Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(1):71-76 | DOI: 10.5507/bp.2018.079

The absence of high-risk human papillomavirus in Czech non-small cell lung cancer cases

Hana Jaworek, Vladimira Koudelakova, Rastislav Slavkovsky, Jiri Drabek, Marian Hajduch
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 1333/5, 779 00 Olomouc, Czech Republic
Corresponding author: Vladimira Koudelakova, e-mail: vladimira.koudelakova@upol.cz

Aims: The purpose of our study was to examine the presence of human papillomavirus (HPV) DNA in Czech patients with non-small cell lung cancer (NSCLC). Methods: A highly sensitive quantitative polymerase chain reaction (qPCR) detecting the E6 gene of HPV16, 18, 31, and 56 was designed. The limit of detection was assessed using serial dilutions of HPV-positive plasmids. The qPCR was validated on a set of 402 cervical swabs where the qPCR, Cobas, and PapilloCheck methods were tested in parallel. Finally, qPCR was used for HPV detection in a set of 80 patients with primary NSCLC, both from formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples.

Results: The qPCR method was able to reliably detect at least 4 copies of the E6 gene per reaction in HPV16, 18, and 31, and 40 copies per reaction in HPV56. The sensitivity and specificity of the qPCR were 75.6-99.3% and 63.9-100% respectively, depending on the HPV genotype and reference method used. HPV DNA was not detected in the FFPE and FF samples from the set of 80 NSCLC patients.

Conclusion: No hrHPV DNA was found in primary NSCLC tumors from a Czech population.

Keywords: human papillomavirus, non-small cell lung cancer, PCR, HPV16, HPV18

Received: August 23, 2018; Accepted: December 11, 2018; Prepublished online: January 10, 2019; Published: March 26, 2020  Show citation

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Jaworek, H., Koudelakova, V., Slavkovsky, R., Drabek, J., & Hajduch, M. (2020). The absence of high-risk human papillomavirus in Czech non-small cell lung cancer cases. Biomedical papers, 164(1), 71-76. doi: 10.5507/bp.2018.079
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    References

