Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2013, 157(3):233-238 | DOI: 10.5507/bp.2012.061

Occult tumour cells in peritoneal lavage are a negative prognostic factor in pancreatic cancer

Roman Havlika, Josef Srovnalb, Dusan Klosa, Andrea Benedikovab, Martin Loveceka, Mohamed Ghothima, Dana Cahovab, Cestmir Neorala, Marian Hajduchb
a Department of Surgery I, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
b Laboratory of Experimental Medicine, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc

Aims: The aim of this study was to test the hypothesis that occult tumour cells in peritoneal lavage are a negative prognostic factor in pancreatic adenocarcinoma.

Methods: Real-time RT-PCR analysis of CEA, EGFR and hTERT transcript levels was used to identify occult tumour cells in peritoneal lavage samples from 96 pancreatic cancer patients.

Results: We found significant association between CEA expression levels in peritoneal lavage and clinical stage. We also found that EGFR transcript levels were higher in peritoneal lavage samples from patients with high grade tumours than in samples from patients with low grade tumours. Detection of CEA and/or EGFR occult tumour cell markers in the peritoneal lavage was associated with significantly shorter overall survival and increased hazard ratio for disease recurrence.

Conclusions: The results show that the presence of occult tumour cells in peritoneal lavage is a negative prognostic factor for survival in pancreatic cancer patients, and that detection of occult tumour cells using PCR-based methods can identify patients with advanced disease for whom radical surgery is likely to have little benefit.

Keywords: pancreatic adenocarcinoma, occult tumour cells, peritoneal lavage, prognostic factor, RT-PCR

Received: February 2, 2012; Prepublished online: October 31, 2012; Published: September 30, 2013  Show citation

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Havlik, R., Srovnal, J., Klos, D., Benedikova, A., Lovecek, M., Ghothim, M., ... Hajduch, M. (2013). Occult tumour cells in peritoneal lavage are a negative prognostic factor in pancreatic cancer. Biomedical papers, 157(3), 233-238. doi: 10.5507/bp.2012.061
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