Biomedical papers - Latest articles

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Prediction of live birth - selection of embryos using morphokinetic parametersOriginal papers

Katerina Tvrdonova, Silvie Belaskova, Tatana Rumpikova, David Rumpik, Alena Myslivcova Fucikova, Frantisek Malir

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2024, 168(1):74-80 | DOI: 10.5507/bp.2022.052  

The goal of this study was to use time-lapse monitoring to find key morphokinetic parameters in pre-implantation human embryos, which are essential for the identification of embryos with high developmental potential, specifically embryos leading to the birth of a healthy child.

DNA methylation of selected tumor suppressor genes in endometrial hyperplasiaOriginal papers

Ondrej Dvorak, Munachiso Ndukwe, Marcela Slavickova, Jan Laco, Jiri Spacek

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2024, 168(1):68-73 | DOI: 10.5507/bp.2022.053  

This research study was focused on changes in DNA methylation in specific tumor supressor genes in endometrial hyperplasia. The authors performed a retrospective study of endometrial tissue of 164 patients treated in their department from 2007 to 2014 dividing them into three subsets: atypical hyperplasia, hyperplasia without atypia and a control group with normal endometrial tissue. 25 selected genes using the methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) probe set were tested. Differences in DNA methylation among the groups were found in PTEN, CDH13, and MSH6 promoters. These genes appear to be involved in different stages of the development of endometrial hyperplasia.

The nociceptive response during adult cardiac surgery measured by the qNOX index: A feasibility studyOriginal papers

Vladislav Rogozov, Tomas Vanek, Marek Maly, Jan Rocek, Petr Kacer

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2024, 168(1):62-67 | DOI: 10.5507/bp.2022.045  

The "qNOX" index for the Conox monitor was predicted to be responsive to the probability of reaction to nociceptive stimulation during general anaesthesia. The results show that the qNOX index does not reflect development of detectable signs related to nociceptive stimulation during adult cardiac surgery and could be affected by simultaneous use of muscle relaxants.

Corneal stromal lenticule transplantation for the treatment of corneal ulcersOriginal papers

Yun Min Klimesova, Martina Nemcokova, Magdalena Netukova, Alina-Dana Baxant, Marcela Hlavackova, Jana Kacerovska, Pavel Studeny

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2024, 168(1):55-61 | DOI: 10.5507/bp.2023.004  

The possibility of using deep-frozen corneal stromal lenticules could significantly help in the treatment of corneal ulcers or even perforations, which is currently difficult due to the relative unavailability of corneal tissue donors, especially in developing countries.

Immunoablative therapy followed by autologous hematopoietic stem cell transplantation as the first-line disease-modifying therapy in patients with multiple sclerosisOriginal papers

Martin Lachnit, Kamila Zondra Revendova, Pavel Hradilek, Radovan Bunganic, Zdenek Koristek, Tomas Jelinek, Monika Skutova, Radim Piza, Ondrej Volny, Roman Hajek, Michal Bar

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2024, 168(1):50-54 | DOI: 10.5507/bp.2023.023  

Six multiple sclerosis (MS) patients with a rapid progression of disability underwent immunoablative therapy followed by autologous hematopoietic stem cell transplantation (AHSCT) as the first-line disease-modifying therapy, between 2018 and 2021. Four out of six patients showed disability progression after AHSCT, so the rapid progression of MS was just slowed down by AHSCT. One patient developed activity on magnetic resonance imaging and two patients experienced mild relapses after AHSCT. None of the patients developed grade 4 non-hematological toxicity.

Hypertension, hyperlipidaemia and thrombophilia as the most common risk factors for retinal vein occlusion in patients under 50 yearsOriginal papers

Zuzana Schreiberova, Jiri Rehak, Barbora Babkova, Martin Sin, Martina Rybarikova, Barbora Paskova, Irena Sinova, Petra Hubnerova, Miroslava Maluskova, Klara Maresova, Marta Karhanova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2024, 168(1):44-49 | DOI: 10.5507/bp.2022.036  

The authors confirmed that the most common abnormalities leading to retinal vein occlusion (RVO) in patients under 50 years were systemic cardiovascular diseases, particularly arterial hypertension and hyperlipidaemia, and we found out that the risk factors for central RVO (CRVO), hemi-central RVO (HRVO) and branch RVO (BRVO) were similar. To the best of our knowledge, this is the only study to date which has examined the difference between RF presence in CRVO and HRVO patients compared to BRVO patients under the age of 50.

