RT Journal Article
SR Electronic
A1 Tauber, Zdenek
A1 Cizkova, Katerina
A1 Janikova, Maria
A1 Jurcikova, Jana
A1 Vitkova, Katerina
A1 Pavliska, Lubomir
A1 Porubova, Ludmila
A1 Krauze, Agata
A1 Fernandez, Carlos
A1 Jaluvka, Frantisek
A1 Spackova, Iveta
A1 Lochman, Ivo
A1 Prochazka, Martin
A1 Johnstone, Brian H.
A1 Prochazka, Vaclav
T1 Serum C-peptide level correlates with the course of muscle tissue healing in the rabbit model of critical limb ischemia
JF Biomedical papers
YR 2019
VO 163
IS 2
SP 132
OP 140
DO 10.5507/bp.2018.048
UL https://biomed.papers.upol.cz/artkey/bio-201902-0005.php
AB Aim: The therapeutic potential of adipose-derived stem cell conditioned medium (ASC-CM) was studied in the rabbit model of critical limb ischemia (CLI). Methods: Rabbits received treatment with ASC-CM or placebo. Gastrocnemius muscle tissue was collected 35 days after ischemia induction. Ischemic changes were evaluated in hematoxylin-eosin stained tissues for early (necrotic lesions/granulation tissue) and late (fibrous scars) phases of tissue repair. The expression of proangiogenic miR-126 was also evaluated using in situ hybridization. The levels of cytokines, insulin, and C-peptide were measured in blood. Results: Early repair phases were observed more often in placebo-treated samples (45.5%) than in ASC-CM-treated ones (22.2%). However, the difference was not statistically significant. We demonstrated a statistically significant positive correlation between the early healing phases in tissue samples and C-peptide levels in peripheral blood. The expression of proangiogenic miR-126 was also shown in a number of structures in all phases of ischemic tissue healing. Conclusion: Based on our results, we believe that treatment with ASC-CM has the potential to accelerate the healing process in ischemic tissues in the rabbit model of CLI. The whole healing process was accompanied by miR-126 tissue expression. C-peptide could be used to monitor the course of the tissue healing process.