Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2025, 169(2):73-81 | DOI: 10.5507/bp.2024.033

Adrenal insufficiency - causes and laboratory diagnosis

Tomas Brutvan1, Jana Jezkova1, Marcela Kotasova2, Michal Krsek1
1 Third Department of Internal Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic
2 Institute of Clinical Biochemistry and Laboratory Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic

Adrenal insufficiency (AI) manifests as a clinical syndrome arising from either the direct impairment of adrenal glands, leading to primary AI characterized by deficiencies in glucocorticoids and mineralocorticoids, or adrenal cortex atrophy due to diminished adrenocorticotropic hormone (ACTH) stimulation, a consequence of hypothalamic and/or pituitary damage, resulting in secondary AI. The diagnosis of AI is based on clinical assessment and biochemical tests, including basal hormone level measurements and stimulation tests. In evaluating the results of laboratory tests, it is necessary to consider factors that may influence both pre-analytical and analytical phases, as well as the chosen methodology. Correct diagnosis of adrenal insufficiency and timely initiation of suitable replacement therapy are paramount. These steps are crucial not only for managing the condition but also to avert potentially life-threatening adrenal crises.

Keywords: adrenal insufficiency, cortisol, adrenocorticotropic hormone, stimulation test

Received: June 26, 2024; Revised: September 16, 2024; Accepted: September 30, 2024; Prepublished online: October 7, 2024; Published: June 1, 2025  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Brutvan, T., Jezkova, J., Kotasova, M., & Krsek, M. (2025). Adrenal insufficiency - causes and laboratory diagnosis. Biomedical papers169(2), 73-81. doi: 10.5507/bp.2024.033
Download citation

References

  1. . Arlt W, Allolio B. Adrenal insufficiency. Lancet 2003;361(9372):1881-93. Go to original source... Go to PubMed...
  2. . Regal M, Páramo C, Sierra SM, Garcia-Mayor RV. Prevalence and incidence of hypopituitarism in an adult Caucasian population in northwestern Spain. Clin Endocrinol (Oxf) 2001;55(6):735-40. Go to original source... Go to PubMed...
  3. . Lovas K, Husebye ES. High prevalence and increasing incidence of Addison's disease in western Norway. Clin Endocrinol 2002;56(6):787-9. Go to original source... Go to PubMed...
  4. . Betterle C, Volpato M, Pedini B, Chen S, Smith BR, Furmaniak J. Adrenal-cortex autoantibodies and steroid-producing cells autoantibodies in patients Addison's disease: comparison of immunofluorescence and immunoprecipitation assays. J Clin Endocrinol Metab 1999;84:618-22. Go to original source... Go to PubMed...
  5. . Betterle C, Dal Pra C, Mantero F, Zanchetta R. Autoimmune adrenal insufficiency and autoimmune polyendocrine syndromes: autoantibodies, autoantigens, and their applicability in diagnosis and disease prediction. Endocr Rev 2002;23:327-64. Go to original source... Go to PubMed...
  6. . Neufeld M, Maclaren NK, Blizzard RM. Two types of autoimmune Addison´s disease associated with different polyglandular autoimmune (PGA) syndromes. Medicine (Baltimor) 1981;60:355-62. Go to original source... Go to PubMed...
  7. . Ahonen P, Myllarniemi S, Sipila I, Perheentupa J. Clinical variation of autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) in a series of 68 patients. N Engl J Med 1990;322:1829-36. Go to original source... Go to PubMed...
  8. . Perheentupa J. Autoimmune polyendocrinopathy- candidiasis-ectodermal dystrophy. J Clin Endocrinol Metab 2006;91:2843-50. Go to original source... Go to PubMed...
  9. . Husebye ES, Anderson MS, Kämpe O. Autoimmune Polyendocrine Syndromes. N Engl J Med 2018;378(12):1132-41. Go to original source... Go to PubMed...
  10. . Chen S, Sawicka J, Betterle C, Powell M, Prentice L, Volpato M, Rees Smith B, Furmaniak J. Autoantibodies to steroidogenic enzymes in autoimmune polyglandular syndrome, Addison's disease, and premature ovarian failure. J Clin Endocrinol Metab 1996;81(5):1871-6. Go to original source... Go to PubMed...
