Biomedical papers, 2016 (vol. 160), issue 1

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016, 160(1):140-142 | 10.5507/bp.2015.056

The critical points in induction of experimental autoimmune uveitis

Aneta Klimovaa, Petra Seidler Stangovaa, Petra Svozilkovaa, John V. Forresterb,c, Izabela Klaskab, Jarmila Heissigerovaa
a Department of Ophthalmology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Czech Republic
b Section of Immunology and Infection, Institute of Medical Sciences, University of Aberdeen, United Kingdom
c Immunology and Virology Program, Centre for Ophthalmology and Visual Science, The University of Western Australia, Crawley, Western Australia, Australia and Centre for Experimental Immunology, Lions Eye Institute, Nedlands, Western Australia, Australia

Background: Autoimmune uveitis is a leading cause of visual impairment in developed countries in patients of working age. Animal models of experimental autoimmune uveitis (EAU) have been established to serve as a useful template for novel therapeutic approaches.

Methods: Experimental autoimmune uveitis is induced in C57BL/6 mice by subcutaneous application of interphotoreceptor retinoid binding protein in complete Freund's adjuvant and pertussis toxin. Clinical and histological grading is used to assess the inflammation intensity of EAU.

Results: The protocol of induction of EAU in mice hides several important aspects, which are crucial for developing the disease. These details have to be addressed to ensure reproducible disease induction. We describe our experience in establishing the model by pointing out the critical steps in EAU protocol which we found important.

Conclusion: The mouse model of EAU has practical value for preclinical studies, is robust and well established. However, the induction of inflammation of the eye can be quite challenging when important details of the protocol are not recognized and adhered to.

Keywords: experimental autoimmune uveitis, C57BL/6 mouse strain, mouse model, induction protocol, chronic posterior autoimmune uveitis

Received: May 16, 2015; Accepted: November 2, 2015; Prepublished online: November 10, 2015; Published: March 30, 2016


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