Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(2):131-138 | DOI: 10.5507/bp.2021.055

Combination of plant phenolics and isoquinolinium alkaloids protects gingival fibroblast and improves post-extraction healing after lower third molar extraction

Karin Chytilova1, Adela Galandakova2, Katerina Valentova2, 3, Peter Tvrdy1
1 Department of Oral and Maxillofacial Surgery, University Hospital, I. P. Pavlova 6, 779 00 Olomouc, Czech Republic
2 Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
3 Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic

Aims: The effect of polyphenolic fraction of Lonicera caerulea (PFLC) and alkaloid fraction of Macleaya cordata (AFMC) mix on the production of inflammatory mediators in human gingival fibroblasts pretreated with lipopolysaccharide (LPS) was investigated. In addition, protective effects of mucoadhesive paste containing combination of PFLC and AFMC (0.05% and 0.01%, respectively; n=15, Group A) and placebo (n=15, Group B) were evaluated in patients after surgical extraction of lower third molars.

Methods: Gingival fibroblasts were pre-treated with LPS (10 µg/mL; 24 h) and PFLC/AFMC (25/0.25; 50/0.25; 100/0.25; 25/0.5; 50/0.5; 100/0.5 µg/mL) in serum-free medium was applied for 4 h. Then the interleukin-6 (IL-6), reactive oxygen species (ROS) generation, level of intracellular glutathione (GSH) and expression of cyclooxygenase-2 (COX-2) were evaluated. The study was a 6-day, single-center, randomized, double-blind and placebo-controlled trial consisting of two parallel treatment arms. A modified Oral health impact profile questionnaire including both general oral condition and extraction related questions, was used to evaluate the oral condition and other changes before (day 0) and on the days 1, 3 and 6 after surgical extraction.

Results and Conclusion: The combination of PFLC with AFMC caused a reduction of ROS generation, reduced IL-6 production and suppressed the expression of COX-2. In group A the paste treatment contributed to improvement of oral health-related quality of life. Topical application of PFLC and AFMC into the extraction wound improved post-extraction site wound healing probably by antioxidant and anti-inflammatory mechanisms.

Keywords: Lonicera caerulea, Macleaya cordata, anthocyanins, isoquinolinium alkaloids, gingival fibroblast, third molar surgery

Received: August 6, 2021; Revised: August 6, 2021; Accepted: September 17, 2021; Prepublished online: October 6, 2021; Published: June 12, 2023  Show citation

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Chytilova, K., Galandakova, A., Valentova, K., & Tvrdy, P. (2023). Combination of plant phenolics and isoquinolinium alkaloids protects gingival fibroblast and improves post-extraction healing after lower third molar extraction. Biomedical papers, 167(2), 131-138. doi: 10.5507/bp.2021.055
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