PT  - JOURNAL ARTICLE
AU  - Sauer, Pavel
AU  - Gallo, Jiří
AU  - Kesselová, Michaela
AU  - Kolář, Milan
AU  - Koukalová, Dagmar
TI  - UNIVERSAL PRIMERS FOR DETECTION OF COMMON BACTERIAL PATHOGENS CAUSING PROSTHETIC JOINT INFECTION
DP  - 2005 Dec 1
TA  - Biomedical papers
PG  - 285--288
VI  - 149
IP  - 2
AID - 10.5507/bp.2005.043
IS  - 12138118
AB  - The diagnosis of low grade prosthetic joint infection is difficult and time consuming. Nested-PCR for universal bacterial DNA segments detection of "orthopaedic" bacteria was tested in a laboratory setting. This method is based on amplification of the 16S bacterial ribosomal RNA coding sequences. 11 species of the most frequent bacterial pathogens (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus agalactiae, Enterococcus faecium, Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens) involved in prosthetic joint infections were studied. All could be detected rapidly and sensitively by this method.