RT Journal Article
SR Electronic
A1 Dvořák, Zdeněk
A1 Pascussi, Jean-Marc
A1 Modrianský, Martin
T1 Approaches to messenger RNA detection - comparison of methods
JF Biomedical papers
YR 2003
VO 147
IS 2
SP 131
OP 135
DO 10.5507/bp.2003.018
UL https://biomed.papers.upol.cz/artkey/bio-200302-0002.php
AB Detection of messenger RNA is an important part of current biomedical research, although utilized for decades. This communication endeavors to compare three most commonly used techniques of mRNA detection, i.e. Northern blot, ribonuclease protection assay (RPA), and real-time polymerase chain reaction (RT-PCR). Principles and general procedures of these methods are described, and advantages and weaknesses of each are discussed in terms of their specificity, sensitivity, difficulty, time and material demands as well as health and environmental risks. We conclude that choice of any method discussed depends on particular purpose, experience of the researcher, and on laboratory equipment and organization.