Biomedical papers - Latest articles
Results 121 to 127 of 127:
Global DNA methylation and increased DNMT3A expression in multiple myeloma patientsOriginal papers
Petra Luzna, Denisa Weiser Drozdkova, Pavla Flodrova, Katarina Ondruskova, Ivo Uberall, Jiri Minarik, Zdenek Kolar, Katerina Smesny Trtkova
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(1):43-49 | DOI: 10.5507/bp.2022.006 
Multiple myeloma (MM) is a clonal malignancy characterized by proliferation of plasma cells in bone marrow. Epigenetic anomalies have been identified as important drivers in MM development. This study describes the level of methylation and/or expression changes of selected DNA methyltransferases in both unsorted and sorted bone marrow aspirates of multiple myeloma patients.
Detection of galanin receptors in the spinal cord in experimental autoimmune encephalomyelitisOriginal papers
Danica Michalickova, Ivana Kramarikova, Hatice Kubra Ozturk, Tomas Kucera, Tomas Vacik, Tomas Hrncir, Nikolina Kutinova Canova, Martin Sima, Ondrej Slanar
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(1):36-42 | DOI: 10.5507/bp.2022.005
This study investigated the expression of galanin receptors (GalR1, GalR2, GalR3) in the spinal cord in a murine model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE) by qPCR analysis and determined GalR1 cellular localization (oligodendrocytes, microglia, astrocytes, ependymal cells, and endothelial cells in the capillaries) by immunohistochemistry. qPCR analysis revealed that GalR1 mRNA levels is decreased in the EAE mice compared to controls, whereas mRNA level of GalR2 and GalR3 were not significantly different from the controls. Immunochemical detection of the GalR1 revealed its expression in the ependymal and endothelial cells. This study provides new evidence of the involvement of galanin in EAE pathophysiology, but further research is warranted.
Cerebrospinal fluid neurofilament light chains and CXCL13 as predictive factors for clinical course of multiple sclerosisOriginal papers
Pavel Hradilek, Kamila Zondra Revendova, Jana Horakova, Radovan Bunganic, Ondrej Pelisek, David Zeman, Pavla Hanzlikova, Pavlina Kusnierova
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(1):30-35 | DOI: 10.5507/bp.2023.002
Cerebrospinal fluid neurofilament light chain (cNfL) and CXCL13 concentrations were measured in 44 patients with clinically isolated syndrome or early multiple sclerosis. Neither cNfL nor CXCL13 did influence the risk of the second relapse or development of MRI activity. After adjustment for age, sex and treatment, higher cNfL levels were weakly associated with earlier worsening of neurological status.
Blood urea nitrogen - independent marker of mortality in sepsisOriginal papers
Martin Harazim, Kaiquan Tan, Marek Nalos, Martin Matejovic
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(1):24-29 | DOI: 10.5507/bp.2022.015
Predicting sepsis outcomes remains a challenge. In this retrospective study of two separate cohorts, we sought to determine the relationship between BUN levels on admission and 28-day mortality in sepsis. We provide new high-level information for intensivists to adopt blood urea nitrogen (BUN) as an easily available prognostic marker in sepsis.
Complete revascularization of multivessel coronary artery disease in patients with ST elevation acute coronary syndrome - for whom and when? A comprehensive reviewReviews
Miloslav Spacek, Jan Vacha, Jan Precek, Martin Hutyra, Radomir Nykl, Martin Sluka, Milos Taborsky
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(1):16-23 | DOI: 10.5507/bp.2022.024
A large proportion of patients with acute ST elevation myocardial infarction have significant residual stenoses exclusive of an infarct related artery. There is conflicting data regarding the optimal treatment strategy as well as its timing in such patients. In this article, we provide a comprehensive overview of this important and currently highly debated topic.
Floppy iris syndrome associated with specific medication intake: A narrative reviewReviews
Miroslava Maluskova, Ales Vidlar, Klara Maresova, Veronika Lounova, Marta Karhanova
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(1):9-15 | DOI: 10.5507/bp.2022.042
Intraoperative floppy iris syndrome (IFIS) is a cataract surgery complication that remains a challenge for eye surgeons. IFIS is most commonly associated with chronic use of alpha-1 adrenergic receptor antagonists in patients with lower urinary tract symptoms; however, it has been reported in other drug groups as well. This article is intended especially for urologists, ophthalmologists and internal medicine specialists, to review all the medications that can be associated with development of IFIS.
From a bispecific monoclonal antibody to gene therapy: A new era in the treatment of hemophilia AReviews
Romeo Gabriel Mihaila
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(1):1-8 | DOI: 10.5507/bp.2022.046
Emicizumab, a bispecific-humanized monoclonal antibody, is able to improve coagulation by bridging activated factor IX and factor X. It is administered subcutaneously and much less often compared to factor VIII products, has low immunogenicity, does not require dose adjustment, and can be administered regardless of the presence of factor VIII inhibitors. Gene therapy can elevate factor VIII plasma levels for many years after a single treatment course, could offer long-term protection from bleeding episodes, and minimize or eliminate the need for substitutive treatment with factor VIII concentrates.