Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2011, 155(3):299-303 | DOI: 10.5507/bp.2011.043
PRECLINICAL ALTERATIONS OF ORAL EPITHELIAL CELLS IN CONTACT WITH ORTHODONTIC APPLIANCES
- a Department of Stomatology, School of Dentistry, Pontificia Universidade Catolica do Parana-PUCPR, Curitiba-PR, Brazil
- b Department of Stomatology, School of Dentistry, Universidade Federal do Parana-UFPR, Curitiba-PR, Brazil
Aim: This study evaluated the behavior of oral epithelial cells in contact with orthodontic appliances.
Methods: Oral epithelial cells of clinically normal buccal mucosa were obtained by liquid-based exfoliative cytology from 22 orthodontic patients. The following regions were evaluated: a) oral mucosa exposed to friction from orthodontic brackets; b) oral mucosa exposed to friction from the tube on the orthodontic band, and c) oral mucosa not exposed to friction (control area). Nuclear (NA) and cytoplasmic (CA) areas and NA/CA ratio were assessed by an image analysis system. Cell morphology and cellularity were also analyzed by Papanicolaou technique.
Results: The NA of the cells in contact with orthodontic brackets and bands were smaller than the control area. Cells in contact with the brackets showed the greatest reduction in CA in comparison with the cells subjected to friction from the tube, and the control group. Smears classified as type I predominated in all regions analyzed, although type II were predominant, together with superficial epithelial cells, mainly in the oral mucosa in contact with the band tube.
Conclusion: Preclinical alterations in the epithelial cells of oral mucosa, caused by orthodontic appliances, are reduction in NA, increase in cell keratinization and inflammatory features, especially in the band tube area.
Keywords: Oral epithelium, Exfoliative cytology, Brackets, Orthodontic band, Orthodontic appliance
Received: February 3, 2011; Accepted: July 14, 2011; Published: September 1, 2011 Show citation
ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- Nanci A. Ten Cates oral histology: Development, structure, and function. 7th ed. Mosby, 2007.
- Wolff A, Ship JA, Tylenda CA, Fox PC, Baum BJ. Oral mucosal appearance is unchanged in healthy, different-aged persons. Oral Surg Oral Med Oral Pathol 1991;71:569-72.
Go to original source...
Go to PubMed...
- Sognnaes RF, Albright JT. Electron microscopic study of the epithelial lining of the human oral mucosa. Oral Surg Oral Med Oral Pathol 1958;11:62-73.
Go to original source...
Go to PubMed...
- Schulman JD, Beach MM, Rivera-Hidalgo F. The prevalence of oral mucosal lesions in U.S. adults: data from the Third National Health and Nutrition Examination Survey, 1988-1994. J Am Dent Assoc 2004;135(9):1279-86.
Go to original source...
Go to PubMed...
- Schulman JD. Prevalence of oral mucosal lesions in children and youths in the USA. Int J Paediatr Dent 2005;15(2):89-97.
Go to original source...
Go to PubMed...
- Cotran RS, Kumar V, Collins T. Robbins pathologic basis of disease. Philadelphia: W.B. Saunders Company, 1999.
- Kluemper GT, Hiser DG, Rayens MK, Jay MJ. Efficacy of a wax containing benzo caine in the relief of oral mucosal pain caused by orthodontic appliances. Am J Orthod Dentofacial Orthop 2002;122(4):359-65.
Go to original source...
Go to PubMed...
- Pereira BR, Tanaka OM, Lima AA, Guariza-Filho O, Maruo H, Camargo ES. Metal and ceramic bracket effects on human buccal mucosa epithelial cells. Angle Orthod. 2009;79(2):373-9.
Go to original source...
Go to PubMed...
- Cowpe JG, Longmore RB, Green MW. Quantitative exfoliative cytology of normal oral squames: an age, site and sex-related survey. J R Soc Med 1985;78(12):995-1004.
Go to original source...
Go to PubMed...
- Ogden GR, Cowpe JG, Green MW. Quantitative exfoliative cytology of normal buccal mucosa: effect of smoking. J Oral Pathol Med 1990;19(2):53-5.
Go to original source...
Go to PubMed...
- Ogden GR, Cowpe JG, Wight AJ. Oral exfoliative cytology: review of methods of assessment. J Oral Pathol Med 1997;26(5):201-5.
Go to original source...
Go to PubMed...
