesenchymal stem cells has good biocompatibility.

Keywords: quantum dots, biocompatibility, human amniotic membrane, mesenchymal stem cells

Received: September 24, 2013; Accepted: August 13, 2014; Prepublished online: October 2, 2014; Published: June 28, 2015  Show citation

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Wang, G., Zeng, G., Wang, C., Wang, H., Yang, B., Guan, F., Li, D., & Feng, X. (2015). Biocompatibility of quantum dots (CdSe/ZnS ) in human amniotic membrane-derived mesenchymal stem cells in vitro. Biomedical papers, 159(2), 227-233. doi: 10.5507/bp.2014.045
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