Mortality of hospitalized patients with COVID-19: Effects of treatment options (vitamin D, anticoagulation, isoprinosine, ivermectin) assessed by propensity score matching, retrospective analysisOriginal papers

Jiri Plasek, Jozef Dodulik, Petr Gai, Barbora Hrstkova, Jan Skrha, Lukas Zlatohlavek, Renata Vlasakova, Peter Danko, Petr Ondracek, Eva Cubova, Bronislav Capek, Marie Kollarova, Tomas Furst, Jan Vaclavik

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2024, 168(1):35-43 | DOI: 10.5507/bp.2023.045  

The main findings of this retrospective analysis on 790 unvaccinated patients with COVID-19 respiratory infection may be summarized as follows: Vitamin D supplementation had no meaningful effect on in-hospital mortality. Neither isoprinosine nor ivermectin had any effect on in-hospital mortality either. Prophylactic anticoagulation treatment was to some degree beneficial compared with the full dose anticoagulation. 35.7% of patients died in the hospital: 37.7% males and 33.3% females, and the difference between sexes was not significant (except for the age group 61-70 years)

The inhibitory effects of Xiao-Gao-Jiang-Zhuo-containing serum on adipogenesis in 3T3-L1 preadipocytesOriginal papers

Liang Chen, Yu Han, Jing Li, Chunpeng Feng, Chen Chen, Ting Ye

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2024, 168(1):25-34 | DOI: 10.5507/bp.2023.016  

XGJZ-containing serum was obtained from obese rats treated with the XGJZ formula. The authors confirmed that XGJZ-containing serum can inhibit the differentiation of 3T3-L1 adipocytes, reduce the expression of lipogenic transcription factors, lipid accumulation, and lipid metabolism-related enzyme activity in differentiated 3T3-L1 adipocytes, thereby improving the risk of obesity and obesity-related diseases. It provides evidence for the mechanism of obesity

DNA damage and arterial hypertension. A systematic review and meta-analysisReviews

Radka Hazukova, Martina Rezacova, Miloslav Pleskot, Zdenek Zadak, Eva Cermakova, Milos Taborsky

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2024, 168(1):15-24 | DOI: 10.5507/bp.2023.044  

DNA damage is increased in hypertensive pts compared with healthy controls and this is confirmed with meta-analysis (comet: 26.6±11.0 vs 11.7±4.07; P<0.05; 8OHdG: 13.1±4.12 vs 6.97±2,67; P<0.05, gammaH2AX: no data available). Greater DNA damage is identified in more adverse cases. DNA damage correlates strongly with serum glycosylated haemoglobin (r=0.670; P<0.05) and total antioxidant status (r=-0.670 to -0.933; P<0.05). This is the first systematic review with meta-analysis showing that oxidative DNA damage is increased in humans with arterial hypertension.

Catheter ablation for non-paroxysmal atrial fibrillation. A reviewReviews

Sabri Hassouna, Pavel Osmancik

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2024, 168(1):1-14 | DOI: 10.5507/bp.2023.053  

Atrial fibrillation (AF) is the most common cardiac arrhythmia and it is associated with increased morbidity and mortality. Catheter ablation is currently the most effective treatment for AF. PVI as a treatment for paroxysmal atrial fibrillation (AF) is associated with a high success rate; however, outcomes for treating persistent and long-standing persistent AF with PVI alone are substantially lower. Since pulmonary vein isolation by itself is rarely sufficient in the treatment of non-paroxysmal AF, a variety of techniques have been developed to increase the efficacy of CA for non-paroxysmal AF. Different approaches include PVI-only, PVI + linear ablations, and a stepwise approach, depending on the surgeon's skills, preference, and experience. New methods such as pulsed-field ablation, using ultra-short electrical pulses, appear to be very promising.

Comprehensive home-based telerehabilitation in a morbidly obese male patient with severe obstructive sleep apnea. A case reportCase reports

Jakub Hnatiak, Lujza Zikmund Galkova, Petr Winnige, Ladislav Batalik, Filip Dosbaba, Ondrej Ludka, Jan Krejci

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):390-394 | DOI: 10.5507/bp.2023.022  

OSA is the most common of sleep-related breathing disorders in males. It adversely affects not only the patient's sleep quality, but their overall QoL as well. Even though CPAP therapy represents the standard treatment for moderate to severe OSA, rehabilitation should be considered as an additional therapy to further alleviate OSA symptoms. In this case report we show significant improvement of severe OSA in a male patient after the completion of the novel 12-week, comprehensive tele-RHB program. Our findings suggest that this tele-RHB program may be a novel home-based treatment to be used in conjunction with CPAP therapy.

