Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2008, 152(1):79-82 | DOI: 10.5507/bp.2008.012

CHANGES IN BIOMECHANICAL PARAMETERS DURING HEART PERFUSION AND AFTER MIDAZOLAM PRE-MEDICATION – EXPERIMENTAL PILOT STUDY

Lenka Bartosikovaa, Jiri Necasa, Tomas Bartosikb, Petr Franac, Martin Pavlikb
a Department of Physiology, Faculty of Medicine and Dentistry, Palacky University, 775 15 Olomouc, Czech Republic
b Department of Anaesthesiology and Intensive Care, St. Anne',s University Hospital Brno, Czech Republic
c 2nd Department of Internal Medicine, St. Anne',s University Hospital Brno, Czech Republic

Background: Midazolam is a frequently used benzodiazepine in anaesthesiology and intensive care.

Aim: The aim of pilot study was to monitor its effect during heart perfusion in the laboratory rat.

Methods: The same groups of animals (n = 10). The 1st group was treated with midazolam in a dose of 0.5mg/kg i.p. The 2nd group was a placebo. After i.p. administration of heparine injection of 500 IU dose, the hearts were excised and perfused (modified Langendorf's method). Working schedule: stabilization/ischaemia/reperfusion proceed at intervals of 20/30/60 min. Monitored parameters in isolated heart: left ventricle pressure (LVP), end-diastolic pressure (LVEDP), contractility (+dP/dtmax).

Results: The treated hearts showed improved postischemic recovery, reaching LVP values of 92 ± 6 % at the end of the reperfusion, placebo only 61 ± 7 %. In placebo hearts LVEDP rose from 10.0 ± 0.5 mmHg to 43 ± 4 mmHg after, in treated animals only about 25 mmHg. The treated hearts improved +dP/dtmax recovery during reperfusion to 91 ± 8 %. These values were significantly greater than those obtained from the placebo hearts.

Conclusions: Positive changes in monitored parameters were found in this experimental pilot study. We conclude that the administration of midazolam in laboratory rats has a cardioprotective potential against ischemia-reperfusion induced injury.

Keywords: Heart ischemia-reperfusion, Midazolam

Received: February 15, 2008; Accepted: April 25, 2008; Published: June 1, 2008  Show citation

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Bartosikova, L., Necas, J., Bartosik, T., Frana, P., & Pavlik, M. (2008). CHANGES IN BIOMECHANICAL PARAMETERS DURING HEART PERFUSION AND AFTER MIDAZOLAM PRE-MEDICATION – EXPERIMENTAL PILOT STUDY. Biomedical papers, 152(1), 79-82. doi: 10.5507/bp.2008.012
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