  11. . Naletto L, Frigo ACh, Ceccato F. The natural history of autoimmune Addison's disease from the detection of autoantibodies to development of the disease: a long-term follow-up study on 143 patients Eur J Endocrinol 2019;180(3):223-34. Go to original source... Go to PubMed...
  12. . Krone N, Dhir V, Ivison HE, Artl W. Congenital adrenal hyperplasia and P450 oxidoreductase deficiency. Clin Endocrinol 2007;66(2):162-72. Go to original source... Go to PubMed...
  13. . Neocleous V, Fanis P, Phylactou LA, Skordis N. Genotype Is Associated to the Degree of Virilization in Patients With Classic Congenital Adrenal Hyperplasia. Front Endocrinol (Lausanne) 2018;9:733. Go to original source... Go to PubMed...
  14. . White PC, Speiser PW. Congenital adrenal hyperplasia due to 21 - hydoxylase deficiency. Endocr Rev 2000;21(3):245-91. Go to original source... Go to PubMed...
  15. . Mosser J, Douar AM, Sarde CO, Kioschis P, Feil R, Moser H, Poustka AM, Mandel JL, Aubourg P. Putative X-linked adrenoleukodystrophy gene shares unexpected homology with ABC transporters. Nature 1993;361(6414):726-30. Go to original source... Go to PubMed...
  16. . Mosser J, Lutz Y, Stoeckel ME, Sarde CO, Kretz C, Douar AM, Lopez J, Aubourg P, Mandel JL. The gene responsible for adrenoleukodystrophy encodes a peroxisomal membrane protein. Hum Mol Genet 1994;3(2):265-71. Go to original source... Go to PubMed...
  17. . Moser HW. Adrenoleukodystrophy: phenotype, genetics, pathogenesis and therapy. Brain 1997;120:1485-508. Go to original source... Go to PubMed...
  18. . Miller WL. Congenital lipoid adrenal hyperplasia: the human gene knockout for the steroidogenic acute regulatory protein. J Mol Endocrinol 1997;19(3):227-40. Go to original source... Go to PubMed...
  19. . Miller WL. Androgen biosynthesis from cholesterol to DHEA. Mol Cell Endocrinol 2002;198(1-2):7-14. Go to original source... Go to PubMed...
  20. . Peter M, Viemann M, Partsch CJ, Sippell WG, et al. Congenital Adrenal Hypoplasia: Clinical Spectrum, Experience with Hormonal Diagnosis, and Report on New Point Mutations of the DAX-1 Gene. J Clin Endocrinol Metab 1998;8(1):2666-74. Go to original source... Go to PubMed...
  21. . Suntharalingham JP, Buonocore F, Duncan AJ, Achermann JC. DAX-1 (NR0B1) and steroidogenic factor-1 (SF-1, NR5A1) in human disease. Best Pract Res Clin Endocrinol Metab 2015;29(4):607-19. Go to original source... Go to PubMed...
  22. . Meimaridou E, Hughes CR, Kowalczyk J, Chan LF, Clark AJ, Metherell LA. ACTH resistance: genes and mechanisms. Endocr Dev 2013;24:57-66. Go to original source... Go to PubMed...
  23. . Dumic M, Bari¹ic N, Kusec V, Stingl K, Skegro M, Stanimirovic A, Koehler K, Huebner A. Long-term clinical follow-up and molecular genetic findings in eight patients with triple A syndrome. Eur J Pediatr 2012;171(10):1453-9. Go to original source... Go to PubMed...
  24. . Guran T, Buonocore F, Saka N. Rare Causes of Primary Adrenal Insufficiency: Genetic and Clinical Characterization of a Large Nationwide Cohort. J Clin Endocrinol Metab 2016;101(1):284-92. Go to original source... Go to PubMed...
  25. . Piccioli A, Chini G, Mannelli M. Bilateral massive adrenal hemorrhage due to sepsis: report of two cases. J Endocrinol Invest 1994;170(10):821-4. Go to original source... Go to PubMed...