Pulmonary damage in a patient with hairy cell leukemia - infectious involvement or hematological disease activity? Case reportCase reports

Dominika Ecsiova, Katerina Kamaradova, Marketa Nova, Petr Hoffmann, Petra Rozsivalova, Martin Simkovic, Pavel Zak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):385-389 | DOI: 10.5507/bp.2022.026  

This case report describes the management of treatment of HCL in a 53-year-old woman who developed a rare lung condition as part of the activity of the hematological disease. The authors present the differential diagnosis of pulmonary disease in patients with HCL and the treatment approach in these patients.

Hyperprogression on anti-PD-1 treatment. Is subsequent therapy feasible? A case report and review of the literatureCase reports

Jan Skacel, Bohuslav Melichar, Beatrice Mohelnikova-Duchonova, Radmila Lemstrova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):376-384 | DOI: 10.5507/bp.2022.025  

Hyperprogression is a new phenomenon that has emerged with the advent of immune checkpoint inhibitors. Prompt recognition and change of the treatment management is crucial. Chemotherapy based on taxanes is a reasonable option after hyperprogression. Hyperprogression is rarely observed with anti-CTLA-4 treatment.

Three-year follow-up results of two children born from a transplanted uterusOriginal papers

Jan Janota, Ekaterina Orlova, Marta Novackova, Roman Chmel Jr., Radim Brabec, Zlatko Pastor, Roman Chmel

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):370-375 | DOI: 10.5507/bp.2023.042  

Uterus transplantation appears to be a perspective treatment modality of absolute uterine factor infertility but it is still at an experimental stage worldwide. In this article, observational data on pregnancy outcomes, neonatal course, growth trajectory and 3-year follow-up in two children born at our institution in 2019 to mothers after uterus transplantation were analyzed, including assessment of neurodevelopment using the Bayley Scales of Infant and Toddler Development, third edition (Bayley-III). No abnormalities were observed in either prematurely born babies in terms of the neonatal course and 3-year follow-up, including the neurodevelopmental status. This is the first ever report on neurodevelopmental outcomes in children born after uterus transplantation.

Pars plana vitrectomy for vitreoretinal interface disorders coincident with intermediate stage age-related macular degenerationOriginal papers

Anna Tarkova, Libor Hejsek, Tomas Jurecka, Petra Wolkova, Martina Rubesova, Michal Hackl

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):366-369 | DOI: 10.5507/bp.2022.047  

Vitreoretinal surgery for vitreoretinal disorders has similar anatomical results whether or not the outer retina is damaged by intermediate AMD. Functional results are naturally affected by the eventual disruption of the retinal pigment epithelium or photoreceptors by an age-related process. The vitreoretinal surgery has a standard security profile. It is safe and does not affect the progression of AMD in the short term.

Thoracoscopic epicardial ablation of atrial fibrillation: Safety, efficacy, single center experienceOriginal papers

Martin Troubil, Martin Simek, Jan Juchelka, Andrea Steriovsky, Roman Hajek, Petr Santavy

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):362-365 | DOI: 10.5507/bp.2022.041  

An observational study evaluating the safety and efficacy of thoracoscopic ablation of atrial fibrillation in the single-center experience. The cohort consisted of 81 patients suffering from some form of atrial fibrillation, undergoing thoracoscopic ablation either as a single method of treatment or as a part of the hybrid protocol where catheter ablation was suplemented later.

Assessment of defecation disorders using high-resolution MRI-defecographyOriginal papers

Ioana Duca, Ramona Boja, Dan Lucian Dumitrascu

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):357-361 | DOI: 10.5507/bp.2022.023  

As clinical examination is inadequate in functional defecation disorders, and many multiparous women are at risk for outlet obstruction constipation, our diagnostic approach emphasizes the importance of correlating dynamic MRI-defecography, anorectal manometry and psychiatric disorders. Hypertonic anal sphincter and lack of defecation reflex at anorectal manometry correlated with anismus in all patients at MRI-defecography. Many cases with terminal constipation and anismus had a good correlation between defecation dysfunction and MRI-defecography pattern and it was also often associated with anxiety-depressive disorders.

