RT Journal Article
SR Electronic
A1 Hrabak, Pavel
A1 Zelenkova, Miroslava
A1 Krechler, Tomas
A1 Soupal, Jan
A1 Vocka, Michal
A1 Hanus, Tomas
A1 Petruzelka, Lubos
A1 Svacina, Stepan
A1 Zak, Ales
A1 Zima, Tomas
A1 Kalousova, Marta
T1 Levels of retinol and retinoic acid in pancreatic cancer, type-2 diabetes and chronic pancreatitis
JF Biomedical papers
YR 2024
VO 168
IS 2
SP 132
OP 138
DO 10.5507/bp.2023.049
UL https://biomed.papers.upol.cz/artkey/bio-202402-0005.php
AB Aims. Retinoids participate in multiple key processes in the human body e.g., vision, cell differentiation and embryonic development. There is growing evidence of the relationship between retinol, its active metabolite- all-trans retinoic acid (ATRA) - and several pancreatic disorders. Although low levels of ATRA in pancreatic ductal adenocarcinoma (PDAC) tissue have been reported, data on serum levels of ATRA in PDAC is still limited. The aim of our work was to determine serum concentrations of retinol and ATRA in patients with PDAC, type-2 diabetes mellitus (T2DM), chronic pancreatitis (CHP) and healthy controls. Methods. High performance liquid chromatography with UV detection (HPLC) was used to measure serum levels of retinol and ATRA in 246 patients with different stages of PDAC, T2DM, CHP and healthy controls. Results. We found a significant decrease in the retinol concentration in PDAC (0.44<sup>+/-0.18 </sup>mg/L) compared to T2DM (0.65<sup>+/-0.19 </sup>mg/L, P<0.001), CHP (0.60<sup>+/-0.18 </sup>mg/L, P< 0.001) and healthy controls (0.61<sup>+/-0.15 </sup>mg/L, P<0.001), significant decrease of ATRA levels in PDAC (1.14<sup>+/-0.49 </sup>ug/L) compared to T2DM (1.37<sup>+/-0.56 </sup>ug/L, P<0.001) and healthy controls(1.43<sup>+/-0.55 </sup>ug/L, P<0.001). Differences between early stages (I+II) of PDAC and non-carcinoma groups were not significant. We describe correlations between retinol, prealbumin and transferrin, and correlation of ATRA and IGFBP-2. Conclusion. Significant decrease in retinol and ATRA levels in PDAC compared to T2DM, healthy individuals and/or CHP supports existing evidence of the role of retinoids in PDAC. However, neither ATRA nor retinol are suitable for detection of early PDAC. Correlation of ATRA levels and IGFBP-2 provides new information about a possible IGF and retinol relationship.