Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2005, 149(2):381-384 | DOI: 10.5507/bp.2005.063

LUNG CYTOTOXICITY OF COMBINED EXPOSURE TO REFRACTORY CERAMIC FIBRES AND CIGARETTE SMOKE

Silvia Černá, Marta Hurbánková, Zuzana Kováčiková, Milan Beňo, Soňa Wimmerová
Slovak Medical University, Bratislava, Slovak Republic

Changes in some lung cytotoxic parameters after exposure to refractory ceramic fibres (RCF) or to cigarette smoke (S) and after combined exposure to RCF+S were studied in male Wistar rats in order to evaluate their potential adverse health effects. Four groups of rats were treated as follows : 1) intratracheally instilled by saline solution (0.4 ml); 2) intratracheally instilled by 4 mg of RCF; 3) exposed only to S (85 mg of total particulate matter/m3 air ) for two hours daily; 4) exposed to RCF+S. After 6 months the animals were exsanguinated and the bronchoalveolar lavage (BAL) was perfomed. Viability and phagocytic activity of alveolar macrophages (AM), activity of lactate dehydrogenase (LDH) in cell-free BAL fluid (cf-BALF), acid phosphatase (ACP) and cathepsin D (CATD) in cfBALF, in BALF cells and in the lung tissue were estimated. Viability of AM was depressed by every type of exposure with RCF+S effect being at least additive. Phagocytic activity of AM increased in the presence of RCF. No significant changes in LDH activity were found. Activities of lysosomal enzymes measured in the lung tissue homogenates were not significantly changed, but those in the cfBALF increased especially after exposure to S with most expressive increase in BALF cells after exposure to S and RCF+S. In the case of CATD the effect of RCF+S was more than additive. The results point out to the persistence of the RCF exposure cytotoxic effects and their amplification by cigarette smoke.

Keywords: Lungs, Bronchoalveolar lavage, Refractory ceramic fibres, Smoking, Cytotoxicity, Lysosomal enzymes

Received: June 10, 2005; Accepted: September 25, 2005; Published: December 1, 2005  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Černá, S., Hurbánková, M., Kováčiková, Z., Beňo, M., & Wimmerová, S. (2005). LUNG CYTOTOXICITY OF COMBINED EXPOSURE TO REFRACTORY CERAMIC FIBRES AND CIGARETTE SMOKE. Biomedical papers149(2), 381-384. doi: 10.5507/bp.2005.063
Download citation