The role of abdominal ultrasonography in patients with isoattenuating pancreatic carcinomaOriginal papers

Robert Psar, Ondrej Urban, Tomas Rohan, Michal Stepan, Martin Hill, Marie Cerna

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):352-356 | DOI: 10.5507/bp.2022.032  

Abdominal ultrasonography can detect secondary signs of isoattenuating pancreatic tumours with high sensitivity (88%) and has the potential to directly visualise these tumours in 21%. In this study, endoscopic ultrasonography detected a dilated pancreatic duct in isoattenuating pancreatic cancers significantly more often than abdominal ultrasonography.

Evaluation of aortic stiffness by echocardiography in tympanosclerosis patientsOriginal papers

Hikmet Hamur, Oruc Alper Onk, Resit Coskun, Muharrem Said Cosgun, Ismail Salcan, Izzet Emir

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):347-351 | DOI: 10.5507/bp.2022.031  

Aortic stiffness is a well-known predictor for developing atherosclerosis. Similar pathophysiological processes based on inflammation may explain aortic stiffness and tympanosclerosis formation. In this study, aortic stiffness parameters and blood inflammatory parameters were impaired in tympanosclerosis-detected patients.

Long-term outcomes of bipolar transurethral enucleation and resection of the prostate on patients with benign prostatic obstruction: a 10-year follow-upOriginal papers

Peijie Chen, Peng Xu, Chunxiao Liu

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):340-346 | DOI: 10.5507/bp.2022.034  

This 10-year follow-up indicates that B-TUERP is a safe and highly effective treatment for symptomatic BPH with lower reoperation rates. UDS value plays an important role in the preoperative judgment of surgical indications and the prediction of postoperative effect. Age, OABSS, PSA, MCC and DO may be critical predictors for urge incontinence after B-TUERP.

CCL2, CCL8, CXCL12 chemokines in resectable non-small cell lung cancer (NSCLC)Original papers

Marie Drosslerova, Martina Sterclova, Alice Taskova, Vladislav Hytych, Eva Richterova, Magdalena Bruzova, Miloslav Spunda, Martin Komarc, Martina Koziar Vasakova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):335-339 | DOI: 10.5507/bp.2022.050  

Lung cancer is the most common type of cancer in the world. It is unclear whether the concentrations of chemokines at the time of NSCLC (non-small cell lung cancer) diagnosis differ from healthy controls and reflect the extent of NSCLC. This study focuses on chemokine concentrations in the plasma of patients with resectable NSCLC vs healthy individuals and chemokine concentrations according to stage of NSCLC.

Overweight and obesity in children and adolescents with endocrine disordersReviews

Renata Pomahacova, Petra Paterova, Eva Nykodymova, Petr Polak, Eva Sladkova, Eva Skalicka, Josef Sykora

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):328-334 | DOI: 10.5507/bp.2023.036  

This article describes endocrine disorders which may play a role in the etiology of obesity in childhood. Though these are uncommon in overweight and obese children, late diagnosis may be associated with high morbidity or rarely, mortality. The authors describe the pathophysiological mechanisms responsible for the development of some forms of obesity in childhood and related clinical, diagnostic and management issues including factors such as growth patterns and BMI charts leading to the diagnosis of these conditions. Timely diagnosis of endocrinopathy and prompt treatment are necessary.

Current status and future perspectives of oral HPV testing in the diagnosis and monitoring of oropharyngeal cancer. A reviewReviews

Zuzana Horákova, Ivo Starek, Richard Salzman

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):319-327 | DOI: 10.5507/bp.2023.040  

HPV16 status in oropharyngeal cancer (OPC) is an important prognostic factor. As its determination, based on immunistochemical analysis of p16 oncoprotein requires invasive biopsy, determining oral HPV16 status may be a promising alternative. With an average sensitivity and specificity of 74% and 91% resp. oral sampling appears to be valuable in monitoring treatment response, enabling early detection of persistent or relapsing carcinoma sufficiently long before its clinical and/or radiological manifestation. It may also contribute to identification of the primary tumour in cases of metastases of unknown origin. The screening could identify individuals who are at increased risk of developing OPC.

The contribution of new methods in cytology for increasing sensitivity in the diagnosis of extrahepatic bile duct lesionsReviews

Daniela Kurfurstova, Zuzana Slobodova, Vincent Zoundjiekpon, Ondrej Urban

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(4):309-318 | DOI: 10.5507/bp.2023.041  

This is a review of current and new methods for improving on the sensitivity of biliary stenoses examinations, especially in relation to suspected carcinoma. Included are fluorescence in situ hybridization, next generation sequencing, the immunocolorimetric ELISA test, methylation markers of DNA and liquid. biopsy.

