RT Journal Article
SR Electronic
A1 Handl, Marek
A1 Hermanova, Marketa
A1 Hotarkova, Sylva
A1 Jarkovsky, Jiri
A1 Mudry, Peter
A1 Shatokhina, Tetiana
A1 Vesela, Marcela
A1 Sterba, Jaroslav
A1 Zambo, Iva
T1 Clinicopathological correlation of tumor-associated macrophages in Ewing sarcoma
JF Biomedical papers
YR 2018
VO 162
IS 1
SP 54
OP 60
DO 10.5507/bp.2017.049
UL https://biomed.papers.upol.cz/artkey/bio-201801-0010.php
AB Aims: Tumor-associated macrophages (TAMs) are known markers playing complex roles in tumorigenesis. However, the function of TAMs in a variety of malignancies is not yet fully understood. The aim of this pilot study was to quantify the density of TAMs in Ewing sarcoma and to determine the correlation between TAMs and clinicopathological parameters. Methods: Using immunohistochemistry, the expressions of CD68 and CD163 were examined in 24 tissue samples of Ewing sarcoma of bone. The density of CD68 and CD163-positive TAMs was analyzed quantitatively and semi-quantitatively and statistically correlated with clinical parameters. Results: CD163-positive TAMs outnumbered CD68-positive cells (median of 130 vs 96, respectively). No statistically significant relatio nship was found between density of CD68-positive cells, clinical parameters or prognosis. However, high levels of CD163-positive TAMs were associated with localized disease (P=0.008). In cases with a higher density of CD163-positive cells, a trend toward longer survival was revealed (P=0.063). Conclusion: This is the first study that has quantified CD163 expression in TAMs in Ewing sarcoma and showed its possible prognostic value. CD163 was confirmed to be a more specific marker of macrophages than CD68. CD163 is not an exclusive hallmark of M2 macrophages.