Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2008, 152(2):231-233 | DOI: 10.5507/bp.2008.035

METHODOLOGICAL ASPECTS OF ATTEMPTS TO TRANS-DIFFERENTIATE ADULT STEM CELLS INTO EMBRYONIC-LIKE CELLS IN VITRO.

Petr Uhera, Petra Baborovaa, Renata Huttelovaa, Milena Kralickovaa,b, Pierre Vanderzwalmenc, Nicolas Zechc
a Institute of Reproductive Medicine and Endocrinology, B. Smetany 2, 30177 Pilsen, Czech Republic
b Department of Histology and Embryology, Faculty of Medicine in Plzen, Charles University in Prague, Karlovarskį 48, Plzeņ, Czech Republic, CZ-301 66,
c Centre Hospitalier Inter Regional Cavell (CHIREC), Braine l',à,lleud, Brussels, Belgium.

AIMS: The aim of this research was to set up an in vitro system to trans-differentiate haematopoietic stem cells (HSCs) into embryo-like stem cells in order to de-differentiate them. In this more naive state they should be cultivated more easily in order to augment them for consecutive differentiation and autologous transplantation for use in clinical practice.

METHODS: Using the principle of the methodology of blastocyst injection, HSCs were co-cultivated with mouse embryonic stem cells (mES) with and without cell to cell contact. After co-cultivation HSCs were analyzed by flow-cytometry using haematopoietic markers (CD34, CD45, CD133) and embryonic stem cell markers (SSEA-4, Tra-1-60, Tra-1-81).

RESULTS: No ES cell markers were detected on the former HSCs. A decrease in HSC marker intensity was the only finding. This implies that no de-differentiation took place.

CONCLUSIONS: We hypothesize that the unnatural situation of a mixture of two cell types originating in different species may have led to this outcome. To achieve our goal of in vitro de-differentiation we need to use a purely human culture system without animal additives.

Keywords: Haematopoietic stem cells, Mouse embryonic stem cells, Trans-diff erentiation, Regenerative medicine

Received: April 18, 2008; Accepted: May 10, 2008; Published: December 1, 2008  Show citation

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Uher, P., Baborova, P., Huttelova, R., Kralickova, M., Vanderzwalmen, P., & Zech, N. (2008). METHODOLOGICAL ASPECTS OF ATTEMPTS TO TRANS-DIFFERENTIATE ADULT STEM CELLS INTO EMBRYONIC-LIKE CELLS IN VITRO. Biomedical papers, 152(2), 231-233. doi: 10.5507/bp.2008.035
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