  26. . Castinetti F, Nieman LK, Reincke. Approach to the Patient Treated with Steroidogenesis Inhibitors. J Clin Endocrinol Metab 2021;106(7):2114-23. Go to original source... Go to PubMed...
  27. . Gray S, Bliski T, Dieudonne B. Hypopituitarism After Traumatic Brain Injury. Cureus 2019;11(3):e4163. Go to original source... Go to PubMed...
  28. . Mody S, Brown MR, Parks JSl. The spectrum of hypopituitarism caused by PROP1 mutations. Best Pract Res Clin Endocrinol Metab 2002;16(3):421-31. Go to original source... Go to PubMed...
  29. . Baker EH. Is there a safe and effective way to wean patients off long-term glucocorticoids? Br J Clin Pharmacol 2021;87(1):12-22. Go to original source... Go to PubMed...
  30. . Sannarangappa V, Jalleh R. Inhaled Corticosteroids and Secondary Adrenal Insufficiency. Open Respir Med J 2014;8:93-100. Go to original source... Go to PubMed...
  31. . González Villarroel P, Fernández Pérez I, Páramo C, Gentil González M, Carnero López B, Vázquez Tuñas ML, Carrasco Alvarez JA. Megestrol acetate-induced adrenal insufficiency. Clin Transl Oncol 2008;10(4):235-7. Go to original source... Go to PubMed...
  32. . Dux S, Bishara J, Marom D, Blum I, Pitlik S. Medroxyprogestreone acetate induced secondary adrenal insufficiency. Ann Pharmacother 1998;32(1):134. Go to original source... Go to PubMed...
  33. . Li T, Cunningham Jl, Gilliam WP, Loukianova L, Donegan DM. Bancos I. Prevalence of Opioid-Induced Adrenal Insufficiency in Patients Taking Chronic Opioids J Clin Endocrinol Metab 2020;105(10):e3766-e3775. Go to original source... Go to PubMed...
  34. . Oelkers W. Hyponatremia and inappropriate secretion of vasopressin (antidiuretic hormone) in patients with hypopituitarism. N Engl J Med 1989;321(8):492-6. Go to original source... Go to PubMed...
  35. . Mitchell AL, Pearce SH. Autoimmune Addison disease: pathophysiology and genetic complexity. Nat Rev Endocrinol 2012;8:306-16. Go to original source... Go to PubMed...
  36. . Papierska L, Rabijewski M. Delay in diagnosis of adrenal insufficiency is a frequent cause of adrenal crisis. Int J Endocrinol 2013;2013:482370. Go to original source... Go to PubMed...
  37. . Husebye ES, Allolio B, Arlt W, Badenhoop K, Bensing S, Betterle C, Falorni A, Gan EH, Hulting AL, Kasperlik-Zaluska A, Kämpe O, Løvås K, Meyer G, Pearce SH. Consensus statement on the diagnosis, treatment and follow-up of patients with primary adrenal insufficiency. J Intern Med 2014;275(2):104-15. Go to original source... Go to PubMed...
  38. . Puar TH, Stikkelbroeck NM, Smans LC, Zelissen PM, Hermus AR. Adrenal crisis: still a deadly event in the 21st century. Am J Med 2016; 129(3):339.e1-9. doi: 10.1016/j.amjmed.2015.08.021 Go to original source... Go to PubMed...
  39. . Arlt W, Callies F, van Vlijmen JC, Koehler I, Reincke M, Bidlingmaier M, Huebler D, Oettel M, Ernst M, Schulte HM, Allolio B. Dehydroepiandrosterone replacement in women with adrenal insufficiency. N Engl J Med 1999;341(14):1013-20. Go to original source... Go to PubMed...
  40. . Nerup J. Addison's disease - clinical studies. A report for 108 cases. Acta Endocrinol (Copenh) 1974;76(1):127-41. Go to original source... Go to PubMed...
  41. . Minisola S, Pepe J, Piemonte S, Cipriani C. The diagnosis and management of hypercalcaemia. BMJ 2015;350:h2723. Go to original source... Go to PubMed...
