Biomed. Papers 148(2), 141-144 (2004) | DOI: 10.5507/bp.2004.025

N-FORMYL-MET-LEU-PHE-INDUCED OXIDATIVE BURST IN DMSO-DIFFERENTIATED HL-60 CELLS REQUIRES ACTIVE HSP90, BUT NOT INTACT MICROTUBULES

Jiří Vrba, Martin Modrianský

Institute of Medical Chemistry and Biochemistry, Faculty of Medicine, Palacký University, Olomouc, Czech Republic

In this study we examined whether microtubules and heat shock protein 90 (Hsp90) are involved in phorbol myristate acetate (PMA) and N-formyl-Met-Leu-Phe (fMLP)-induced oxidative burst in DMSO-differentiated HL-60 cells. Our results showed that microtubule interfering agents, paclitaxel (1-5 µM), colchicine (1-100 µM), nocodazole (1-20 µM), and vincristine (1-50 µM), did not affect either PMA or fMLP-induced oxidative burst. In contrast, radicicol, an inhibitor of Hsp90, inhibited fMLP-induced oxidative burst in time and concentration-dependent manner where IC50 value for 30 min pre-incubation was 16.5 ± 3.5 µM radicicol. We conclude that both PMA and fMLP-induced oxidative burst in DMSO-differentiated HL-60 cells is microtubule-independent while the latter requires Hsp90 activity.

Keywords: Radicicol, Hsp90, Microtubules, Oxidative burst, HL-60

Received: September 9, 2004; Accepted: October 16, 2004; Published: December 1, 2004  Show citation