PT Journal
AU Vrba, J
   Modriansky, M
TI N-FORMYL-MET-LEU-PHE-INDUCED OXIDATIVE BURST IN DMSO-DIFFERENTIATED HL-60 CELLS REQUIRES ACTIVE HSP90, BUT NOT INTACT MICROTUBULES
SO Biomedical papers
PY 2004
BP 141
EP 144
VL 148
IS 2
DI 10.5507/bp.2004.025
DE Radicicol/Hsp90/Microtubules/Oxidative burst/HL-60
AB In this study we examined whether microtubules and heat shock protein 90 (Hsp90) are involved in phorbol myristate acetate (PMA) and N-formyl-Met-Leu-Phe (fMLP)-induced oxidative burst in DMSO-differentiated HL-60 cells. Our results showed that microtubule interfering agents, paclitaxel (1-5 uM), colchicine (1-100 uM), nocodazole (1-20 uM), and vincristine (1-50 uM), did not affect either PMA or fMLP-induced oxidative burst. In contrast, radicicol, an inhibitor of Hsp90, inhibited fMLP-induced oxidative burst in time and concentration-dependent manner where IC50 value for 30 min pre-incubation was 16.5 ± 3.5 uM radicicol. We conclude that both PMA and fMLP-induced oxidative burst in DMSO-differentiated HL-60 cells is microtubule-independent while the latter requires Hsp90 activity.
ER