RT Journal Article
SR Electronic
A1 Hodorova, Ingrid
A1 Solar, Peter
A1 Mihalik, Jozef
A1 Vecanova, Janka
A1 Adamkov, Marian
A1 Rybarova, Silvia
T1 Investigation of tumour supressor protein p53 in renal cell carcinoma patients
JF Biomedical papers
YR 2014
VO 158
IS 1
SP 044
OP 049
DO 10.5507/bp.2012.035
UL https://biomed.papers.upol.cz/artkey/bio-201401-0007.php
AB Background: Investigation of p53 immunoreactivity in formalin-fixed paraffin-embedded tissues of normal renal tissue and renal cell carcinoma with respect to histopathologic subtype and nuclear grade of RCC.
Methods: 42 tissue sections of RCC and 5 samples of normal renal tissue were stained for p53 expression using immunohistochemical assay. The results were analyzed in relation to nuclear grade and histopathologic subtype.
Results: In total, p53 expression was found to be 4 to 5 times higher (30.8%) in other types of RCC than in the clear-cell type of RCC (6.9%). Further, there was no statistically significant difference in p53 overexpression among the histopathologic subtypes (P>0.05, P=0.063). No association was found between the expression of p53 and nuclear grade (P>0.05, P=0.17). Interestingly, our study also showed weak cytoplasmic positivity in renal tubular epithelium.
Conclusion: Our findings suggest that p53 might play an important role in tumour development or progression and it might be used as a new predictor and therapeutic target for RCC.