  42. . Kato A, Shinozaki S, Goga T, Hishida A. Isolated adrenocorticotropic hormone deficiency presenting with hypercalcemia in a patient on long-term hemodialysis. Am J Kidney Dis 2003;42(2):E32-E36. Go to original source... Go to PubMed...
  43. . Chanson P. Severe hyponatremia as a frequent revealing sign of hypopituitarism after 60 years of age. Eur J Endocrinol 2003;149:177-8. Go to original source... Go to PubMed...
  44. . Selmaoui B, Touitou Y. Reproducibility of the circadian rhythms of serum cortisol and melatonin in healthy subjects: a study of three different 24-h cycles over six weeks. Life Sci 2003;73(26):3339-49. Go to original source... Go to PubMed...
  45. . Verbeeten KC, Ahmet AH. The role of corticosteroid-binding globulin in the evaluation of adrenal insufficiency. J Pediatr Endocrinol Metab 2018;31(2):107-15. Go to original source... Go to PubMed...
  46. . Hägg E, Asplund K, Lithner F. Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency. Clin Endocrinol 1987(2);26:221-6. Go to original source... Go to PubMed...
  47. . Schmidt IL, Lahner H, Mann K, Petersenn S. Diagnosis of adrenal insufficiency: evaluation of the corticotropin-releasing hormone test and basal serum cortisol comparison to the insulin tolerance test in patients with hypothalamic-pituitary-adrenal gland disease. J Clin Endocrinol Metab 2003;88(9):4193-8. Go to original source... Go to PubMed...
  48. . Erturk E, Jaffe CA, Barkan AL. Evaluation of the integrity of the hypothalamic-pituitary-adrenal axis by insulin hypoglycemia test. J Clin Endocrinol Metab 1998;83:2350-4. Go to original source...
  49. . Kazlauskaite R, Evans AT, Villabona CV, Abdu TA, Ambrosi B, Atkinson AB, Choi CH, Clayton RN, Courtney CH, Gonc EN, Maghnie M, Rose SR, Soule SG, Tordjman K; Consortium for Evaluation of Corticotropin Test in Hypothalamic-Pituitary Adrenal Insufficiency. Corticotropin tests for hypothalamic-pituitary- adrenal insufficiency: a metaanalysis. J Clin Endocrinol Metab 2008;93(11):4245-53. Go to original source... Go to PubMed...
  50. . Gatti R, Antonelli G, Prearo M, Spinella P, Cappellin E, De Palo EF. Cortisol assays and diagnostic laboratory procedures in human biological fluids. Clin Biochem 2009;42(12):1205-17. Go to original source... Go to PubMed...
  51. . Hawley JM, Owen LJ, MacKenzie F, Mussell C, Cowen S, Keevil BG. Candidate Reference Measurement Procedure for the Quantification of Total Serum Cortisol with LC-MS/MS. Clin Chem 2016;62(1):262-9. Go to original source... Go to PubMed...
  52. . Kline GA, Buse J, Krause RD. Clinical implications for biochemical diagnostic threshold of adrenal sufficiency using a highly specific cortisol immunoassay. Clin Biochem 2017;50(9):475-80. Go to original source... Go to PubMed...
  53. . Vogeser M, Kratzsch J, Ju Bae Y, Bruegel M, Ceglarek U, Fiers T, Gaudl A, Kurka H, Milczynski C, Prat Knoll C, Suhr AC, Teupser D, Zahn I, Ostlund RE. Multicenter performance evaluation of a second generation cortisol assay. Clin Chem Lab Med 2017;55(6):826-35. Go to original source... Go to PubMed...
  54. . Grassi G, Morelli V, Ceriotti F, Polledri E, Fustinoni S, D'Agostino S, Mantovani G, Chiodini I, Arosio M. Minding the gap between cortisol levels measured with second-generation assays and current diagnostic thresholds for the diagnosis of adrenal insufficiency: a single-center experience. Hormones (Athens) 2020;19(3):425-31. Go to original source... Go to PubMed...
