PT  - JOURNAL ARTICLE
AU  - Pikalova, Zuzana
AU  - Szotkowski, Tomas
AU  - Indrak, Karel
TI  - Immunophenotyping - A tool for the differential diagnostics of lymphadenomegaly
DP  - 2007 Jun 1
TA  - Biomedical papers
PG  - 99--101
VI  - 151
IP  - 1
AID - 10.5507/bp.2007.018
IS  - 12138118
AB  - Backround: Flow cytometry is a method that enables the automated quantification of a set of parameters for a large number of cells or cell-like particles. It is also possible to analyze solid tissues after reduction to a single cell suspension. One of the applications of fl ow cytometry is immunophenotyping.
Aim: The authors try to introduce the basic physical and biological principles of fl ow-cytometry to the broader public and to prove the benefits of method in lymph nodes assessment.
Method: A common method of immunophenotyping with the help of labeled monoclonal antibodies applied to lymph node cells was used.
Results: Flow cytometry revealed the infiltration of the extirpated lymph node with leukemic cells.
Conclusions: The authors demonstrate fl ow cytometry as a relatively less common but efficient way of lymph node assessment in a patient with an anamnesis of acute lymphoblastic leukemia. They also discuss the contribution of immunophenotyping to the process of the differential diagnostics of lymph nodes enlargement.