RT Journal Article
SR Electronic
A1 Dlouhy, Ondrej
A1 Kusnierova, Pavlina
A1 Kurasova, Irena
A1 Cisarikova, Monika
A1 Zeman, David
T1 Chemiluminescent detection of oligoclonal immunoglobulins  after isoelectric focusing and affinity-mediated immunoblotting
JF Biomedical papers
YR 2018
VO 162
IS 2
SP 107
OP 115
DO 10.5507/bp.2018.003
UL https://biomed.papers.upol.cz/artkey/bio-201802-0006.php
AB Background and Aims: Detection of oligoclonal IgG (o-IgG) in the cerebrospinal fluid (CSF) not found in serum is the principal laboratory test to support a diagnosis of multiple sclerosis. The aim of this study was to compare chemiluminescent and chromogenic detection of oligoclonal immunoglobulins in the cerebrospinal fluid and serum after their separation by means of isoelectric focusing followed by immunoblotting.
Methods: A set of experiments was designed to detect oligoclonal immunoglobulins by means of alkaline phosphatase BCIP/NBT substrate and chemiluminescent peroxidase substrate.
Results: Based on visual evaluation of signals, chemiluminescent detection requires about a 4 times lower amount of applied protein than very sensitive BCIP/NBT chromogenic detection. Very good correlation between methods has been shown for oligoclonal IgG. Antigen-specific oligoclonal IgG could be demonstrated by both methods although the pattern was clearer using chemiluminescence. In one patient, oligoclonal IgD bands barely visible by BCIP/NBT were convincingly demonstrated by chemiluminescence.
Conclusion: Chemiluminescent detection is a feasible option for oligoclonal immunoglobulin detection and could be used in cases when the sensitivity needs to be improved. Further studies and method optimisation are warranted.