  55. . Hawley JM, Owen LJ, Lockhart SJ, Monaghan PJ, Armston A, Chadwick CA, Wilshaw H, Freire M, Perry L, Keevil BG. Serum Cortisol: An Up-To-Date Assessment of Routine Assay Performance. Clin Chem 2016;62(9):1220-9. Go to original source... Go to PubMed...
  56. . Keevil BG. LC-MS/MS analysis of steroids in the clinical laboratory. Clin Biochem 2016;49 (13-14):989-97. Go to original source... Go to PubMed...
  57. . Monaghan PJ, Keevil BG, Stewart PM, Trainer PJ. Case for the wider adoption of mass spectrometry-based adrenal steroid testing, and beyond. J Clin Endocrinol Metab 2014;99 (12):4434-7. Go to original source... Go to PubMed...
  58. . Lee MK, Vasikaran S, Doery JC, Wijeratne N, Prentice D. Cortisol: ACTH ratio to test for primary hypoadrenalism: a pilot study. Postgrad Med J 2013;89:617-20. Go to original source... Go to PubMed...
  59. . Peccori GF, Saccani A, Cavagnini F. Assessment of ACTH assay variability: a multicenter study. Eur J Endocrinol 2011;164(4):505-12. Go to original source... Go to PubMed...
  60. . Bornstein SR, Allolio B, Arlt W, Barthel A, Don-Wauchope A, Hammer GD, Husebye ES, Merke DP, Murad MH, Stratakis CA, Torpy DJ. Diagnosis and Treatment of Primary Adrenal Insufficiency: An Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab 2016;101(2):364-89. Go to original source... Go to PubMed...
  61. . Saenger P, Levine LS, Irvine WJ, Gottesdiener K, Rauh W, Sonino N, Chow D, New MI. Progressive adrenal failure in polyglandular autoimmune disease. J Clin Endocrinol Metab 1982;54(4):863-7. Go to original source... Go to PubMed...
  62. . Dorn LD, Lucke JF, Loucks TL, Berga SL. Salivary cortisol reflects serum cortisol: analysis of circadian profiles. Ann Clin Biochem 2007;44:281-4. Go to original source... Go to PubMed...
  63. . Gröschl M. Current status of salivary hormone analysis. Clin Chem 2008;54(11):1759-69. Go to original source... Go to PubMed...
  64. . Perogamvros I, Keevil BG, Ray DW, Trainer PJ. Salivary cortisone is a potential biomarker for serum free cortisol. J Clin Endocrinol Metab 2010;95(11):4951-8. Go to original source... Go to PubMed...
  65. . Jonsson BAG, Malmberg B, Amilon A, Garde AH. Determination of cortisol in human saliva using chromatrography-electrospray tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 2003;784(1):63-8. Go to original source... Go to PubMed...
  66. . Perogamvros I, Owen LJ, Newell-Price J, Ray DW, Trainer PJ, Keevil BG. Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography-tandem mass spectrometry: application in basal and stimulated conditions. J Chromatogr B Analyt Technol Biomed Life Sci 2009;877(29):3771-5. Go to original source... Go to PubMed...
  67. . Deutschbein T, Broecker-Preuss M, Flitsch J, Jaeger A, Althoff R, Walz MK, Mann K, Petersenn S. Salivary cortisol as a diagnostic tool for Cushing's syndrome and adrenal insufficiency: improved screening by an automatic immunoassay. Eur J Endocrinol 2012;166(4):613-8. Go to original source... Go to PubMed...
  68. . Snow K, Jiang NS, Kao PC, Scheithauer BW. Biochemical evaluation of adrenal dysfunction: the laboratory perspective. Mayo Clin Proc 1992;67(11):1055-65. Go to original source... Go to PubMed...
  69. . de Miguel Novoa P, Vela ET, García NP, Rodríguez MM, Guerras IS, Martínez de Salinas Santamaría Mde L, Masó AA; Área de Conocimiento del Grupo de Neuroendocrino de la SEEN (Grupo Insuficiencia Adrenal). Guidelines for the diagnosis and treatment of adrenal insufficiency in the adult. Endocrinol Nutr 2014;61Suppl1:1-35. doi: 10.1016/S1575-0922(14)73526-0 (In English, Spanish) Go to original source... Go to PubMed...
