Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019, 163(2):132-140 | DOI: 10.5507/bp.2018.048

Serum C-peptide level correlates with the course of muscle tissue healing in the rabbit model of critical limb ischemia

Zdenek Taubera*, Katerina Cizkovaa,b*, Maria Janikovac,d, Jana Jurcikovae, Katerina Vitkovae, Lubomir Pavliskae, Ludmila Porubovaf, Agata Krauzeg, Carlos Fernandezg, Frantisek Jaluvkah, Iveta Spackovai, Ivo Lochmanj, Martin Prochazkac, Brian H. Johnstonek, Vaclav Prochazkal
a Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
b Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
c Department of Medical Genetics, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
d Department of Clinical and Molecular Pathology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Czech Republic
e Department of Deputy Director of Science and Research, University Hospital Ostrava, Czech Republic
f 4MEDi-CBTD, Ostrava, Czech Republic
g Center for Cardiovascular Research and Development, American Heart of Poland, Inc., Kostkowice, Poland
h Department of Surgery, University Hospital Ostrava, Czech Republic
i Laboratore AGEL, a.s., Novy Jicin, Czech Republic
j SPADIA LAB a.s., Ostrava, Czech Republic
k NeuroFX, Inc, Indianapolis, Indiana, USA
l Radiodiagnostic Institute, University Hospital Ostrava, Czech Republic

Aim: The therapeutic potential of adipose-derived stem cell conditioned medium (ASC-CM) was studied in the rabbit model of critical limb ischemia (CLI).

Methods: Rabbits received treatment with ASC-CM or placebo. Gastrocnemius muscle tissue was collected 35 days after ischemia induction. Ischemic changes were evaluated in hematoxylin-eosin stained tissues for early (necrotic lesions/granulation tissue) and late (fibrous scars) phases of tissue repair. The expression of proangiogenic miR-126 was also evaluated using in situ hybridization. The levels of cytokines, insulin, and C-peptide were measured in blood.

Results: Early repair phases were observed more often in placebo-treated samples (45.5%) than in ASC-CM-treated ones (22.2%). However, the difference was not statistically significant. We demonstrated a statistically significant positive correlation between the early healing phases in tissue samples and C-peptide levels in peripheral blood. The expression of proangiogenic miR-126 was also shown in a number of structures in all phases of ischemic tissue healing.

Conclusion: Based on our results, we believe that treatment with ASC-CM has the potential to accelerate the healing process in ischemic tissues in the rabbit model of CLI. The whole healing process was accompanied by miR-126 tissue expression. C-peptide could be used to monitor the course of the tissue healing process.

Keywords: mesenchymal stem cells, tissue healing, cytokines, C-peptide, miR-126, critical limb ischemia

Received: March 11, 2018; Accepted: August 17, 2018; Prepublished online: September 7, 2018; Published: June 25, 2019  Show citation

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Tauber, Z., Cizkova, K., Janikova, M., Jurcikova, J., Vitkova, K., Pavliska, L., ... Prochazka, V. (2019). Serum C-peptide level correlates with the course of muscle tissue healing in the rabbit model of critical limb ischemia. Biomedical papers, 163(2), 132-140. doi: 10.5507/bp.2018.048
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