RT Journal Article
SR Electronic
A1 Pochop, Pavel
A1 Darsova, Denisa
A1 Uhlik, Jiri
A1 Lestak, Jan
A1 Kukacka, Jiri
A1 Kodetova, Daniela
A1 Klapkova, Eva
A1 Malis, Josef
A1 Vajner, Ludek
T1 Retinal toxicity after repeated intravitreal carboplatin injection into rabbit eyes
JF Biomedical papers
YR 2014
VO 158
IS 4
SP 552
OP 556
DO 10.5507/bp.2012.106
UL https://biomed.papers.upol.cz/artkey/bio-201404-0009.php
AB Background: The aim of this study was to assess retinal toxicity in a rabbit model after carboplatin delivered as repeated transcorneal intravitreal injection, in order to determine the highest possible safe dose for use in human retinoblastoma "seeding" tumor chemotherapy.
Methods and Results: We used six albino rabbits in an in vivo experiment and injected 0.008 mg of carboplatin intravitreally (iv) 4 times at two-week intervals. 0.08 mL saline was injected into the left eye. We recorded electroretinograms (ERGs) before the first carboplatin injection and after the fourth injection. Platinum concentration was measured 1 h after the fifth additional injection. We found reduced dark-adapted b-wave amplitudes and, light-adapted b-wave and a-wave amplitudes. The differences between right and left eyes was significant but we found no histopathologic retinal changes.
Conclusions: 0.008 mg of carboplatin is probably the highest possible safe dose for the treatment of retinoblastoma patients. Questionable is direct extrapolation of retinal toxicity from the rabbit eye model to the human eye.