Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(3):388-393 | DOI: 10.5507/bp.2014.007

Effect of diabetic osteoblasts on osteogenic differentiation of human umbilical cord mesenchymal stem cells

Cheng-Zhi Haa, Hai-Ying Chenb, Juan Wangc, Wei Liub, Ying-Xin Zhangb, Li Panb, Wei-Hua Wangb, Shuang-Feng Chenb, Da-Wei Wanga,d, Le-Xin Wangd
a Department of Orthopedics, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China
b Oral Maxillofacial-Head and Neck Key Laboratory of Medical Biology, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China
c Department of Stomatology, Liaocheng People's Hospital, Liaocheng, Shandong, 252000, China
d School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW 2650, Australia

Background: This study was aimed to investigate whether osteoblasts from diabetic patients have a promoting effect on osteogenesis of human umbilical cord mesenchymal stem cells (HUMSCs).

Methods: HUMSCs were co-cultured with osteoblasts of diabetic and non-diabetic patients. Morphological appearance and cytochemical characteristics of the non-diabetic osteoblasts and diabetic osteoblasts were observed by hematoxylin-eosin staining, type I collagen protein expression, alkaline phosphatase (ALP) staining and Alizarin Red S staining. Cell viability, type I collagen protein expression, ALP activity and osteocalcin mRNA expression in HUMSCs were investigated.

Results: Compared with negative control group, the cell proliferation, type I collagen protein expression, ALP activity and osteocalcin mRNA were increased in HUMSCs co-cultured with diabetic and non-diabetic osteoblasts (P<0.05). There was no statistically significant difference in the HUMSCs cell proliferation, type I collagen protein expression, ALP activity and osteocalcin mRNA between the non-diabetic and diabetic group (P >0.05).

Conclusions: Similar to osteoblasts from non-diabetic patients, osteoblasts from diabetic patients also have the ability to promote HUMSCs proliferation, and leading to HUMSCs exhibit some characteristic of osteoblasts.

Keywords: diabetic, osteoblasts, mesenchymal stem cells, human umbilical cord, bone healing

Received: August 27, 2013; Accepted: January 22, 2014; Prepublished online: February 20, 2014; Published: September 30, 2015  Show citation

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Cheng-Zhi, H., Chen, H., Wang, J., Liu, W., Zhang, Y., Pan, L., ... Wang, L. (2015). Effect of diabetic osteoblasts on osteogenic differentiation of human umbilical cord mesenchymal stem cells. Biomedical papers, 159(3), 388-393. doi: 10.5507/bp.2014.007
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