    1. Spyratos D, Zarogoulidis P, Porpodis K, Tsakiridis K, Machairiotis N, Katsikogiannis N, Kougioumtzi I, Dryllis G, Kallianos A, Rapti A, Li C, Zarogoulidis K. Occupational exposure and lung cancer. J Thorac Dis 2013;5 Suppl 4:S440-S445. Go to PubMed...
    2. Moore PS, Chang Y. Why do viruses cause cancer? Highlights of the first century of human tumour virology. Nat Rev Cancer 2010;10(12):878-89. Go to original source... Go to PubMed...
    3. Gao G, Smith DI. Human Papillomavirus and the Development of Different Cancers. Cytogenet Genome Res 2016;150(3-4):185-93. Go to original source... Go to PubMed...
    4. Leemans CR, Snijders PJF, Brakenhoff RH. The molecular landscape of head and neck cancer. Nat Rev Cancer 2018;18(5):269-82. Go to original source... Go to PubMed...
    5. Katsenos S, Becker HD. Recurrent respiratory papillomatosis: a rare chronic disease, difficult to treat, with potential to lung cancer transformation: apropos of two cases and a brief literature review. Case Rep Oncol 2011;4(1):162-71. Go to original source... Go to PubMed...
    6. Carifi M, Napolitano D, Morandi M, Dall'Olio D. Recurrent respiratory papillomatosis: current and future perspectives. Ther Clin Risk Manag 2015;11:731-8. Go to original source... Go to PubMed...
    7. Syrjanen KJ. Condylomatous changes in neoplastic bronchial epithelium. Report of a case. Respiration 1979;38(5):299-304. Go to original source... Go to PubMed...
    8. Rubel L, Reynolds RE. Cytologic description of squamous cell papilloma of the respiratory tract. Acta Cytol 1979;23(3):227-31.
    9. Xiong WM, Xu QP, Li X, Xiao RD, Cai L, He F. The association between human papillomavirus infection and lung cancer: a system review and meta-analysis. Oncotarget 2017;8(56):96419-32. Go to original source... Go to PubMed...
    10. Zhai K, Ding J, Shi HZ. HPV and lung cancer risk: a meta-analysis. J Clin Virol 2015;63:84-90. Go to original source... Go to PubMed...
    11. Syrjanen K. Detection of human papillomavirus in lung cancer: systematic review and meta-analysis. Anticancer Res 2012;32(8):3235-50.
    12. Ragin C, Obikoya-Malomo M, Kim S, Chen Z, Flores-Obando R, Gibbs D, Koriyama C, Aguayo F, Koshiol J, Caporaso NE, Carpagnano GE, Ciotti M, Dosaka-Akita H, Fukayama M, Goto A, Spandidos DA, Gorgoulis V, Heideman DA, van Boerdonk RA, Hiroshima K, Iwakawa R, Kastrinakis NG, Kinoshita I, Akiba S, Landi MT, Eugene LH, Wang JL, Mehra R, Khuri FR, Lim WT, Owonikoko TK, Ramalingam S, Sarchianaki E, Syrjanen K, Tsao MS, Sykes J, Hee SW, Yokota J, Zaravinos A, Taioli E. HPV-associated lung cancers: an international pooled analysis. Carcinogenesis 2014;35(6):1267-75. Go to original source... Go to PubMed...
    13. Srinivasan M, Taioli E, Ragin CC. Human papillomavirus type 16 and 18 in primary lung cancers--a meta-analysis. Carcinogenesis 2009;30(10):1722-8. Go to original source... Go to PubMed...
    14. Anantharaman D, Gheit T, Waterboer T, Halec G, Carreira C, Abedi-Ardekani B, McKay-Chopin S, Zaridze D, Mukeria A, Szeszenia-Dabrowska N, Lissowska J, Mates D, Janout V, Foretova L, Bencko V, Rudnai P, Fabianova E, Tjonneland A, Travis RC, Boeing H, Quiros JR, Johansson M, Krogh V, Bueno-de-Mesquita HB, Kotanidou A, Clavel-Chapelon F, Weiderpass E, Johansson M, Pawlita M, Scelo G, Tommasino M, Brennan P. No causal association identified for human papillomavirus infections in lung cancer. Cancer Res 2014;74(13):3525-34. Go to original source... Go to PubMed...
    15. GmbH RD. Cobas®4800 HPV Test. Roche Molecular Systems Inc 2012
    16. GmbH. PapilloCheck® high-risk. Greiner Bio-One 2012
    17. Koshiol J, Rotunno M, Gillison ML, Van Doorn LJ, Chaturvedi AK, Tarantini L, Song H, Quint WG, Struijk L, Goldstein AM, Hildesheim A, Taylor PR, Wacholder S, Bertazzi PA, Landi MT, Caporaso NE. Assessment of human papillomavirus in lung tumor tissue. J Natl Cancer Inst 2011;103(6):501-7. Go to original source... Go to PubMed...
    18. Coissard CJ, Besson G, Polette MC, Monteau M, Birembaut PL, Clavel CE. Prevalence of human papillomaviruses in lung carcinomas: a study of 218 cases. Mod Pathol 2005;18(12):1606-9. Go to original source... Go to PubMed...
    19. Galvan A, Noci S, Taverna F, Lombardo C, Franceschi S, Pastorino U, Dragani TA. Testing of human papillomavirus in lung cancer and non-tumor lung tissue. BMC Cancer 2012;12:512. Go to original source... Go to PubMed...
    20. van Boerdonk RA, Daniels JM, Bloemena E, Krijgsman O, Steenbergen RD, Brakenhoff RH, Grunberg K, Ylstra B, Meijer CJ, Smit EF, Snijders PJ, Heideman DA. High-risk human papillomavirus-positive lung cancer: molecular evidence for a pattern of pulmonary metastasis. J Thorac Oncol 2013;8(6):711-8. Go to original source... Go to PubMed...
    21. Cheng YW, Chiou HL, Sheu GT, Hsieh LL, Chen JT, Chen CY, Su JM, Lee H. The association of human papillomavirus 16/18 infection with lung cancer among nonsmoking Taiwanese women. Cancer Res 2001;61(7):2799-803.
    22. Yu Y, Liu X, Yang Y, Zhao X, Xue J, Zhang W, Yang A. Effect of FHIT loss and p53 mutation on HPV-infected lung carcinoma development. Oncol Lett 2015;10(1):392-8. Go to original source... Go to PubMed...
    23. Xu Y, Cheng B, Pan H, Wu A, Zhang L. [The Relationship between the Status of Human Papillomavirus 16/18 Infection and the Expression of Bcl-2 and Bax in Squamous Cell Carcinomas of the Lung.]. Zhongguo Fei Ai Za Zhi 2009;12(8):849-52. Go to PubMed...
    24. Xiong WM, He F, Xiao RD, Yu TT, Zhang X, Liu ZQ, Xu QP, Cai L. [Association between human papillomavirus infection and lung cancer]. Zhonghua Liu Xing Bing Xue Za Zhi 2016;37(12):1658-61. Go to PubMed...
    25. Argyri E, Tsimplaki E, Marketos C, Politis G, Panotopoulou E. Investigating the role of human papillomavirus in lung cancer. Papillomavirus Res 2017;3:7-10. Go to original source... Go to PubMed...
    26. de Freitas AC, Gurgel AP, de Lima EG, de Franca Sao MB, do Amaral CM. Human papillomavirus and lung cancinogenesis: an overview. J Cancer Res Clin Oncol 2016;142(12):2415-27. Go to original source... Go to PubMed...
    27. Baba M, Castillo A, Koriyama C, Yanagi M, Matsumoto H, Natsugoe S, Shuyama KY, Khan N, Higashi M, Itoh T, Eizuru Y, Aikou T, Akiba S. Human papillomavirus is frequently detected in gefitinib-responsive lung adenocarcinomas. Oncol Rep 2010;23(4):1085-92. Go to original source... Go to PubMed...
    28. Peitsaro P, Johansson B, Syrjanen S. Integrated human papillomavirus type 16 is frequently found in cervical cancer precursors as demonstrated by a novel quantitative real-time PCR technique. J Clin Microbiol 2002;40(3):886-91. Go to original source... Go to PubMed...

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