References

  1. Foa V, Basilico S. (1999) Chemical and physical characteristics and toxicology of man made mineral fibres. Med Lav 90, 10-52. Go to PubMed...
  2. Maxim LD, Allshouse JN, Chen SH, Treadway JC, Venturin DE. (2000) Workplace Monitoring of Refractory Ceramic Fiber in the United States. Regul Toxicol Pharmacol 32, 293-309. Go to original source... Go to PubMed...
  3. Venturin D, Deren G, Corn M. ( 1997) Qualitative Industrial Hygiene Product Life Cycle Analysis Apllied to Refractory Ceramic Fiber (RCF) Consumer Product Applications. Regul Toxicol Pharmacol 25, 232-9. Go to original source... Go to PubMed...
  4. Searl A, Buchanan D, Cullen RT, Jones AD, Miller BG, Soutar CA. (1999) Biopersistence and durability of nine mineral fiber types in rat lungs over 12 months. Ann Occup Hyg 43, 143-53. Go to original source... Go to PubMed...
  5. Mühle H, Bellman B. (1995) Biopersistence of man-made vitreous fibres. Ann Occup Hyg 39, 655-60. Go to original source... Go to PubMed...
  6. Yamato H, Hori H, Tanaka I, Higashi T, Morimoto Y, Kido M. (1994) Retention and clearance of inhaled ceramic fibres in rat lungs and development of dissolution model. Occup Environ Med 51, 275-80. Go to original source... Go to PubMed...
  7. Dufrense A, Perrault G, Yamato H, Masse S, Begin R. (1999) Clearance of man made mineral fibres from the lungs of sheep. Occup Environ Med 56, 684-90. Go to original source... Go to PubMed...
  8. Mast RW, Hesterberg TW, Glass LR, McConnel EE, Anderson R, Bernstein DM. (1994) Chronic inhalation and biopersistence of refractory ceramic fibres in rats and hamsters. Environ Health Perspect 102, suppl. 5, 207-9. Go to original source...
  9. Hesterberg TW, Muller WC, Thevenaz P, Anderson R. ( 995) Chronic inhalation studies of man-made vitreous fibres: chtracterization of fibres in the exposure aerosol and lungs. Ann Occup Hyg 39, 661-72. Go to PubMed...
  10. Bernstein DM, Thevenaz P, Fleissner H, Anderson R, Hesterberg TW, Mast R. (1995) Evaluation of oncogenic potential of manmade vitreous fibres: the inhalation model. Ann Occup Hyg 39, 661-72. Go to original source... Go to PubMed...
  11. Rossiter CE, Chase JR. (1995) Statistical analysis of results of carcinogenity studies of synthetic vitreous fibres at Research and Consulting Company, Geneva. Ann Occup Hyg 39, 759-69. Go to original source... Go to PubMed...
  12. International Agency for Research on Cancer (IARC). Man- Made Mineral Fibres. In: Man-Made Mineral Fibres and Radon. Lyon: IARC, 1988. p. 39-71.
  13. Cowie HA, Wild P, Beck J, Auburtin G, Piekarsky C, Massin N, Cherrie JW, Hurley JF, Miller BG, Groat CA. ( 2001) An epidemiological study of workers in European refractory ceramic fiber industry. Occup Environ Med 58, 800-10. Go to original source... Go to PubMed...
  14. Hurbánková M, Keiglová A. (1999) Compared effects of asbestos and wollastonite fibrous dusts on various biological parameters measured in bronchoalveolar lavage fluid. J Trace Microprobe Tech 17, 233-43.
  15. Navarová J, Nosáµová V. (1994) Effects of H2 receptor antagonists on indometacin induced lysosomal enzyme release from gastric mucosa. Meth Fin Exp Clin Pharmacol 16,119-24,
  16. Barrett AJ, Heath MF. Lysosomal enzymes. In: Dingle JT.editor. Lysosomes: Laboratory Handbook, 2th ed. Amsterdam: Elsevier, 1977. p. 19-147.
  17. Navarová J, Seemanová Z, Ulházy E, ©otníková R, Dubovický M, Muchová S, Horáková K. (2000) Biochemical variables of oxidative cell and tissue damage induced by phenytoin. Biologia 55, Suppl 8, S20-23.
  18. Černá S, Beňo M, Hurbánková M, Kováčiková Z, Bobek P, Kyrtopoulos SA. (2004) Evaluation of bronchoalveolar lavage fluid cytotoxic parameters after inhalation exposure to amosite and wollastonite fibrous dusts combined with cigarette smoke. Centr Eur J Publ Health 12, Suppl 2, S20-3.
  19. Drent M, Cobben NAM, Henderson RF, Wounters EFM, Dieijen- Visser van MP. (1996) Usefulness of lactate dehydrogenase and its isoenzymes as indicators of lung damage or inflammation. Eur Respir J 9, 2416-8. Go to original source... Go to PubMed...
  20. Sjörstrand M, Rylander R. (1987) Lysosomal enzyme activity and fibroblast stimulation of lavage from guinea pigs exposed to silica dust. Br J Exp Path 68, 309-18.
  21. Sjörstrand M, Rylander R. (1984) Lung cell reaction in guinea pigs exposed to tobacco smoke and silica dust or bacterial lipopolysaccharides. Toxicology 29, 271-80. Go to original source... Go to PubMed...
  22. Kim KA, Lee WK, Kim JK, Seo MS, Lim Y, Lee KH, Chae G, Lee SH, Chung Y.( 2001) Mechanism of refractory ceramic fiber- and rockwool-induced cytotoxicity in alveolar macrophages. Int Arch Occup Environ Health 74, 9-15. Go to original source... Go to PubMed...
  23. Jaurand MC, Magne L, Bignon J, Goni J. (1980) Effects of welldefined fibres on red blood cells and alveolar macrophages. IARC Sci Publ 30, 441-50.
  24. Schimmelpfeng J, Seidel A. (1991) Cytotoxic effects of quartz and chrysotile asbestos: in vitro interspecies comparison with alveolar macrophages. J Toxicol Environ Helath 33, 131-40. Go to original source... Go to PubMed...
  25. Fraunhofer Institute of Toxicology and Aerosol Research. Final Report on a Short-Term Inhalation Study with RCF1 in Rats. Study 02G97012. Hannover, 2001.
  26. Rylander R, Fogelmark B, Sjörstrand M. (1985) Free lung cell phagocytosis and lysosomal enzyme activity after inhalation of lipopolysaccharide in guinea pigs. Agents Action 16, 353-8. Go to original source... Go to PubMed...
  27. Atwood EM, Weich DJ, Oosthuizen JM. (1996) The influence of carbon particles on the concentration of acid phosphatase and lysosomal enzyme within alveolar macrophages during killing and degradation of Mycobacterium bovis. Tuber Lung Dis 77, 341-7. Go to original source... Go to PubMed...
  28. Sulciman SA. (1987) Petroleum hydocarbon toxicity in vitro: effects of n-alkanes, benzene and toluene on pulmonary alveolar macrophages and lysosomal enzymes of lungs. Arch Toxicol 59, 402-7, Go to original source... Go to PubMed...
  29. Higashiyama M, Doi O, Kodama K, Yokouchi H, Kasugai T, Ishiguro S. (1997) Influence of cathepsin D expression in lung adenocarcinoma on prognosis: possible importance of its expression in tumor cells and stromal cells, and its intracellular polarization in tumor cells. J Surg Oncol 65, 10-9. Go to original source... Go to PubMed...
  30. Hausmann M, Obermeier F, Schreiter K, Spott T, Falk W, Scholmerich J, Herfarth H, Saftig P, Rogler G. (2004) Cathepsin is up-regulated in inflammatory bowel disease macrophages. Clin Exp Immunol 135, 157-67. Go to original source...
  31. Chang JC, Yoo OH, Lesser M. (1989) Cathepsin D activity is increased in alveolar macrophages and bronchoalveolar lavage fluid of smokers. Am Rew Resp Dis 140, 958-60. Go to original source... Go to PubMed...