PT  - JOURNAL ARTICLE
AU  - Tauber, Zdenek
AU  - Cizkova, Katerina
AU  - Janikova, Maria
AU  - Jurcikova, Jana
AU  - Vitkova, Katerina
AU  - Pavliska, Lubomir
AU  - Porubova, Ludmila
AU  - Krauze, Agata
AU  - Fernandez, Carlos
AU  - Jaluvka, Frantisek
AU  - Spackova, Iveta
AU  - Lochman, Ivo
AU  - Prochazka, Martin
AU  - Johnstone, Brian H.
AU  - Prochazka, Vaclav
TI  - Serum C-peptide level correlates with the course of muscle tissue healing in the rabbit model of critical limb ischemia
DP  - 2019 Jun 25
TA  - Biomedical papers
PG  - 132--140
VI  - 163
IP  - 2
AID - 10.5507/bp.2018.048
IS  - 12138118
AB  - Aim: The therapeutic potential of adipose-derived stem cell conditioned medium (ASC-CM) was studied in the rabbit model of critical limb ischemia (CLI). Methods: Rabbits received treatment with ASC-CM or placebo. Gastrocnemius muscle tissue was collected 35 days after ischemia induction. Ischemic changes were evaluated in hematoxylin-eosin stained tissues for early (necrotic lesions/granulation tissue) and late (fibrous scars) phases of tissue repair. The expression of proangiogenic miR-126 was also evaluated using in situ hybridization. The levels of cytokines, insulin, and C-peptide were measured in blood. Results: Early repair phases were observed more often in placebo-treated samples (45.5%) than in ASC-CM-treated ones (22.2%). However, the difference was not statistically significant. We demonstrated a statistically significant positive correlation between the early healing phases in tissue samples and C-peptide levels in peripheral blood. The expression of proangiogenic miR-126 was also shown in a number of structures in all phases of ischemic tissue healing. Conclusion: Based on our results, we believe that treatment with ASC-CM has the potential to accelerate the healing process in ischemic tissues in the rabbit model of CLI. The whole healing process was accompanied by miR-126 tissue expression. C-peptide could be used to monitor the course of the tissue healing process.