Biomed. Papers 147(2), 147-153 (2003) | DOI: 10.5507/bp.2003.020

Metal-chelating properties, electrochemical behavior, scavenging and cytoprotective activities of six natural phenolics

Jitka Psotová^a, Jan Lasovský^b, Jaroslav Vičar^a

^a Institute of Medical Chemistry and Biochemistry, Faculty of Medicine, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic

^b Department of Physical Chemistry, Faculty of Science, Palacký University, Tř. Svobody 26, 771 46 Olomouc, Czech Republic

Chelation, electrochemical, antioxidant and cytoprotective properties of six phenolics - cynarin and caffeic, chlorogenic, ferulic, protocatechuic and rosmarinic acids were studied on the following models: (i) chelation of transition metals, (ii) quenching of the diphenylpicrylhydrazyl radical (DPPH), (iii) determination of half-wave potential, (iv) erythrocytres or mitochondrial membranes damaged by tert-butyl hydroperoxide (tBH) and (v) a primary culture of rat hepatocytes intoxicated by Cu(II) and Fe(III) or tBH. All phenolics suppressed cell membrane damage induced by transition metals or tBH. The protectivity correlated with their capacity to bind transition metals, to scavenge DPPH radical and with the value of half-wave potentials. In in vitro assays, the most promising was rosmarinic acid.

Keywords: Phenolic acids, Chelatation, DPPH, Cyclic voltammetry, Lipid peroxidation, Rat mitochondria, Rat erythrocytes, Rat hepatocytes

Received: October 28, 2003; Accepted: December 5, 2003; Published: December 1, 2003  Show citation