- Ramaesh T, Mendis BRRN, Ratnatunga N, Thattil RO. Cytomorphometric analysis of squames obtained from normal oral mucosa and lesions of oral leukoplakia and squamous cell carcinoma. J Oral Pathol Med 1998;27(2):83-6.
Go to original source...
Go to PubMed...
- Diniz-Freitas M, Garcia-Garcia A, Crespo-Abelleira A, Martins- Carneiro JL, Gandara-Rey JM. Applications of exfoliative cytology in the diagnosis of oral cancer. Med Oral 2004;9(4):355-61.
Go to PubMed...
- Mehrotra R, Gupta A, Singh M, Ibrahim R. Application of cytology and molecular biology in diagnosing premalignant or malignant oral lesions. Mol Cancer 2006;5(11):5-11.
Go to original source...
Go to PubMed...
- Kwon OS, Chung JH, Cho KH, Suh DH, Park KC, Kim KH, Eun He. Nicotine-enhanced epithelial differentiation in reconstructed human oral mucosa in vitro. Skin Pharmacol Appl Skin Physiol 1999;12(4):227-34.
Go to original source...
Go to PubMed...
- Silverman SJR, Becks H, Farber SM. The diagnostic value of intraoral cytology. J Dent Res 1958;37(2):195-205.
Go to original source...
Go to PubMed...
- Zimmermann ER, Zimmermann AL. Effects of race, age, smoking habits, oral and systemic disease on oral exfoliative cytology. J Dent Res 1965;44(4):627-31.
Go to original source...
Go to PubMed...
- Ozcelik O, Haytac MC, Akkaya M. Iatrogenic trauma to oral tissues. J Periodontol 2005;76(10):1793-7.
Go to original source...
Go to PubMed...
- Regezi JA, Sciubba JJ. Oral pathology: clinical pathologic correlations. 3ed. Philadelphia: W. B. Saunders Company, 1999.
- Ogden GR, Wight AJ, Cowpe JG. Quantitative oral exfoliative cytology. Effect of alcohol on normal buccal mucosa. Anal Quant Cytol Histol 1999;21(2):126-30.
- Ogden GR, Wight AJ, Rice P. Effect of alcohol on the oral mucosa assessed by quantitative cytomorphometry. J Oral Pathol Med 1999;28(5):216-20.
Go to original source...
Go to PubMed...
- Ramaesh T, Mendis BR, Ratnatunga N, Thattil RO. The effect of tobacco smoking and of betel chewing with tobacco on the buccal mucosa: a cytomorphometric analysis. J Oral Pathol Med 1999;28(9):385-8.
Go to original source...
Go to PubMed...
- Alberti S, Spadella CT, Francischone TR, Assis GF, Cestari TM, Taveira LA. Exfoliative cytology of the oral mucosa in type II diabetic patients: morphology and cytomorphometry. J Oral Pathol Med 2003;32: 538-43.
Go to original source...
Go to PubMed...
- Einstein TB, Sivapathasundharam B. Cytomorphometric analysis of the buccal mucosa of tobacco users. Indian J Dent Res 2005;16(2):42-6.
- Pavanello MB, Prado FB, Balducci I, Brandao AA. Cytologic analysis of alterations induced by smoking and by alcohol consumption. Acta Cytol 2006;50(4):435-40.
Go to original source...
Go to PubMed...
- Jones AC, Migliorati CA, Stewart CM. Oral cytology: indications, contraindications, and technique. Gen Dent 1995;43(1):74-7.
Go to original source...
- Alves VAF, Castelo A, Filho AL, Vianna MR, Taromaru E, Namiyama G, Lorincz A, Dores GBD. Performance of the DNACitoliq liquid-based cytology system compared with conventional smears. Cytopathology 2006,17:86-93.
Go to original source...
Go to PubMed...
- Wever DJ, Veldhuizen AG, Sanders MM, Schakenraad JM, van Horn JR. Cytotoxic, allergic and genotoxic activity of a nickeltitanium alloy. Biomaterials. 1997;18(16):1115-20.
Go to original source...
Go to PubMed...
- Montanaro L, Cervellati M, Campoccia D, Prati C, Breschi L, Arciola CR. No genotoxicity of a new nickel-free stainless steel. Int J Artif Organs 2005;28(1):58-65.
Go to original source...
- Angelieri F, Carlin V, Martins RA, Ribeiro DA. Biomonitoring of mutagenicity and cytotoxicity in patients undergoing fixed orthodontic therapy. Am J Orthod Dentofacial Orthop. 2011;139(4 Suppl):e399-404.
Go to original source...
Go to PubMed...