PT  - JOURNAL ARTICLE
AU  - Zachova, Katerina
AU  - Kosztyu, Petr
AU  - Zadrazil, Josef
AU  - Matousovic, Karel
AU  - Vondrak, Karel
AU  - Hubacek, Petr
AU  - Kostovcikova, Klara
AU  - Tlaskalova Hogenova, Helena
AU  - Mestecky, Jiri
AU  - Raska, Milan
TI  - Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations
DP  - 2020 Sep 17
TA  - Biomedical papers
PG  - 247--254
VI  - 164
IP  - 3
AID - 10.5507/bp.2019.052
IS  - 12138118
AB  - Aims: Epstein-Barr virus (EBV) targets predominantly B cells and these cells could acquire new phenotype characteristics. Here we analyzed whether EBV-infected and -uninfected B cells from healthy subjects differ in proportion of dominant phenotypes, maturation stage, and homing receptors expression. Methods: EBV-infected and -uninfected cells were identified by flow cytometry using fluorophore-labeled EBV RNA-specific DNA probes combined with fluorophore-labeled antibody to surface lineage markers, integrins, chemokine receptors, and immunoglobulin isotypes, including intracellular ones. Results: Our results show that the trafficking characteristics of EBER<sup>pos</sup> B cells are distinct from EBER<sup>neg</sup> B cells with most dominant differences detected for α4β1 and α4β7 and CCR5 and CCR7. EBV-positive cells are predominantly memory IgM<sup>+</sup> B cells or plasmablasts/plasma cells (PB/PC) positive for IgA or less for IgM. In comparison to uninfected B cells, less EBV-positive B cells express α4β7 and almost no cells express α4β1. EBV-positive B cells contained significantly higher proportion of CCR5<sup>+</sup> and CCR7<sup>+</sup> cells in comparison to EBV-negative cells. In vitro exposure of blood mononuclear cells to pro-inflammatory cytokine IL-6 reduces population of EBV-positive B cell. Conclusion: Although EBV-infected B cells represent only a minor subpopulation, their atypical functions could contribute in predisposed person to development abnormities such as some autoimmune diseases or tumors. Using multi-parameter flow cytometry we characterized differences in migration of EBV-positive and -negative B cells of various maturation stage and isotype of produced antibodies particularly different targeting to mucosal tissues of gastrointestinal and respiratory tracts.