RT Journal Article
SR Electronic
A1 Zachova, Katerina
A1 Kosztyu, Petr
A1 Zadrazil, Josef
A1 Matousovic, Karel
A1 Vondrak, Karel
A1 Hubacek, Petr
A1 Kostovcikova, Klara
A1 Tlaskalova Hogenova, Helena
A1 Mestecky, Jiri
A1 Raska, Milan
T1 Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations
JF Biomedical papers
YR 2020
VO 164
IS 3
SP 247
OP 254
DO 10.5507/bp.2019.052
UL https://biomed.papers.upol.cz/artkey/bio-202003-0004.php
AB Aims: Epstein-Barr virus (EBV) targets predominantly B cells and these cells could acquire new phenotype characteristics. Here we analyzed whether EBV-infected and -uninfected B cells from healthy subjects differ in proportion of dominant phenotypes, maturation stage, and homing receptors expression. Methods: EBV-infected and -uninfected cells were identified by flow cytometry using fluorophore-labeled EBV RNA-specific DNA probes combined with fluorophore-labeled antibody to surface lineage markers, integrins, chemokine receptors, and immunoglobulin isotypes, including intracellular ones. Results: Our results show that the trafficking characteristics of EBER<sup>pos</sup> B cells are distinct from EBER<sup>neg</sup> B cells with most dominant differences detected for α4β1 and α4β7 and CCR5 and CCR7. EBV-positive cells are predominantly memory IgM<sup>+</sup> B cells or plasmablasts/plasma cells (PB/PC) positive for IgA or less for IgM. In comparison to uninfected B cells, less EBV-positive B cells express α4β7 and almost no cells express α4β1. EBV-positive B cells contained significantly higher proportion of CCR5<sup>+</sup> and CCR7<sup>+</sup> cells in comparison to EBV-negative cells. In vitro exposure of blood mononuclear cells to pro-inflammatory cytokine IL-6 reduces population of EBV-positive B cell. Conclusion: Although EBV-infected B cells represent only a minor subpopulation, their atypical functions could contribute in predisposed person to development abnormities such as some autoimmune diseases or tumors. Using multi-parameter flow cytometry we characterized differences in migration of EBV-positive and -negative B cells of various maturation stage and isotype of produced antibodies particularly different targeting to mucosal tissues of gastrointestinal and respiratory tracts.