RT Journal Article
SR Electronic
A1 Anzenbacherová, Eva
A1 Janalík, Jakub
A1 Popa, Igor
A1 Strnad, Miroslav
A1 Anzenbacher, Pavel
T1 INTERACTION OF AROMATIC CYTOKININS WITH HUMAN LIVER MICROSOMAL CYTOCHROMES P450
JF Biomedical papers
YR 2005
VO 149
IS 2
SP 349
OP 351
DO 10.5507/bp.2005.056
UL https://biomed.papers.upol.cz/artkey/bio-200502-0032.php
AB Aromatic cytokinins (ortho-topolin riboside, 6-benzylaminopurine riboside and 6-(2-hydroxy-3-methoxybenzyla mino)purine riboside) were tested for their possible interaction with human liver microsomal cytochromes P450 by absorption difference spectroscopy. All three compounds were shown to bind to the CYP enzymes producing a high to low spin shift of the heme iron yielding a Soret absorption band shift to approximately 425 nm. As this type of spectral change means that the substance is able to bind directly to the heme iron, the results obtained open the possibility of an interaction of these compounds with metabolism of other drugs or, in general, with other substrates of cytochromes P450.