  70. . Kosak M, Duskova M, Starka L, Jandikova H, Pospisilova H, Sramkova M, Hana V, Krsek M, Springer D, Simunkova K. Can the gold standard be beaten? How reliable are various modifications of the Synacthen test compared to the insulin tolerance test. Physiol Res 2017;66(Suppl 3):S387-S395. Go to original source... Go to PubMed...
  71. . Nye EJ, Grice JE, Hockings GI, Strakosch CR, Crosbie GV, Walters MM, Jackson RV. Comparison of adrenocorticotropin (ACTH) stimulation tests and insulin hypoglycemia in normal humans: low dose, standard high dose, and 8-hour ACTH-(1-24) infusion tests. J Clin Endocrinol Metab 1999;84(10):3648-55. Go to original source... Go to PubMed...
  72. . Patel L, Clayton PE. Clinical usefulness of the low dose ACTH test. J Endocrinol Invest 1999;22:401-4. Go to original source... Go to PubMed...
  73. . Rasmuson S, Olsson T, Hagg E. A low dose ACTH test to assess the function of the hypothalamic-pituitary-adrenal axis. Clin Endocrinol (Oxf) 1996:44(2):151-6. Go to original source... Go to PubMed...
  74. . Ospina NS, Al Nofal A, Bancos I, Javed A, Benkhadra K, Kapoor E, Lteif AN, Natt N, Murad MH. ACTH Stimulation Tests for the Diagnosis of Adrenal Insufficiency: Systematic Review and Meta-Analysis. J Clin Endocrinol Metab 2016;101(2):427-34. Go to original source... Go to PubMed...
  75. . Dekkers OM, Timmermans JM, Smit JW, Romijn JA, Pereira AM. Comparison of the cortisol responses to testing with two doses of ACTH in patients with suspected adrenal insufficiency. Eur J Endocrinol 2011;164(1):83-7. Go to original source... Go to PubMed...
  76. . Jones SL, Trainer PJ, Perry L, Wass JA, Bessser GM, Grossman A. An audit of the insulin tolerance test in adult subjects in an acute investigation unit over one year. Clin Endocrinol (Oxf) 1994;41(1):123-8. Go to original source... Go to PubMed...
  77. . Finucane FM, Liew A, Thornton E, Rogers B, Tormey W, Agha A. Clinical insights into the safety and utility of the insulin tolerance test (ITT) in the assessment of the hypothalamo-pituitary-adrenal axis. Clin Endocrinol (Oxf) 2008;69(4):603-7. Go to original source... Go to PubMed...
  78. . Lee P, Greenfield JR, Ho KKY. Factors determining inadequate hypoglycaemia during insulin tolerance testing (ITT) after pituitary surgery. Clin Endocrinol (Oxf) 2009;71(1):82-5. Go to original source... Go to PubMed...
  79. . Fish HR, Chernow B, O'Brian JT. Endocrine and neurophysiologic responses of the pituitary to insulin-induced hypoglycemia: a review. Metabolism 1986;35(8):763-80. Go to original source... Go to PubMed...
  80. . Hurel SJ, Thompson CJ, Watson MJ, Harris MM, Baylis PH, Kendall-Taylor P. The short Synacthen and insulin stress tests in the assessment of the hypothalamic-pituitary-adrenal axis. Clin Endocrinol 1996;44(2):141-6. Go to original source... Go to PubMed...
  81. . Cho HY, Kim JH, Kim SW, Shin CS, Park KS, Yang HC, Kim SY. Different cut-off values of the insulin tolerance test, the high-dose short Synacthen test (250μg) and the low-dose short Synacthen test (1μg/) in assessing central adrenal insufficiency. Clin Endocrinol 2014;81(1):77-84. Go to original source... Go to PubMed...
  82. . Fleseriu M, Hashim IA, Karavitaki N, Melmed S, Murad MH, Salvatori R, Samuels MH. Hormonal Replacement in Hypopituitarism in Adults: An Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab 2016;101(11):3888-921. Go to original source... Go to PubMed...
