RT Journal Article SR Electronic A1 Havlickova, Katerina A1 Snopkova, Svatava A1 Pohanka, Miroslav A1 Svacinka, Radek A1 Vydrar, David A1 Husa, Petr A1 Zavrelova, Jirina A1 Zlamal, Filip A1 Fabianova, Lenka A1 Penka, Miroslav A1 Husa, Petr T1 Oxidative stress, microparticles, and E-selectin do not depend on HIV suppression JF Biomedical papers YR 2025 VO 169 IS 2 SP 123 OP 131 DO 10.5507/bp.2024.002 UL https://biomed.papers.upol.cz/artkey/bio-202502-0007.php AB Background. Oxidative stress and inflammation are considered predictors of diseases associated with aging. Markers of oxidative stress, inflammation, and endothelial activation were investigated in people with HIV on antiretroviral treatment to determine whether they had an immunosenescent phenotype that might predispose to the development of premature age-related diseases. Patients and Methods. This study was conducted on 213 subjects with HIV. The control groups consisted of healthy HIV-negative adults. The level of oxidative stress was measured by assessing the production of malondialdehyde levels, which were detected by thiobarbituric acid reactive substance (TBARS) assay. The level of microparticles indicated the presence of inflammation and endothelial activation was measured by E-selectin levels. Significant differences were determined by appropriate statistical tests, depending on the distribution of variables. Relationships between continuous variables were quantified using Spearman's rank correlation coefficient. Results. TBARS, and microparticle and E-selectin levels were significantly higher in untreated and treated subjects with HIV compared with HIV-negative controls (P&lt;0.001). The levels of the investigated markers were not significantly different between untreated and treated patients and no significant correlation of these markers was found with CD4<sup>+</sup> count, CD4<sup>+</sup>/CD8<sup>+</sup> ratio, and the number of HIV-1 RNA copies. Conclusions. Elevated markers of oxidative stress, inflammatory and endothelial activation were independent of the virologic and immunologic status of people with HIV. These results support the hypothesis that residual viremia in cellular reservoirs of various tissues is a key factor related to the premature aging of the immune system and predisposition to the premature development of diseases associated with aging.