RT Journal Article
SR Electronic
A1 Sauer, Pavel
A1 Gallo, Jiří
A1 Kesselová, Michaela
A1 Kolář, Milan
A1 Koukalová, Dagmar
T1 UNIVERSAL PRIMERS FOR DETECTION OF COMMON BACTERIAL PATHOGENS CAUSING PROSTHETIC JOINT INFECTION
JF Biomedical papers
YR 2005
VO 149
IS 2
SP 285
OP 288
DO 10.5507/bp.2005.043
UL https://biomed.papers.upol.cz/artkey/bio-200502-0019.php
AB The diagnosis of low grade prosthetic joint infection is difficult and time consuming. Nested-PCR for universal bacterial DNA segments detection of "orthopaedic" bacteria was tested in a laboratory setting. This method is based on amplification of the 16S bacterial ribosomal RNA coding sequences. 11 species of the most frequent bacterial pathogens (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus agalactiae, Enterococcus faecium, Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens) involved in prosthetic joint infections were studied. All could be detected rapidly and sensitively by this method.