  83. . Berg Ch, Meinel T, Lahner H, Yuece A, Mann K, Petersenn S. Diagnostic utility of the glucagon stimulation test in comparison to the insulin tolerance test in patients following pituitary surgery. Eur J Endocrinol 2010;162(3):477-82. Go to original source... Go to PubMed...
  84. . Ach T, Yosra H, Jihen M, Abdelkarim Asma B, Maha K, Molka C, Rouatbi S, Monia Z, Ach K. Cortisol cut-points for the glucagon stimulation test in the evaluation of hypothalamic pituitary adrenal axis. Endocr J 2018;65(9):935-42. Go to original source... Go to PubMed...
  85. . Hamrahian AH, Yuen KC, Gordon MB, Pulaski-Liebert KJ, Bena J, Biller BM. Revised GH and cortisol cut-points for the glucagon stimulation test in the evaluation of GH and hypothalamic-pituitary-adrenal axes in adults: Results from a prospective randomized multicenter study. Pituitary 2016;19,332-41. Go to original source... Go to PubMed...
  86. . Cousins L, Rigg L, Hollongsworth D, Meis P, Halberg F, Brink G, Yen SS. Qualitative and quantitative assessment of the circadian rhythm of cortisol in pregnancy. Am J Obstet Gynecol 1983;145(4):411-6. Go to original source... Go to PubMed...
  87. . Jung C, Ho JT, Torpy DJ, Rogers A, Doogue M, Lewis JG, Czajko RJ, Inder WJ. A longitudinal study of plasma and urinary cortisol in pregnancy and postpartum. J Clin Endocrinol Metab 2011;96(5):1533-40. Go to original source... Go to PubMed...
  88. . Bjornsdottir S, Cnattingius S, Brandt L, Nordenstrom A, Ekbom O, Kampe O, Bensing S. Addison's disease in women is a risk factor for an adverse pregnancy outcome. J Clin Endocrinol Meteb 2010;95(12):5249-57. Go to original source... Go to PubMed...
  89. . Lebbe M, Arlt W. What is the best diagnostic and therapeutic management strategy for an Addison patient during pregnancy? Clin Endocrinol 2013;88(4):497-502. Go to original source... Go to PubMed...
  90. . Maier B, Vogeser M. Target analyte quantification by isotope dilution LC-MS/MS directly referring to internal standard concentrations-validation for serum cortisol measurement. Clin Chem Lab Med 2013;51(4):833-7. Go to original source... Go to PubMed...
  91. . Speiser PW, Azziz R, Baskin LS, Ghizzoni L, Hensle TW, Merke DP, Meyer-Bahlburg HF, Miller WL, Montori VM, Oberfield SE, Ritzen M, White PC; Endocrine Society. Congenital adrenal hyperplasia due to steroid 21-hydroxylase deficiency: an Endocrine Society clinical practice guideline. J Clin Endocrinol Metab 2010;95(9):4133-60. doi: 10.1210/jc.2009-2631. Erratum in: J Clin Endocrinol Metab 2010;95(11):5137. Erratum in: J Clin Endocrinol Metab 2021;106(7):e2853. Go to original source... Go to PubMed...
  92. . Baumgartner-Parzer SM, Nowotny P, Heinze G, Waldhäusl W, Vierhapper H. Carrier frequency of congenital adrenal hyperplasia (21-hydroxylase deficiency) in a middle European population. J Clin Endocrinol Metab 2005;90(2):775-8. Go to original source... Go to PubMed...
  93. . Sabina Baumgartner-Parzer S, Witsch-Baumgartner M, Hoeppner W. EMQN best practice guidelines for molecular genetic testing and reporting of 21-hydroxylase deficiency. EJHG 2020;28:1341-67. Go to original source... Go to PubMed...
  94. . Owen LJ, Halsall DJ, Keevil BG. Cortisol measurement in patients receiving metyrapone therapy. Ann Clin Biochem 2010;47:573-5. Go to original source... Go to PubMed...

This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.