Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015, 159(3):400-406 | DOI: 10.5507/bp.2015.028

Blood levels of antioxidants during age-related macular degeneration treatment by rheohaemapheresis

Jana Aufartovac, Milan Blahab, Eva Kasalovac, Barbora Honegrovac, Barbora Cervinkovac, Lenka Kujovska Krcmovaa,c, Jiri Plisekc, Miriam Lanskab, Lubos Sobotkaa, Dagmar Solichovaa
a 3rd Department of Internal Medicine - Metabolic Care and Gerontology, Faculty of Medicine in Hradec Kralove, Charles University in Prague and University Hospital Hradec Kralove, Czech Republic
b 4th Department of Internal Medicine - Hematology, Faculty of Medicine in Hradec Kralove, Charles University in Prague and University Hospital Hradec Kralove
c Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, Hradec Kralove

Aims: Rheohaemapheresis treatment influences rheological markers and most likely improves metabolism in affected retinal areas, resulting not only in absorption of soft drusen but also reduction or complete disappearance of drusenoid retinal pigment epithelium detachments. However, the character of the treatment process has raised suspicion that there is a decrease not only in cholesterol but also in antioxidants, such as vitamin E and vitamin A.

Methods: Twenty-three patients with the progressive dry form of age-related macular degeneration were each treated with 8 procedures of rheohaemapheresis. We measured levels of vitamin E (α-tocopherol), the vitamin E/cholesterol ratio in serum and lipoproteins (VLDL, LDL, HDL). Vitamin E in erythrocyte membrane and serum vitamin A (retinol) were also measured. These parameters were determined before and after rheohaemapheresis. Erythrocyte superoxide dismutase, erythrocyte glutathione peroxidase and serum malondialdehyde were analysed as markers of antioxidant activity and lipid peroxidation, respectively.

Results: In serum, the VLDL and LDL fraction ratios of vitamin E/cholesterol increased significantly. Additionally, the HDL fraction ratio showed an increase but this was not statistically significant. The patients showed no clinical signs of vitamin E deficiency, and their serum concentrations of vitamin E did not differ from normal values. The results show that rheohaemapheresis in addition to causing a significant reduction in atherogenic LDL cholesterol, may have favourable additive anti-atherogenic effects due to a relative increase in the content of vitamin E in the lipoprotein fractions.

Keywords: rheohaemapheresis, age related macular degeneration, vitamin E, vitamin E/cholesterol ratio, antioxidants

Received: February 2, 2015; Accepted: May 15, 2015; Prepublished online: June 12, 2015; Published: September 30, 2015  Show citation

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Aufartova, J., Blaha, M., Kasalova, E., Honegrova, B., Cervinkova, B., Kujovska Krcmova, L., ... Solichova, D. (2015). Blood levels of antioxidants during age-related macular degeneration treatment by rheohaemapheresis. Biomedical papers, 159(3), 400-406. doi: 10.5507/bp.2015.028
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