Chronic lymphocytic inflammation with pontine perivascular enhancement responsive to steroids (CLIPPERS) syndrome: Treatment approach depends on disease courseCase reports

Slavomira Kovacova, Petra Hnilicova, Jan Grossmann, Gabriel Hajas, Ema Kantorova, Egon Kurca

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(3):303-308 | DOI: 10.5507/bp.2022.013  

This paper describes two cases of CLIPPERS. The first demonstrates the benefit of the early introduction of corticosteroids with side effects from long-term treatment. The second demonstrates the efficacy of ocrelizumab (anti-CD20 molecule) in a severe course of CLIPPERS. To the author s knowledge, this is only the second published case of CLIPPERS treated with ocrelizumab. The aim was to draw attention to this rare, often misdiagnosed but treatable disease.

Acute drug intoxication in childhood: a 10-year retrospective observational single-centre study and case reportsCase reports

Petra Matalova, Michal Buchta, Vendula Drietomska, Alena Spicakova, Martin Wawruch, Peter Ondra, Karel Urbanek

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(3):294-302 | DOI: 10.5507/bp.2023.032  

Medication poisoning in children is one of the severe conditions that can endanger a child's life. We examined the demographic and clinical data of children admitted to the Department of Pediatrics of the University Hospital Olomouc for acute drug-induced intoxication between January 1, 2010, and December 31, 2019. From the results and determination of the two most common age groups, we need to understand drugs from several perspectives. To young children, medicines, especially colored ones, are perceived as attractive-looking. For older children and adolescents, medication can be an unfortunate way out of their difficulties. Medicines should be kept out of the reach of children and parents should educate their children.

Ethambutol-induced optic neuropathyCase reports

Martin Kyncl, Martin Fus, Jan Lestak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(3):288-293 | DOI: 10.5507/bp.2022.022  

This case report describes a tuberculosis patient treated with ethambutol for 3 months, which resulted in ethambutol-induced optic neuropathy (EON). The use of electrophysiological examinations, visual field test, retinal nerve fiber layer and angiography of optic nerve head, and magnetic resonance imaging of the optic nerves confirmed the improvement of visual function after discontinuation of the medication, irreversible atrophy of the optical disc has still been confirmed.

Trends in management of ocular syphilis in tertiary uveitis centre in Prague, Czech RepublicOriginal papers

Aneta Klimova, Michaela Brichova, Jarmila Heissigerova, Eva Rihova, Petra Svozilkova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(3):281-287 | DOI: 10.5507/bp.2022.038  

Visual field defects persist after antibiotic treatment in one third of patients with syphilitic uveitis. The observation supports adjacent systemic corticosteroid treatment for severe forms of syphilitic uveitis and/or neuritis.

Real-world outcomes of mepolizumab treatment in severe eosinophilic asthma patients - retrospective cohort study in SlovakiaOriginal papers

Milos Jesenak, Vaclav Vanecek, Martina Ondrusova, Veronika Urdova, Katarina Dostalova, Ludek Hochmuth

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(3):272-280 | DOI: 10.5507/bp.2023.029  

Mepolizumab treatment has shown improved asthma control and lung function in randomized controlled trials. Real-world data are important to validate these results, may significantly expand the clinical view of the use of mepolizumab and highlight some aspects that cannot be drawn from clinical trials. Data on mepolizumab from the Slovak clinical environment were requested by health insurance payers for successful patient access to the drug, but such data were never published. The presented data from a non-interventional study provide the first clinical evidence in Slovakia of the efficacy of mepolizumab in a real sample of patients with severe asthma when it is administered in routine care.

Risk stratification using growth differentiation factor 15 in patients undergoing transcatheter aortic valve implantationOriginal papers

Martin Sluka, Martin Hutyra, Radomir Nykl, Jiri Ostransky, Tomas Furst, Pavla Petrova, Jan Precek, Stepan Hudec, Milos Taborsky

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2023, 167(3):263-271 | DOI: 10.5507/bp.2022.017  

Growth differentiation factor 15 (GDF15) is a cytokine that participates in inflammation and apoptosis. It has been identified as a strong and independent predictor of prognosis and adverse cardiac events in various cardiac conditions. Based on the data, this study supports a potential role of GDF15 in mortality and other clinical adverse outcomes such as bleeding complications and acute kidney injury prediction following transcatheter aortic valve implantation.